vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-22-52vavilov-3433Research ArticleСЕЛЕКЦИЯ РАСТЕНИЙ НА ПРОДУКТИВНОСТЬПовышение эффективности клонального микроразмножения картофеля при инокуляции ризосферными бактериями Azospirillum baldaniorum Sp245 и Ochrobactrum cytisi IPA7.2Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2https://orcid.org/0000-0002-6040-9401КаргаполоваК. Ю.KargapolovaK. Yu.

Саратов

Saratov

https://orcid.org/0000-0001-8327-6763ТкаченкоО. В.TkachenkoO. V.

Саратов

Saratov

oktkachenko@yandex.ruhttps://orcid.org/0000-0001-8031-9641БурыгинГ. Л.BuryginG. I.

Саратов

Saratov

https://orcid.org/0000-0002-3973-6766ЕвсееваН. В.EvseevaN. V.

Саратов

Saratov

https://orcid.org/0000-0003-4321-735XШироковА. А.ShirokovA. A.

Саратов

Saratov

https://orcid.org/0000-0001-5654-8292МатораЛ. Ю.MatoraL. Yu.

Саратов

Saratov

https://orcid.org/0000-0002-1084-312XЩёголевС. Ю.ShchyogolevS. Yu.

Саратов

Saratov

Саратовский государственный аграрный университет им. Н.И. ВавиловаРоссияSaratov State Vavilov Agrarian UniversityRussian FederationСаратовский государственный аграрный университет им. Н.И. Вавилова; Институт биохимии и физиологии растений и микроорганизмов – обособленное структурное подразделение Федерального исследовательского центра «Саратовский научный центр Российской академии наук»РоссияSaratov State Vavilov Agrarian University; Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Saratov Federal Scientific Centre of the Russian Academy of SciencesRussian FederationИнститут биохимии и физиологии растений и микроорганизмов – обособленное структурное подразделение Федерального исследовательского центра «Саратовский научный центр Российской академии наук»РоссияInstitute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Saratov Federal Scientific Centre of the Russian Academy of SciencesRussian Federation202201092022265422430Copyright © Каргаполова К.Ю., Ткаченко О.В., Бурыгин Г.Л., Евсеева Н.В., Широков А.А., Матора Л.Ю., Щёголев С.Ю., 20222022Каргаполова К.Ю., Ткаченко О.В., Бурыгин Г.Л., Евсеева Н.В., Широков А.А., Матора Л.Ю., Щёголев С.Ю.Kargapolova K.Y., Tkachenko O.V., Burygin G.I., Evseeva N.V., Shirokov A.A., Matora L.Y., Shchyogolev S.Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3433

Устойчивое развитие сельского хозяйства зависит от обеспечения рынка качественными семенами. Инокуляция растений рост-стимулирующими ризобактериями в культуре in vitro может быть использована для повышения эффективности роста и продуктивности микрорастений при получении оздоровленного посадочного материала картофеля. Изучено влияние инокуляции in vitro штаммами Azospirillum baldaniorum Sp245 и Ochrobactrum cytisi IPA7.2 по отдельности и в консорциуме на микрорастения сортов Невский и Кондор. Оценены морфологические параметры роста растений в культуре in vitro, в условиях адаптации ex vitro, а также показатели роста и продуктивности растений в грунтовой теплице. На протяжении всего опыта была установлена зависимость эффективности бактеризации от генотипа картофеля, этапа культивирования и состава инокулята. Методом иммунофлуоресцентного анализа показано, что оба штамма бактерий успешно вступают во взаимодействие с клетками растений без антагонистического взаимного влияния. В культуре in vitro A. baldаniorum Sр245 и консорциум штаммов стимулировали образование корней на микрорастениях обоих сортов и рост побегов сорта Невский. На этапе культивирования ex vitro на все ростовые показатели микрорастений сорта Невский положительно влияла инокуляция O. cytisi IPA7.2 и консорциум штаммов. При выращивании в теплице в большинстве вариантов инокуляции стимулировался рост побегов обоих сортов. Приживаемость растений сорта Невский в теплице повысилась под действием одновременной коинокуляции в 1.7 раза. Инокуляция микрорастений консорциумом штаммов A. baldаniorum Sр245 и O. cytisi IPA7.2 увеличивала количество мини-клубней у сорта Невский в 1.5 раза, а у сорта Кондор – в 3.5 раза. Инокуляция изученными штаммами может быть использована для стимулирования роста микрорастений и повышения урожайности мини-клубней в системе семеноводства картофеля при получении оздоровленного посадочного материала.

Sustainable development of agriculture depends on the provision of quality seeds to the market. Inoculation with plant-growth-promoting rhizobacteria in in vitro culture can be used to improve the growth efficacy and performance of microplants. We examined the effect of in vitro inoculation of microplants of the cultivars Nevsky and Kondor with the strains Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2 separately and in combination. We examined the morphological variables of plant growth in in vitro culture and under ex vitro adaptation conditions; we also investigated the growth and performance of the plants in the greenhouse. The dependence of the inoculation eff icacy on potato genotype, growth stage, and inoculum composition was ascertained throughout the experiment. In vitro, A. baldaniorum Sp245 alone and in combination with O. cytisi IPA7.2 promoted the formation of roots on the microplants of both cultivars and the growth of Nevsky shoots. During plant growth ex vitro, all growth variables of the Nevsky microplants were promoted by O. cytisi IPA7.2 alone and in combination with A. baldaniorum Sp245. In both cultivars grown in the greenhouse, shoot growth was promoted in most inoculation treatments. The survival ability of the Nevsky microplants in the greenhouse increased 1.7-fold under the effect of simultaneous inoculation. Inoculation of microplants with a combination of A. baldaniorum Sp245 and O. cytisi IPA7.2 increased the number of Nevsky minitubers 1.5-fold and the number of Kondor minitubers 3.5-fold. Inoculation with the tested strains can be used to promote the growth of microplants and increase the yield of minitubers in potato seed breeding for the production of healthy planting material.

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The authors declare that there are no conflicts of interest present.