vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-22-60vavilov-3442Research ArticleМАТЕРИАЛЫ КОНФЕРЕНЦИИ «МЕХАНИЗМЫ АДАПТАЦИИ МИКРООРГАНИЗМОВ», ИРКУТСКВлияние антимикробных препаратов на биопленкообразование Pseudomonas aeruginosaEffects of аntimicrobials on Pseudomonas aeruginosa biofilm formationhttps://orcid.org/0000-0002-7656-342XНемченкоУ. М.NemchenkoU. M.

Иркутск

Irkutsk

umnemch@mail.ruhttps://orcid.org/0000-0001-7717-906XСитниковаК. О.SitnikovaK. O.

Иркутск

Irkutsk

https://orcid.org/0000-0001-9720-068XБельковаН. Л.BelkovaN. L.

Иркутск

Irkutsk

nlbelkova@gmail.comhttps://orcid.org/0000-0001-6588-2591ГригороваЕ. В.GrigorovaE. V.

Иркутск

Irkutsk

https://orcid.org/0000-0001-7026-2522ВоропаеваН. М.VoropaevaN. M.

Иркутск

Irkutsk

СухореваМ. В.SukhоrevaM. V.

Иркутск

Irkutsk

СухареваЕ. С.SukharevaE. S.

Иркутск

Irkutsk

https://orcid.org/0000-0002-9217-6876СавиловЕ. Д.SavilovE. D.

Иркутск

Irkutsk

Научный центр проблем здоровья семьи и репродукции человекаРоссияScientific Сentre for Family Health and Human Reproduction ProblemsRussian FederationГородская Ивано-Матренинская детская клиническая больницаРоссияCity Ivano-Matreninskaya Children’s Clinical HospitalRussian FederationНаучный центр проблем здоровья семьи и репродукции человека; Иркутская государственная медицинская академия последипломного образования – филиал Российской медицинской академии непрерывного профессионального образования Министерства здравоохранения Российской ФедерацииРоссияScientific Сentre for Family Health and Human Reproduction Problems; Irkutsk State Medical Academy of Postgraduate Education – Branch Campus of the Russian Medical Academy of Continuing Professional Education of the Ministry of Healthcare of the Russian FederationRussian Federation202203092022265495501Copyright © Немченко У.М., Ситникова К.О., Белькова Н.Л., Григорова Е.В., Воропаева Н.М., Сухорева М.В., Сухарева Е.С., Савилов Е.Д., 20222022Немченко У.М., Ситникова К.О., Белькова Н.Л., Григорова Е.В., Воропаева Н.М., Сухорева М.В., Сухарева Е.С., Савилов Е.Д.Nemchenko U.M., Sitnikova K.O., Belkova N.L., Grigorova E.V., Voropaeva N.M., Sukhоreva M.V., Sukhareva E.S., Savilov E.D.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3442

Синегнойная палочка (Pseudomonas aeruginosa) относится к наиболее проблемным патогенам в лечебных учреждениях, что может быть связано со способностью этого микроорганизма существовать в биопленке, которая повышает его устойчивость к антимикробным препаратам, а также распространенность и выживаемость во внешней среде. Цель настоящей работы – оценка чувствительности штаммов P. aeruginosa, находящихся в планктонной форме и форме биопленки, к воздействию антимикробных препаратов. Исследовано 20 штаммов P. aeruginosa из рабочей коллекции лаборатории микробиома и микроэкологии Научного центра проблем здоровья семьи и репродукции человека, собранной в течение 2018–2021 гг. Идентификация штаммов проведена с использованием тест-систем для дифференциации грамотрицательных неферментирующих бактерий и подтверждена масс-спектрометрическим анализом и секвенированием гена 16S рРНК. Активность антимикробных препаратов оценивали по степени ингибирования роста клеток бактерий, находящихся в планктонной форме и форме биопленки. Установлено, что все клинические штаммы P. aeruginosa были биопленкообразующими, 47.6 % относились к слабообразующим, 52.4 % – к умереннообразующим. Планктонные клетки и формирующаяся биопленка тестируемых штаммов были устойчивы к карбапенемам. Формирование биопленки в более чем 90 % случаев подавляло препараты групп цефалоспоринов и аминогликозидов. Чувствительность к воздействию антимикробных препаратов у штаммов P. aeruginosa, находящихся в сформированной биопленке, была значимо ниже (p < 0.05). Карбапенемы и цефалоспорины не воздействовали на зрелые биопленки тестируемых штаммов P. aeruginosa более чем в 60 % случаев. Только не-бета-лактамные антибиотики (ципрофлоксацин и амикацин) подавляли рост планктонных клеток и разрушали зрелую биопленку. Выявленные различия в действии испытанных препаратов на биопленку штаммов P. aeruginоsa коррелируют с устойчивостью к целому ряду антибиотиков. Для предупреждения формирования биопленок у больничных штаммов P. аeruginosa может быть рекомендовано применение цефтазидима, для воздействия на зрелые биопленки P. аeruginosa – антимикробные препараты ципрофлоксацин и амикацин.

Pseudomonas aeruginosa is one of the most problematic pathogens in medical institutions, which may be due to the ability of this microorganism to exist in a biofilm, which increases its resistance to antimicrobials, as well as its prevalence and survival ability in the external environment. This work aimed to evaluate the antimicrobial susceptibility of P. aeruginosa strains in planktonic and biofilm forms. We studied 20 strains of P. aeruginosa collected during 2018–2021 by specialists from the Laboratory of Microbiome and Microecology of the Scientific Centre for Family Health and Human Reproduction Problems. The identification of strains was carried out using test systems for differentiating gram-negative non-fermenting bacteria (NEFERMtest 24 Erba Lachema s.r.o., Czech Republic), and confirmed by mass spectrometric analysis and 16S rRNA gene sequencing. Antimicrobial activity was assessed by the degree of inhibition of cell growth in planktonic and biofilm forms (on a flat-bottomed 96-well plastic immunological plate). All clinical isolates of P. aeruginosa were biofilm formers, 47.6 % of the isolates were weak biofilm formers, and 52.4 % of the isolates were moderate biofilm formers. Planktonic cells and the forming biofilm of the tested P. aeruginosa strains were carbapenems-resistant. Biofilm formation was suppressed in more than 90 % of cases by the agents of the cephalosporin and aminoglycoside groups. Antimicrobial susceptibility of P. aeruginosa strains in the formed biofilm was significantly lower (p < 0.05). Carbapenems and cephalosporins did not affect the mature biofilms of the tested P. aeruginosa strains in more than 60 % of cases. Only non-beta-lactam antibiotics (ciprofloxacin and amikacin) suppressed the growth of planktonic cells and destroyed the mature biofilm. The revealed differences in the effect of the tested antimicrobials on the P. aeruginosa strains biofilms correlate with resistance to a number of antibiotics. To prevent biofilm formation in the hospital strains of P. aeruginosa, the use of ceftazidime may be recommended, and antimicrobials such as ciprofloxacin and amikacin may be used to affect mature biofilms of P. aeruginosa.

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The authors declare that there are no conflicts of interest present.