vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-22-80vavilov-3534Research ArticleМОЛЕКУЛЯРНЫЕ МАРКЕРЫ В ГЕНЕТИКЕ И СЕЛЕКЦИИMOLECULAR MARKERS IN GENETICS AND BREEDINGВариабельность генома отечественных сортов томата: данные AFLP-анализаGenome variability of domestic tomato varieties: data from AFLP analysishttps://orcid.org/0000-0002-3124-525XКулаковаА. В.KulakovaA. V.

Москва

Moscow

kulakova_97@mail.ruhttps://orcid.org/0000-0002-0570-9751ДьяченкоЕ. А.DyachenkoE. A.

Москва

Moscow

https://orcid.org/0000-0003-4692-3727ЩенниковаА. В.ShchennikovaA. V.

Москва

Moscow

https://orcid.org/0000-0001-9744-2443ПышнаяО. Н.PyshnayaO. N.

пос. ВНИИССОК, Московская область

VNIISSOK, Moscow region

https://orcid.org/0000-0002-2216-0094ДжосЕ. А.DzhosE. A.

Москвапос. ВНИИССОК, Московская область

MoscowVNIISSOK, Moscow region

Институт биоинженерии, Федеральный исследовательский центр «Фундаментальные основы биотехнологии» Российской академии наукРоссияInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of SciencesRussian FederationФедеральный научный центр овощеводстваРоссияFederal Scientific Vegetable Center,Russian FederationИнститут биоинженерии, Федеральный исследовательский центр «Фундаментальные основы биотехнологии» Российской академии наук; Федеральный научный центр овощеводстваРоссияInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences; Federal Scientific Vegetable Center,Russian Federation202229112022267652661Copyright © Кулакова А.В., Дьяченко Е.А., Щенникова А.В., Пышная О.Н., Джос Е.А., 20222022Кулакова А.В., Дьяченко Е.А., Щенникова А.В., Пышная О.Н., Джос Е.А.Kulakova A.V., Dyachenko E.A., Shchennikova A.V., Pyshnaya O.N., Dzhos E.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3534

Томат Solanum lycopersicum L. является одной из основных овощных культур, образцы и сорта которой характеризуются низким уровнем геномного полиморфизма. В интрогрессивной селекции томата используют родственные дикорастущие виды Solanum для улучшения сортов по признакам устойчивости к стрессовым факторам и качества плодов. Целью работы была оценка вариабельности генома 59 сортов и перспективных селекционных линий S. lycopersicum и 11 дикорастущих видов томата с помощью метода AFLP. По данным AFLPанализа было выбрано четыре комбинации праймеров E32/M59, E32/M57, E38/M57 и Е41/М59, которые отличались наиболее высокими показателями PIC (polymorphism information content). В процессе маркирования коллекции из 59 сортов/линий S. lycopersicum и 11 дикорастущих образцов томата отобранными праймерами выявлен 391 фрагмент размером от 80 до 450 п.н., из которых 114 фрагментов оказались полиморфными и 25 – уникальными. Анализ спектров амплификации выделил дикорастущие образцы томата в отдельные клады. Сестринские клады включали сорта селекции Федерального научного центра овощеводства, устойчивые к засухе и/или холоду и, частично, к фитофторозу, альтернариозу, септориозу, вирусу табачной мозаики и вершинной гнили плода, а также не охарактеризованные по данным признакам образцы томата, что позволяет предположить наличие у них устойчивости к стрессовым факторам. У сортовых образцов отдаленных клад присутствует кластеризация по признакам устойчивости к вертициллезу, кладоспориозу, фузариозу, вирусу табачной мозаики, серой гнили и вершинной гнили плода. Показано объединение образцов согласно их происхождению от организации-оригинатора. Продемонстрирована перспективность праймерных комбинаций E32/M59, E32/M57, E38/M57 и Е41/М59 для генотипирования сортов томата с целью отбора доноров устойчивости к различным стрессовым факторам. Выявленные в настоящей работе кладоспецифичные фрагменты могут стать основой для разработки AFLP-маркеров для признаков устойчивости к стрессовым факторам.

Tomato Solanum lycopersicum L. is one of the main vegetable crops, accessions and cultivars of which are characterized by a low level of genomic polymorphism. Introgressive tomato breeding uses related wild Solanum species to improve cultivars for stress tolerance and fruit quality traits. The aim of this work was to evaluate the genome variability of 59 cultivars and perspective breeding lines of S. lycopersicum and 11 wild tomato species using the AFLP method. According to the AFLP analysis, four combinations of primers E32/M59, E32/M57, E38/M57, and E41/M59, which had the highest PIC (polymorphism information content) values, were selected. In the process of genotyping a collection of 59 cultivars/lines of S. lycopersicum and 11 wild tomato accessions, the selected primers revealed 391 fragments ranging in size from 80 to 450 bp, of which 114 fragments turned out to be polymorphic and 25 were unique. Analysis of the amplification spectra placed wild tomato accessions into separate clades. Sister clades included cultivars of FSCV breeding resistant to drought and/or cold and, in part, to late blight, Alternaria, Septoria, tobacco mosaic virus and blossom end rot, as well as tomato accessions not characterized according to these traits, which suggests that they have resistance to stress factors. In accessions of distant clades, there was clustering on the basis of resistance to Verticillium, cladosporiosis, Fusarium, tobacco mosaic virus, gray rot, and blossom end rot. The combination of accessions according to their origin from the originating organization was shown. The primer combinations E32/M59, E32/M57, E38/M57 and E41/M59 were shown to be perspective for genotyping tomato cultivars to select donors of resistance to various stress factors. The clade-specific fragments identified in this work can become the basis for the development of AFLP markers for traits of resistance to stress factors.

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The authors declare that there are no conflicts of interest present.