vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-22-69vavilov-3478Research ArticleГЕНЕТИКА МИКРООРГАНИЗМОВMICROBIAL GENETICSВлияние колхицина на физиолого-биохимические свойства Rhodococcus qingshengiiEffect of colchicine on physiological and biochemical properties of Rhodococcus qingshengiihttps://orcid.org/0000-0001-7767-4204МарковаЮ. А.MarkovaYu. A.

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Irkutsk

juliam06@mail.ruhttps://orcid.org/0000-0001-5922-3397БеловежецЛ. А.BelovezhetsL. A.

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Irkutsk

https://orcid.org/0000-0001-5922-3397НурминскийВ. Н.NurminskyV. N.

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Irkutsk

https://orcid.org/0000-0002-6495-1783КапустинаИ. С.KapustinaI. S.

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Irkutsk

https://orcid.org/0000-0002-0436-8166ОзолинаН. В.OzolinaN. V.

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https://orcid.org/0000-0001-7552-0818ГуринаВ. В.GurinaV. V.

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https://orcid.org/0000-0002-3445-0558РакевичА. Л.RakevichA. L.

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https://orcid.org/0000-0003-4948-4301СидоровА. В.SidorovA. V.

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Irkutsk

Сибирский институт физиологии и биохимии растений Сибирского отделения Российской академии наукРоссияSiberian Institute of Plant Physiology and Biochemistry of Siberian Branch of Russian Academy of SciencesRussian FederationИркутский институт химии им. А.Е. Фаворского Сибирского отделения Российской академии наукРоссияA.E. Favorsky Irkutsk Institute of Chemistry of the Siberian Branch of the Russian Academy of SciencesRussian FederationСибирский институт физиологии и биохимии растений Сибирского отделения Российской академии наук,РоссияSiberian Institute of Plant Physiology and Biochemistry of Siberian Branch of Russian Academy of SciencesRussian FederationИркутский филиал Института лазерной физики Сибирского отделения Российской академии наукРоссияIrkutsk Branch of the Institute of Laser Physics, The Siberian Branch of the Russian Academy of SciencesRussian Federation202209102022266568574Copyright © Маркова Ю.А., Беловежец Л.А., Нурминский В.Н., Капустина И.С., Озолина Н.В., Гурина В.В., Ракевич А.Л., Сидоров А.В., 20222022Маркова Ю.А., Беловежец Л.А., Нурминский В.Н., Капустина И.С., Озолина Н.В., Гурина В.В., Ракевич А.Л., Сидоров А.В.Markova Y.A., Belovezhets L.A., Nurminsky V.N., Kapustina I.S., Ozolina N.V., Gurina V.V., Rakevich A.L., Sidorov A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3478

Род Rhodococcus объединяет полиморфные неспорообразующие грамположительные бактерии, относящиеся к классу Actinobacteria. Rhodococcus вместе с Mycobacterium и Corynebacterium входят в группу Mycolata. Благодаря относительно высокой скорости роста и способности к образованию биопленок, Rhodococcus являются удобной моделью для изучения действия биологически активных соединений на патогенные Mycolata. Ранее было показано, что колхицин угнетал образование биопленок у P. carotovorum ВКМ В-1247 и R. quingshengii ВКМ Ac-2784D. Целью настоящей работы было изучение действия колхицина на жирнокислотный состав и микровязкость мембран Rhodococcus qingshengii ВКМ Ac-2784D для понимания механизма действия этого алкалоида на бактериальную клетку. В качестве положительного контроля использовали нистатин, снижающий микровязкость мембран. Установлено, что колхицин в концентрациях 0.01 и 0.03 г/л и нистатин (0.03 г/л) не оказали существенного влияния на выживаемость R. qingshengii ВКМ Ac-2784D, культивируемого в забуференном физиологическом растворе глюкозы (ЗФРГ). Однако колхицин (0.03 г/л) значительно угнетал образование биопленки. Клетки Rhodococcus, культивируемые в течение суток в ЗФРГ с колхицином, приобретали округлую форму. Колхицин в концентрации 0.01 г/л вызывал увеличение жирных кислот C16:1(n-7), С17:0, C20:1(n-9) и C21:0. Микровязкость мембраны отдельных клеток распределялась от максимально низких до максимально высоких значений показателя обобщенной поляризации флуоресценции лаурдана (GP), что свидетельствует о разнообразии адаптационных ответов на этот алкалоид. При более высокой концентрации колхицина (0.03 г/л) в мембранах клеток R. qingshengii ВКМ Ac-2784D увеличивалось содержание насыщенных жирных кислот и падало – разветвленных. Это способствовало увеличению микровязкости мембраны, что подтверждается данными по GP. Таким образом, колхицин индуцирует перестройку клеточной мембраны Rhodococcus, вероятно, в сторону увеличения ее микровязкости, что может быть одной из причин негативного действия колхицина на образование биопленок R. qingshengii ВКМ Ac-2784D.

The genus Rhodococcus includes polymorphic non-spore-forming gram-positive bacteria belonging to the class Actinobacteria. Together with Mycobacterium and Corynebacterium, Rhodococcus belongs to the Mycolata group. Due to their relatively high growth rate and ability to form biof ilms, Rhodococcus are a convenient model for studying the effect of biologically active compounds on pathogenic Mycolata. Colchicine was previously found to reduce biof ilm formation by P. carotovorum VKM B-1247 and R. qingshengii VKM Ac-2784D. To understand the mechanism of action of this alkaloid on the bacterial cell, we have studied the change in the fatty acid composition and microviscosity of the R. qingshengii VKM Ac-2784D membrane. Nystatin, which is known to reduce membrane microviscosity, is used as a positive control. It has been found that colchicine at concentrations of 0.01 and 0.03 g/l and nystatin (0.03 g/l) have no signif icant effect on the survival of R. qingshengii VKM Ac-2784D cultivated in a buffered saline solution with 0.5 % glucose (GBSS). However, colchicine (0.03 g/l) signif icantly inhibits biof ilm formation. Rhodococcus cells cultivated for 24 hours in GBSS with colchicine acquire a rounded shape. Colchicine at 0.01 g/l concentration increases C16:1(n-7), C17:0, C20:1(n-9) and C21:0 fatty acids. The microviscosity of the membrane of individual cells was distributed from the lowest to the highest values of the generalized laurdan f luorescence polarization index (GP), which indicates a variety of adaptive responses to this alkaloid. At a higher concentration of colchicine (0.03 g/l) in the membranes of R. qingshengii VKM Ac-2784D cells, the content of saturated fatty acids increases and the content of branched fatty acids decreases. This contributes to an increase in membrane microviscosity, which is conf irmed by the data on the GP fluorescence of laurdan. All of the above indicates that colchicine induces a rearrangement of the Rhodococcus cell membrane, probably in the direction of increasing its microviscosity. This may be one of the reasons for the negative effect of colchicine on the formation of R. qingshengii VKM Ac-2784D biof ilms.

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The authors declare that there are no conflicts of interest present.