vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ21.077vavilov-3144Research ArticleБИОРЕСУРСНЫЕ КОЛЛЕКЦИИBIORESOURCE COLLECTIONSГенотипирование образцов картофеля коллекции «ГенАгро» ИЦиГ СО РАН с применением ДНК-маркеров генов устойчивости к фитопатогенамGenotyping of potato samples from the GenAgro ICG SB RAS collection using DNA markers of genes conferring resistance to phytopathogenshttps://orcid.org/0000-0001-5565-9097ТоцкийИ. В.TotskyI. V.

Новосибирск, 

пос. Краснообск, Новосибирская область

Novosibirsk

totsky@bionet.nsc.ruРозановаИ. В.RozanovaI. V.

Новосибирск, 

Санкт-Петербург

Novosibirsk, 

St. Petersburg

СафоноваА. Д.SafonovaA. D.

пос. Краснообск, Новосибирская область

Novosibirsk

БатовА. С.BatovA. S.

пос. Краснообск, Новосибирская область

Novosibirsk

ГурееваЮ. А.GureevaYu. A.

пос. Краснообск, Новосибирская область

Novosibirsk

https://orcid.org/0000-0002-8470-8254ХлесткинаЕ. К.KhlestkinaE. K.

Новосибирск, 

Санкт-Петербург

Novosibirsk, 

St. Petersburg

https://orcid.org/0000-0003-3151-5181КочетовА. В.KochetovA. V.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Сибирский научно-исследовательский институт растениеводства и селекции – филиал Института цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Siberian Research Institute of Plant Production and Breeding – Branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР)РоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian FederationСибирский научно-исследовательский институт растениеводства и селекции – филиал Института цитологии и генетики Сибирского отделения Российской академии наукРоссияSiberian Research Institute of Plant Production and Breeding – Branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian Federation202122102021256677686Copyright © Тоцкий И.В., Розанова И.В., Сафонова А.Д., Батов А.С., Гуреева Ю.А., Хлесткина Е.К., Кочетов А.В., 20212021Тоцкий И.В., Розанова И.В., Сафонова А.Д., Батов А.С., Гуреева Ю.А., Хлесткина Е.К., Кочетов А.В.Totsky I.V., Rozanova I.V., Safonova A.D., Batov A.S., Gureeva Y.A., Khlestkina E.K., Kochetov A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3144

Значительный ущерб урожаю картофеля наносят рак картофеля (болезнь, вызываемая патогенным грибом Synchytrium endobioticum) и золотистая картофельная нематода (Globodera rostochiensis), паразитирующая на корнях растения-хозяина. Оба этих фактора являются объектами внешнего и внутреннего карантина в Российской Федерации, и каждый сорт, регистрируемый в РФ, проходит проверку на устойчивость к наиболее распространенным их расам и патотипам. Основной метод борьбы с подобными заболеваниями – выведение устойчивых сортов. Важным этапом в этом процессе является отбор устойчивых генотипов из популяции и оценка устойчивости гибридов, полученных при скрещиваниях во время селекционного процесса. Проведение постоянной фенотипической оценки связано с рядом трудностей, а именно: не всегда есть возможность работать с патогенами, сама фенотипическая оценка очень затратная и трудоемкая. Однако применение ДНК-маркеров, сцепленных с генами устойчивости, может значительно ускорить и удешевить процесс. Целью исследования было проведение скрининга коллекции картофеля «ГенАгро» (ИЦиГ СО РАН) с использованием ПЦР-маркеров, разработанных для диагностики устойчивости к золотистой картофельной нематоде и раку картофеля. Семьдесят три образца из коллекции «ГенАгро» ИЦиГ СО РАН были генотипированы ДНК-маркерами 57R, CP113, Gro1-4, сцепленными с устойчивостью к нематоде, и маркером NL25 для устойчивости к раку. Результаты генотипирования сопоставлены с уровнем восприимчивости образцов к болезням. Высокий уровень корреляции (коэффициент корреляции Спирмена Spearman R = 0.722008, p = 0.000000, p < 0.05) между устойчивостью и наличием диагностического фрагмента был показан только для маркера 57R. Диагностическая эффективность маркера 57R составила 86.11 %. Данный маркер можно успешно использовать для поиска устойчивых генотипов и проведения маркер-ориентированной селекции. Для остальных маркеров достоверных корреляций не выявлено. Диагностическая эффективность применения маркера CP113 равнялась всего 44.44 %, а коэффициент корреляции Спирмена (Spearman R = –0.109218, p = 0.361104, p < 0.05) показывал отсутствие значимой корреляция между устойчивостью и ДНК-маркером. Диагностическая эффективность маркера NL25 составила 61.11 %. Значимой корреляции между маркером NL25 и устойчивостью не обнаружено (Spearman R = –0.017946, p = 0.881061, p < 0.05). Использование этих маркеров для поиска устойчивых образцов нецелесообразно.

 

Wart (a disease caused by Synchytrium endobioticum) and golden cyst potato nematode (Globodera rostochiensis), which parasitize the roots of the host plant, cause significant damage to potato crop. Both of these disease factors are quarantined in the Russian Federation, and each registered variety is tested for resistance to their most common races and pathotypes. The main method of opposing such diseases is by the development of resistant varieties. An important step in this process is the selection of resistant genotypes from the population and the estimation of the resistance of hybrids obtained by crosses during the breeding process. Conducting a permanent phenotypic evaluation is associated with difficulties, for example, it is not always possible to work with pathogens, and phenotypic evaluation is very costly and time consuming. However, the use of DNA markers linked to resistance genes can significantly speed up and reduce the cost of the breeding process. The aim of the study was to screen the GenAgro potato collection of ICG SB RAS using known diagnostic PCR markers linked to golden potato cyst nematode and wart resistance. Genotyping was carried out on 73 potato samples using three DNA markers 57R, CP113, Gro1-4 associated with nematode resistance and one marker, NL25, associated with wart resistance. The genotyping data were compared with the data on the resistance of the collection samples. Only the 57R marker had a high level of correlation (Spearman R = 0.722008, p = 0.000000, p < 0.05) between resistance and the presence of a diagnostic fragment. The diagnostic efficiency of the 57R marker was 86.11 %. This marker can be successfully used for screening a collection, searching for resistant genotypes and marker-assisted selection. The other markers showed a low correlation between the presence of the DNA marker and resistance. The diagnostic efficiency of the CP113 marker was only 44.44 %. Spearman’s correlation coefficient (Spearman R = –0.109218, p = 0.361104, p < 0.05) did not show significant correlation between resistance and the DNA marker. The diagnostic efficiency of the NL25 marker was 61.11 %. No significant correlation was found between the NL25 marker and resistance (Spearman R = –0.017946, p = 0.881061, p < 0.05). The use of these markers for the search for resistant samples is not advisable.

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The authors declare that there are no conflicts of interest present.