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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJGB-22-43</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-3385</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНОМИКА И ТРАНСКРИПТОМИКА</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT GENETICS AND BREEDING</subject></subj-group></article-categories><title-group><article-title>Идентификация гена, кодирующего альбумин семядолей SCA, в геноме гороха (Pisum L.)</article-title><trans-title-group xml:lang="en"><trans-title>Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мглинец</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Mglinets</surname><given-names>A. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Богданова</surname><given-names>В. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Bogdanova</surname><given-names>V. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Костерин</surname><given-names>О. Э.</given-names></name><name name-style="western" xml:lang="en"><surname>Kosterin</surname><given-names>O. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><email xlink:type="simple">kosterin@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>07</day><month>07</month><year>2022</year></pub-date><volume>26</volume><issue>4</issue><fpage>359</fpage><lpage>364</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Мглинец А.В., Богданова В.С., Костерин О.Э., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Мглинец А.В., Богданова В.С., Костерин О.Э.</copyright-holder><copyright-holder xml:lang="en">Mglinets A.V., Bogdanova V.S., Kosterin O.E.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/3385">https://vavilov.elpub.ru/jour/article/view/3385</self-uri><abstract><p>Альбумины SCA и SAA – короткие гидрофильные белки, содержащиеся в высокой концентрации в семядолях и оси семени сухих семян гороха (Pisum sativum L.). Ранее показано, что альбумин SCA имеет два аллельных варианта, различающихся подвижностью в электрофорезе в полиакриламидном геле в кислой среде. С их помощью соответствующий ген SCA картирован в группе сцепления V. Белок SCA был использован как генетический и филогеографический маркер, что до сих пор предполагало проведение электрофореза белков, тогда как последовательность кодирующего гена SCA оставалась неизвестной. На основе данных, доступных в публичных репозиториях, в районе генома гороха, соответствующего позиции гена SCA на генетической карте с учетом синтении геномов гороха и люцерны, осуществлен поиск кандидатов на роль этого гена в зависимости от длины его белкового продукта, положительного заряда в кислых условиях и количества остатков лизина, ранее оцененного электрофоретическими методами. Выявленные гены просеквенированы у ряда образцов гороха. Соответствие полученных электрофоретических данных и нуклеотидной изменчивости позволило идентифицировать последовательность Psat0s797g0160 из референсного генома гороха в качестве гена SCA. Последовательность Psat0s797g0240, возможно, кодирует родственный минорный альбумин SA-a2, тогда как ген-кандидат альбумина SAAостается неидентифицированным (как и электрофоретическая изменчивость двух белков, упомянутых последними). Амплификация ДНК с использованием оригинальных праймеров SCA1_3f и SCA1_3r и геномной ДНК в качестве матрицы, а также расщепление ее эндонуклеазой рестрикции HindII позволяют различать аллели гена SCA, белковые продукты которых имеют разный заряд, без секвенирования. Таким образом, ген, кодирующий высокогидрофильный альбумин SCA, накапливающийся в семядолях гороха, аллели которого полезны для классификации диких родственников культурного гороха, идентифицирован в геноме гороха, и на его основе разработан удобный CAPS-маркер.</p></abstract><trans-abstract xml:lang="en"><p>Albumins SCA and SAA are short, highly hydrophilic proteins accumulated in large quantities in the cotyledons and seed axes, respectively, of a dry pea (Pisum sativum L.) seed. SCA was earlier shown to have two allelic variants differing in mobility in polyacrylamide gel electrophoresis in acid medium. Using them, the corresponding gene SCA was mapped on Linkage Group V. This protein was used as a useful genetic and phylogeographical marker, which still required electrophoretic analysis of the protein while the DNA sequence of the corresponding SCA gene remained unknown. Based on the length, the positive charge under acidic conditions and the number of lysine residues of SCA and SAA albumins, estimated earlier electrophoretically, the data available in public databases were searched for candidates for the SCA gene among coding sequences residing in the region of the pea genome which, taking into account the synteny of the pea and Medicago truncatula genomes, corresponds to the map position of SCA. Then we sequenced them in a number of pea accessions. Concordance of the earlier electrophoretic data and sequence variation indicated the sequence Psat0s797g0160 of the reference pea genome to be the SCA gene. The sequence Psat0s797g0240 could encode a minor related albumin SA-a2, while a candidate gene for albumin SAA is still missing (as well as electrophoretic variation of both latter albumins). DNA amplification using original primers SCA1_3f and SCA1_3r from genomic DNA and restriction by endonuclease HindII made it possible to distinguish the SCA alleles coding for protein products with different charges without sequencing the gene. Thus, the gene encoding the highly hydrophilic albumin SCA accumulated in pea seeds, the alleles of which are useful for classification of pea wild relatives, has now been identified in the pea genome and a convenient CAPS marker has been developed on its basis.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>белки позднего эмбриогенеза</kwd><kwd>альбумины семядолей</kwd><kwd>горох</kwd><kwd>Pisum sativum L.</kwd><kwd>CAPSмаркер</kwd></kwd-group><kwd-group xml:lang="en"><kwd>late embryogenesis proteins</kwd><kwd>seed cotyledon albumin</kwd><kwd>peas</kwd><kwd>Pisum sativum L.</kwd><kwd>CAPS marker</kwd></kwd-group><funding-group xml:lang="en"><funding-statement>The work was supported by the Russian State Scientific Program No. FWNR-2022-0017. Sequencing was carried out in Genomic Core Facility SB RAS, Novosibirsk. Assembly of coding sequences from SRA archives was performed at Siberian Supercomputer Center Core Facility and Computational Facility of Novosibirsk State University. Plants were grown in the greenhouse at the SB RAS Artificial Plant Growing Facility.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Ali S.M., Sharma B., Ambrose M.J. Current status and future strategy in breeding pea to improve resistance to biotic and abiotic stresses. Euphytica. 1994;73:115-126. DOI 10.1007/BF00027188.</mixed-citation><mixed-citation xml:lang="en">Ali S.M., Sharma B., Ambrose M.J. Current status and future strategy in breeding pea to improve resistance to biotic and abiotic stresses. Euphytica. 1994;73:115-126. 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