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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJGB-22-04</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-3252</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНОМИКА И ТРАНСКРИПТОМИКА</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT GENETICS AND BREEDING</subject></subj-group></article-categories><title-group><article-title>Особенности побегообразования в популяциях Miscanthus sacchariflorus (Poaceae) под влиянием экологических факторов и паспортизация с помощью ISSR-маркеров</article-title><trans-title-group xml:lang="en"><trans-title>Specific shoot formation in Miscanthus sacchariflorus (Poaceae) under different environmental factors and DNA passportization using ISSR markers</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5729-3594</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дорогина</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Dorogina</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><email xlink:type="simple">olga-dorogina@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0634-8820</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Нуждина</surname><given-names>Н. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Nuzhdina</surname><given-names>N. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7950-054X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Зуева</surname><given-names>Г. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Zueva</surname><given-names>G. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5480-7449</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гисматулина</surname><given-names>Ю. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Gismatulina</surname><given-names>Yu. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0730-3365</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Васильева</surname><given-names>О. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Vasilyeva</surname><given-names>O. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Центральный сибирский ботанический сад Сибирского отделения Российской академии наук;  Новосибирский национальный исследовательский государственный университет<country>Россия</country></aff><aff xml:lang="en">Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Центральный сибирский ботанический сад Сибирского отделения Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru">Институт проблем химико-энергетических технологий Сибирского отделения Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Institute for Problems of Chemical and Energetic Technologies of the Siberian Branch оf the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>01</day><month>03</month><year>2022</year></pub-date><volume>26</volume><issue>1</issue><fpage>22</fpage><lpage>29</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Дорогина О.В., Нуждина Н.С., Зуева Г.А., Гисматулина Ю.А., Васильева О.Ю., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Дорогина О.В., Нуждина Н.С., Зуева Г.А., Гисматулина Ю.А., Васильева О.Ю.</copyright-holder><copyright-holder xml:lang="en">Dorogina O.V., Nuzhdina N.S., Zueva G.A., Gismatulina Y.A., Vasilyeva O.Y.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/3252">https://vavilov.elpub.ru/jour/article/view/3252</self-uri><abstract><p>Уникальным примером травянистых растений, характеризующихся высокими значениями нарастания надземной вегетативной массы и практическим применением в качестве источника альтернативной энергетики, является родовой комплекс Miscanthus Anderss. (Poaceae). Мискантус относится к числу наиболее  эффективных аккумуляторов солнечной энергии, и поскольку фитомелиоративное использование подразумевает выращивание этих ресурсных видов на неудобьях и полутенистых участках, то встает вопрос о влиянии недостаточного освещения на показатели продуктивности мискантуса. В результате длительной интродукции в Центральном сибирском ботаническом саду СО РАН создана перспективная для условий лесостепи Западной Сибири популяция Miscanthus sacchariflorus (Maxim.) Benth. Целью данного исследования было изучение особенностей побегообразования, оценка целлюлозы и лигнина в популяциях M. sacchariflorus при различных условиях освещенности и паспортизация перспективной популяции с помощью ISSR-маркиров. Оценка побегообразования и количества накапливаемой целлюлозы и лигнина у растений проводилась в зависимости от освещенности (один вариант выращивался на солнечном участке, а другой – в полутени). В результате дисперсионного анализа установлено, что число побегов зависит не от экологических условий, а от возраста растения, в то время как на высоту растений существенно воздействуют экологические условия. Несмотря на то что для образцов обоих вариантов M. sacchariflorus была характерна различная скорость создания сплошного проективного покрытия, растения на полутенистых участках образовывали до 89.34 % побегов в сравнении с растениями на освещенных участках, что не оказывало существенного влияния на величину надземной массы и содержание в ней целлюлозы. В результате электрофореза геномной ДНК в популяции M. sacchariflorus при амплификации с пятью ISSR-праймерами выявлены уникальные молекулярные полиморфные фрагменты, которые были применены для идентификации и паспортизации данной популяций. Таким образом, комплексное использование M. sacchariflorus в качестве средоулучшающей и биоэнергетической культуры обусловлено высоким адаптивным потенциалом вида. Обнаружено, что фактор освещенности практически не влияет на количество целлюлозы в стебле, а пониженное содержание технологически нежелательного компонента, лигнина, отмечено при выращивании в условиях полутени.</p></abstract><trans-abstract xml:lang="en"><p>The generic complex Miscanthus Anderss. (Poaceae) is a unique example among herbaceous plants characterized by high values of growth of aboveground vegetative mass and practical use as a valuable source of alternative energy. Miscanthus is one of the most efficient solar energy accumulators, and since phytomeliorative use implies the cultivation of these resource plants in inconvenient and semi-shady areas, the question about the effect of insufficient lighting on the productivity of Miscanthus arises. As a result of a long-lasing introduction effort, the Central Siberian Botanical Garden SB RAS created a population of Miscanthus sacchariflorus (Maxim.) Benth., which has good prospects for growing under the conditions of the forest-steppe area in Western Siberia. The goals of our study were: (1) to determine the peculiarities of shoot formation, (2) to assess the cellulose and lignin accumulation in M. sacchariflorus populations under different lighting conditions and (3) to perform a DNA passportization of the Miscanthus population by ISSR marking. Evaluation of shoot formation and the amount of accumulated cellulose and lignin in plants was carried out under different degrees of illumination: one variant was grown in a sunny area, and the other, in partial shade. As a result of analysis of variance, it was found that the number of shoots does not depend on environmental conditions, but on the age of the plant, while environmental conditions have a significant effect on plant height. Although the samples of both M. sacchariflorus variants were characterized by different rates of creation of a continuous projective cover, plants in semi-shaded areas formed up to 89.34 % of shoots compared to their peers in illuminated areas, which did not affect significantly the size of the aboveground mass and the cellulose content in it. As a result of ISSR-analysis of genomic DNA in the M. sacchariflorus population, unique molecular polymorphic fragments were identified, which can be used for identification and DNA passportization at the inter-population level. Thus, the complex use of M. sacchariflorus as a valuable meliorative and bioenergetic culture is due to the high adaptive potential of this species. It was found that the illumination factor has virtually no effect on the amount of the cellulose content in the shoot, and a reduced content of the technologically undesirable lignin was observed in plants growing in the partial shade conditions.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>род Miscanthus</kwd><kwd>биоэнергетика</kwd><kwd>целлюлоза</kwd><kwd>лигнин</kwd><kwd>побегообразование</kwd><kwd>паспортизация</kwd><kwd>ISSRмаркеры.</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Miscanthus</kwd><kwd>bioenergy</kwd><kwd>cellulose</kwd><kwd>lignin</kwd><kwd>shoot formation</kwd><kwd>DNA passportization</kwd><kwd>ISSR markers</kwd></kwd-group><funding-group xml:lang="en"><funding-statement>The study was carried out according to the complex project of fundamental research program of CSBG SB RAS “Analysis of biodiversity, conservation and restoration of rare and resource plant species using experimental methods” (No. AAAA-A21-121011290025-2). Materials from the bioresource repository “Collections of living plants in open and protected ground” (USU 440534) were used. The study was supported by project FSUS-2021-0012 “Ecosystems of grass pine and small-leaved forests as regulators of the nitrogen and carbon turnovers in the forest-steppe landscape of West Siberia”</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Anisimov A.A., Khokhlov N.F., Tarakanov I.G. Photoperiodic regulation of ontogenesis in different Miscanthus species (Miscanthus spp.). Izvestiya Timiryazevskoy Selskokhozyaystvennoy Akademii = Izvestiya of Timiryazev Agricultural Academy. 2016;6:56-72. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Anisimov A.A., Khokhlov N.F., Tarakanov I.G. Photoperiodic regulation of ontogenesis in different Miscanthus species (Miscanthus spp.). 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