References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

vavilov-33

Research Article

Статьи

Articles

ВЛИЯНИЕ ОТДЕЛЬНЫХ УЧАСТКОВ ХРОМОСОМ TRITICUM TIMOPHEEVII НА ФОРМИРОВАНИЕ УСТОЙЧИВОСТИ К БОЛЕЗНЯМ И КОЛИЧЕСТВЕННЫЕ ПРИЗНАКИ МЯГКОЙ ПШЕНИЦЫ

EFFECT OF CERTAIN CHROMOSOME REGIONS OF TRITICUM TIMOPHEEVII ON THE FORMATION OF PEST RESISTANCE AND QUANTITATIVE TRAITS IN COMMON WHEAT

Тимонова

Е. М.

Timonova

E. M.

eegorova@bionet.nsc.ru

Леонова

И. Н.

Leonova

I. N.

eegorova@bionet.nsc.ru

Белан

И. А.

Belan

I. A.

Россеева

Л. П.

Rosseeva

L. P.

Салина

Е. А.

Salina

E. A.

eegorova@bionet.nsc.ru

Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, РоссияРоссияInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaRussian Federation

Государственное научное учреждение Сибирский научно-исследовательский институт сельского хозяйства Сибирского отделения Российской сельскохозяйственной академии, Омск, РоссияРоссияSiberian Research Institute of Agriculture, Russian Academy of Agricultural Sciences, Novosibirsk, RussiaRussian Federation

2012

10122014

161142159

2014

Тимонова Е.М., Леонова И.Н., Белан И.А., Россеева Л.П., Салина Е.А.

Timonova E.M., Leonova I.N., Belan I.A., Rosseeva L.P., Salina E.A.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/33

Проведена оценка влияния фрагментов интрогрессии Triticum timopheevii Zhuk. (2n = 28, AtAtGG) и их комбинаций на устойчивость к бурой и стеблевой ржавчине, мучнистой росе и ряду количественных признаков у 15 интрогрессивных линий мягкой пшеницы. Анализ интрогрессивных линий молекулярно-генетическими и цитологическими методами продемонстрировал эффективность комплексного использования различных типов маркеров для детальной характеристики гибридных форм и выявления различных транслокаций и замещений. Оценка линий по устойчивости к грибным болезням показала, что линии, содержащие в геноме интрогрессивный фрагмент хромосомы 5G, являются полностью устойчивыми к популяциям бурой ржавчины Западной Сибири и стеблевой ржавчины, типичной для Омской области. Линии 3862-5 и 3862-15, содержащие в геноме фрагмент длинного плеча хромосомы 2G, были устойчивыми к популяции стеблевой ржавчины Западной Сибири. Интрогрессивные линии были изучены по ряду количественных признаков. У всех изученных линий не отмечено негативного влияния чужеродного материала на урожайность и другие количественные признаки, что позволяет их использовать в селекции в качестве доноров устойчивости к грибным заболеваниям. Кроме того, установлено положительное влияние фрагментов хромосомы 2G T. timopheevii на признак озерненности колоса.

The effects of introgression fragments from Triticum timopheevii Zhuk. (2n = 28, AtAtGG) and their combinations on resistance to leaf rust, stem rust, powdery mildew, and some quantitative traits were assessed in 15 common wheat introgression lines. Molecular and cytological analyses of the lines demonstrated an advantage of combined use of various marker types in comprehensive characterization of hybrids and detection of translocations and substitutions. Resistance tests to various fungal diseases showed that the lines containing introgression fragments of chromosome 5G were completely resistant to the West Siberian populations of leaf rust and to the stem rust population of the Omsk region. Lines 3862-5 and 3862-15, containing a fragment of the long arm of chromosome 2G, were resistant to West Siberian stem rust populations. No negative effects of the alien genetic material on yield and other quantitative traits were noted. In addition, positive effect of the 2G chromosome fragments of Triticum timopheevii on ear grain number was established. Thus, the introgression lines can be used in breeding programs as donors of resistance genes to fungal diseases.

интрогрессивные линии мягкой пшеницыT. timopheeviiSSR-анализin situ гибридизацияС-окрашиваниеустойчивость к грибным болезнямколичественные признаки

common wheat introgression linesT. timopheeviiSSR analysisin situ hybridizationC bandingfungus resistancequantitative traits

профессор Marion Röder (Leibniz Institute of Plant Genetics and Crop Plant Research , г. Гатерслебен, Германия), доктор биологических наук Е.Д. Бадаева (Институт молекулярной биологии им. Энгельгарта в Москве), ФЦП РФ

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The authors declare that there are no conflicts of interest present.