vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-304Research ArticleСтатьиArticlesПРОМОТОРЫ ГЕНОВ РАСТЕНИЙ, УЧАСТВУЮЩИХ В ЗАЩИТЕ ОТ ПАТОГЕНОВPLANT GENE PROMOTERS RESPONSIVE TO PATHOGEN INVASIONСмирноваО. Г.SmirnovaO. G.planta@bionet.nsc.ruКочетовА. В.KochetovA. V.planta@bionet.nsc.ruФедеральное государственное бюджетное учреждение науки Институт цитологии и генетики Сибирского отделения РАН, Новосибирск, Россия Федеральное государственное автономное образовательное учреждение высшего образования Новосибирский национальный исследовательский государственный университет, Новосибирск, РоссияРоссияInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaRussian Federation201419012015184/1765775Copyright © Смирнова О.Г., Кочетов А.В., 20152015Смирнова О.Г., Кочетов А.В.Smirnova O.G., Kochetov A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/304

Генные сети, контролирующие устойчивость растений к различным фитопатогенам, весьма сложны, в них могут принимать участие сотни генов. Инфекция вызывает существенные изменения на молекулярно-генетическом, биохимическом, физиологическом и морфологическом уровнях как локально, в месте инвазии, так и системно. Реконструкция генных сетей, отвечающих за защиту растений от патогенных бактерий, грибов и вирусов, необходима для выявления задействованных молекулярных механизмов, а также для разработки новых способов повышения устойчивости хозяйственно ценных растений. Транскрипционная активность генов, участвующих в защите от фитопатогенов, обычно возрастает в ответ на инфекцию, поэтому характеристика их промоторов является важным источником информации для выявления транскрипционных факторов, контролирующих их работу, и для поиска новых генов, участвующих в ответе на инфекцию. Данные о промоторах необходимы для создания устойчивых к фитопатогенам растений методами генной инженерии. В статье представлены данные о промоторах патоген-чувствительных генов с экспериментально проверенным паттерном экспрессии, аннотированных в базе TGP (TransGene Promoters). База TGP может быть использована в качестве источника информации для интерпретации транскриптомных данных и при планировании генно-инженерных экспериментов, направленных на повышение устойчивости растений к патогенам различного происхождения.

Gene networks controlling plant defense against pathogens are rather complex. They may involve hundreds of genes. Infection induces considerable changes at different levels: molecular-genetic, biochemical, physiological, and morphological. These changes manifest themselves locally (near the invasion site) or systemically. The reconstruction of particular gene networks responsible for defense against pathogenic bacteria, fungi, and viruses is an important step in the elucidation of the underlying molecular mechanisms as well as for the development of new approaches to crop improvement. The transcription levels of genes involved in the defense mechanisms commonly increase in response to pathogen invasion. Thus, investigation of their promoters is important for detection of new transcriptional factors controlling their activity and for search for new genes involved in pathogen response. It seems desirable to employ pathogen-responsive promoters to make plant cultivars resistant to various pathogens by gene engineering techniques. In this paper, we present data on promoters of pathogen-responsive genes with experimentally verified transcription patterns annotated in the TGP (TransGene Promoters) database. TGP may be used as a source of information for both interpretation of transcriptomic data and design of gene engineering constructs to obtain agricultural plants with improved resistance against various pathogens.

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The authors declare that there are no conflicts of interest present.