vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-22-78vavilov-3529Research ArticleГЕНЕТИЧЕСКИЕ РЕСУРСЫ РАСТЕНИЙPLANT GENETICS RESOUCESПолифенолы Perilla frutescens семейства Lamiaceae, идентифицированные с помощью тандемной масс-спектрометрииPolyphenols of Perilla frutescens of the family Lamiaceae identified by tandem mass spectrometryhttps://orcid.org/0000-0002-9732-1649РазгоноваМ. П.RazgonovaM. P.

Санкт-ПетербургВладивосток

St. PetersburgVladivostok

https://orcid.org/0000-0002-4920-3904КоньковаН. Г.Kon’kovaN. G.

Санкт-Петербург

St. Petersburg

ninakonkova.1@mail.ruhttps://orcid.org/0000-0002-9520-8271ЗахаренкоА. М.ZakharenkoA. M.

р.п. Краснообск, Новосибирская областьТомск

Krasnoobsk, Novosibirsk RegionTomsk

https://orcid.org/0000-0002-4873-2281ГолохвастК. С.GolokhvastK. S.

Санкт-ПетербургВладивостокр.п. Краснообск, Новосибирская областьТомск

St. PetersburgVladivostokKrasnoobsk, Novosibirsk RegionTomsk

Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР); Дальневосточный федеральный университетРоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR); Far Eastern Federal University;Russian FederationФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР)РоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian FederationСибирский федеральный научный центр агробиотехнологий Российской академии наук; Томский государственный университетРоссияSiberian Federal Scientific Centre of Agro-BioTechnology of the Russian Academy of Sciences; Tomsk State UniversityRussian FederationФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР); Дальневосточный федеральный университет; Сибирский федеральный научный центр агробиотехнологий Российской академии наук; Томский государственный университетРоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR); Far Eastern Federal University; Siberian Federal Scientific Centre of Agro-BioTechnology of the Russian Academy of Sciences; Tomsk State UniversityRussian Federation202228112022267637644Copyright © Разгонова М.П., Конькова Н.Г., Захаренко А.М., Голохваст К.С., 20222022Разгонова М.П., Конькова Н.Г., Захаренко А.М., Голохваст К.С.Razgonova M.P., Kon’kova N.G., Zakharenko A.M., Golokhvast K.S.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3529

Perilla frutescens получила применение в основном как масличная культура. Семена периллы содержат 40–53 % масла, 28 % белка. Вегетационный период составляет 100–150 дней. В России периллу выращивают на Дальнем Востоке, где урожайность достигает 0.8–1.2 т/га. Это растение короткого дня, поэтому большинство форм не цветет в условиях средней полосы России. Перилла различного географического происхождения имеет свои особенные, резко отличные признаки, характеризующие две географические группы: японскую и корейско-китайскую. Эти группы различаются длиной вегетационного периода, а также по высоте растений, окраске стебля, поверхности и величине листьев, форме куста, форме и размеру соцветий, величине чашечек и цвету семян. Perilla frutescens содержит большое количество полифенольных соединений, которые являются биологически активными компонентами. Цель данной работы состояла в метаболомном исследовании экстрактов из листьев P. frutescens, полученных из коллекции Всероссийского института генетических ресурсов растений им. Н.И. Вавилова и выращенных на полях его Дальневосточной опытной станции (Приморский край, Россия). Для идентификации целевых аналитов в экстрактах использовали метод высокоэффективной жидкостной хроматографии в сочетании с ионной ловушкой. Предварительные результаты показали наличие 23 биологически активных соединений, соответствующих виду P. frutescens. В дополнение к упомянутым метаболитам, в экстрактах P. frutescens был впервые обнаружен ряд соединений. Это кумарин умбеллиферон; тритерпен сквален; стеаридоновая кислота; высокомолекулярные карбоновые кислоты: тетракозановая кислота и сальвиановая кислота C; лигнан сирингарезинол; циклобутановый лигнан сагериновая кислота и др. Широкий спектр биологически активных соединений открывает богатые возможности для создания новых лекарственных средств и биологически активных добавок на основе экстрактов периллы семейства Lamiaceae, подсемейства Lamioideae, трибы Satureji и подтрибы Perillinae.

Perilla frutescens is mainly cultivated as an oilseed crop. Perilla seeds contain 40–53 % of oil, 28 % of protein. The growing season is 100–150 days. In Russia, perilla is grown in the Far East, where the yield is 0.8–1.2 t/ha. Perilla of different geographical origin has its own special, sharply different features that characterize two geographical groups: Japanese and Korean-Chinese. These groups differ from each other in the length of the growing season, the height of plants, the color of the stem, the surface and the size of the leaves, the shape of the bush, the shape and size of the inflorescences, the size of the cups, the size and color of the seeds. P. frutescens contains a large number of polyphenolic compounds that are biologically active components. The purpose of this research was a metabolomic study of extracts from leaves of P. frutescens obtained from the collection of Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources, grown on the fields of the Far East Experiment Station – Branch of Federal Research Center (Primorsky Krai, Russia). To identify target analytes in extracts, HPLC was used in combination with an ion trap. Preliminary results showed the presence of 23 biologically active compounds corresponding to P. frutescens. In addition to the reported metabolites, a number of metabolites were newly annotated in P. frutescens. There were hydroxycoumarin Umbelliferone; triterpene Squalene; omega-3 fatty acid Stearidonic [Moroctic] acid; higher-molecular-weight carboxylic acid: Tetracosenoic acid and Salvianic acid C; lignan Syringaresinol and cyclobutane lignan Sagerinic acid, etc. A wide range of biologically active compounds opens up rich opportunities for the creation of new drugs and dietary supplements based on extracts of perilla of the family Lamiaceae, subfamily Lamioideae, tribe Satureji and subtribe Perillinae.

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The authors declare that there are no conflicts of interest present.