vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ21.085vavilov-3178Research ArticleГЕНЕТИКА РАЗВИТИЯDEVELOPMENTAL GENETICSПолиморфизм последовательностей генов CLE картофеляPolymorphism of CLE gene sequences in potatohttps://orcid.org/0000-0002-9631-6143ГанчеваМ. С.GanchevaM. S.

Санкт-Петербург

St. Petersburg

ganchovai@gmail.comhttps://orcid.org/0000-0003-0968-8684ЛосевМ. Р.LosevM. R.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0002-1791-3063ГуринаА. А.GurinaA. A.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0003-2896-1893ПолюшкевичЛ. О.PoliushkevichL. O.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0001-5282-718XДодуеваИ. Е.DoduevaI. E.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0001-6125-0757ЛутоваЛ. А.LutovaL. A.

Санкт-Петербург

St. Petersburg

Санкт-Петербургский государственный университетРоссияSaint Petersburg State UniversityRussian FederationФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР)РоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian Federation202103122021257746753Copyright © Ганчева М.С., Лосев М.Р., Гурина А.А., Полюшкевич Л.О., Додуева И.Е., Лутова Л.А., 20212021Ганчева М.С., Лосев М.Р., Гурина А.А., Полюшкевич Л.О., Додуева И.Е., Лутова Л.А.Gancheva M.S., Losev M.R., Gurina A.A., Poliushkevich L.O., Dodueva I.E., Lutova L.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3178

CLE (CLV3/ESR) – одна из важнейших групп пептидных фитогормонов. Ее представители регулируют развитие различных органов и тканей растений, а также взаимодействие с некоторыми паразитами и симбионтами и ответ на факторы окружающей среды. В связи с этим идентификация и изучение генов CLE, кодирующих пептиды этой группы, у культурных растений представляют большой практический интерес. О функциях CLE пептидов у картофеля известно немного, поскольку гены CLE картофеля Solanum phureja Juz. et Buk. были охарактеризованы только в 2021 г. Вместе с тем картофель включает в себя много клубненосных видов рода Solanum L., как диких, так и культурных, и разнообразие его форм может зависеть в том числе от различий по последовательностям генов CLE. В этой работе мы впервые произвели поиск и анализ последовательностей генов CLE у трех диких видов картофеля (S. bukasovii Juz. et Rybin., S. verrucosum Schltdl., S. commersonii Dunal.) и четырех культурных (S. chaucha Juz. et Buk., S. curtilobum Juz. et Buk., S. juzepczukii Juz. et Buk., S. ajanhuiri Juz. et Buk.). У проанализированных видов картофеля выявлено 332 гена CLE: от 40 до 43 генов этого семейства для каждого вида картофеля. У всех видов картофеля, взятых в исследование, выявлены гомологи ранее идентифицированных генов CLE S. phureja; в то же время ген CLE42, отсутствующий в геноме S. phureja, найден у всех остальных проанализированных нами видов картофеля. Наибольшие отличия по аминокислотным последовательностям белков CLE оказались характерны для S. commersonii – вида, растущего вне ареалов культурных видов картофеля и, вероятно, не входящего в число их предков. Обнаружены также примеры полиморфизма по аминокислотным последовательностям доменов белков CLE, несущих разную функциональную нагрузку. Дальнейшее изучение белков CLE картофеля позволит выявить их роль в развитии этой важнейшей сельскохозяйственной культуры, в том числе в формировании признаков продуктивности.

CLE (CLV3/ESR) is one of the most important groups of peptide phytohormones: its members regulate the development of various plant organs and tissues, as well as interaction with some parasites and symbionts and response to environmental factors. In this regard, the identification and study of the CLE genes encoding the peptides of this group in cultivated plants are of great practical interest. Relatively little is known about the functions of CLE peptides in potato, since the CLE genes of the potato Solanum phureja Juz. et Buk. were characterized only in 2021. At the same time, potato includes plenty of tuberous species of the genus Solanum L., both wild and cultivated, and the diversity of its forms may depend on differences in the sequences of CLE genes. In this work, we performed a search for and analysis of the CLE gene sequences in three wild potato species (S. bukasovii Juz., S. verrucosum Schltdl., S. commersonii Dunal) and four cultivated species (S. chaucha Juz. et Buk., S. curtilobum Juz. et Buk., S. juzepczukii Juz. et Buk., S. ajanhuiri Juz. et Buk.). In total, we identified 332 CLE genes in the analyzed potato species: from 40 to 43 genes of this family for each potato species. All potato species taken for analysis had homologues of previously identified S. phureja CLE genes; at the same time, the CLE42 gene, which is absent from the S. phureja genome, is present in all other analyzed potato species. Polymorphism of CLE proteins of S. commersonii is significantly higher than that of other analyzed potato species, due to the fact that S. commersonii grows in places outside the growing areas of other potato species and this potato is probably not one of the ancestors of cultivated potato. We also found examples of polymorphism of domains of CLE proteins that carried different functions. Further study of potato CLE proteins will reveal their role in development, including regulation of productivity in this important agricultural crop.

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The authors declare that there are no conflicts of interest present.