vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-318Research ArticleСтатьиArticlesКОМПЬЮТЕРНЫЙ АНАЛИЗ И ФУНКЦИОНАЛЬНАЯ АННОТАЦИЯ САЙТОВ СВЯЗЫВАНИЯ ТРАНСКРИПЦИОННЫХ ФАКТОРОВ AP2/ERF В ГЕНОМЕ ARABIDOPSIS THALIANA L.COMPUTATIONAL ANALYSIS AND FUNCTIONAL ANNOTATION OF AP2/ERF TRANSCRIPTION FACTOR BINDING SITES IN ARABIDOPSIS THALIANA L. GENOMEЧерныхО. А.ChernykhO. A.kviki@bionet.nsc.ruЛевицкийВ. Г.LevitskyV. G.kviki@bionet.nsc.ruОмельянчукН. А.OmelyanchukN. A.kviki@bionet.nsc.ruМироноваВ. В.MironovaV. V.kviki@bionet.nsc.ruФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, РоссияРоссияInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaRussian FederationФедеральное государственное бюджетное учреждение науки Институт цитологии и генетики Сибирского отделения Российской академии наук, Новосибирск, Россия Новосибирский национальный исследовательский государственный университет, Новосибирск, РоссияРоссияInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaRussian Federation201421012015184/2887897Copyright © Черных О.А., Левицкий В.Г., Омельянчук Н.А., Миронова В.В., 20152015Черных О.А., Левицкий В.Г., Омельянчук Н.А., Миронова В.В.Chernykh O.A., Levitsky V.G., Omelyanchuk N.A., Mironova V.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/318

У растений этилен участвует как в регуляции процессов развития, так и в ответе на стрессовые воздействия. Сигнал с рецепторов этилена активирует гены одного из самых больших семейств транскрипционных факторов, APETALA2/ETHYLENE RESPONSE FACTORs (ERFs). Сайты связывания ERF транскрипционных факторов содержат специфический GCCGCC-мотив и называются GCC-боксами. В настоящей работе две компьютерные программы для предсказания сайтов связывания транскрипционных факторов (oPWM и SiteGA) применены для анализа последовательностей экспериментально подтвержденных GCC-боксов. Распознаны GCC-боксы и исследовано их распределение в геноме Arabidopsis thaliana L. Проведены функциональная аннотация распознанных GCC-боксов и анализ их роли в ответе на фитогормон этилен.

The plant hormone ethylene regulates both developmental processes and various stress responses in plants. Ethylene perception in plants is followed by activation of some transcription factors from the large family of APETALA2/ETHYLENE response factors (ERFs). ERF TF binding sites contain a specific GCCGCC motif, called GCC-box. In this study, we applied TF binding site recognition tools oPWM and SiteGA for sequence analysis of experimentally proven GCC-boxes. We carried out GCC box recognition and tested its distribution in the Arabidopsis thaliana L. genome. Functional annotation and microarray data analysis of the genes possessing predicted GCC-boxes elucidated their role in ethylene response.

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The authors declare that there are no conflicts of interest present.