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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJGB-22-82</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-3537</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>АКТУАЛЬНЫЕ МЕТОДЫ БИОТЕХНОЛОГИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>CURRENT BIOTECHNOLOGICAL METHODS</subject></subj-group></article-categories><title-group><article-title>Картирование локусов, ассоциированных с устойчивостью к полеганию у яровой мягкой пшеницы (Triticum aestivum L.)</article-title><trans-title-group xml:lang="en"><trans-title>Localization of the quantitative trait loci related to lodging resistance in spring bread wheat (Triticum aestivum L.)</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6516-0545</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Леонова</surname><given-names>И. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Leonova</surname><given-names>I. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><email xlink:type="simple">leonova@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7714-5609</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Агеева</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Ageeva</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Сибирский научно-исследовательский институт растениеводства и селекции – филиал Федерального исследовательского центра Институт цитологии и генетики Сибирского отделения Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Siberian Research Institute of Plant Production and Breeding – Branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>29</day><month>11</month><year>2022</year></pub-date><volume>26</volume><issue>7</issue><fpage>765</fpage><lpage>683</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Леонова И.Н., Агеева Е.В., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Леонова И.Н., Агеева Е.В.</copyright-holder><copyright-holder xml:lang="en">Leonova I.N., Ageeva E.V.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/3537">https://vavilov.elpub.ru/jour/article/view/3537</self-uri><abstract><p> Урожайность и качество зерна яровой и озимой пшеницы в значительной степени зависят от устойчивости сортов к полеганию. Генетический контроль устойчивости к полеганию носит количественный характер и контролируется большим числом генных локусов, поэтому изучение генетической архитектуры данного признака необходимо для создания и усовершенствования современных сортов. В работе представлены результаты по выявлению геномных районов, ассоциированных с устойчивостью к полеганию и сопряженными с ним признаками «высота растения» и «диаметр верхнего междоузлия» у российских сортов яровой мягкой пшеницы. Фенотипический скрининг 97 яровых сортов и селекционных линий мягкой пшеницы был проведен в полевых условиях Западно-Сибирского региона в 2017–2019 гг. Установлено, что 54 % сортообразцов можно охарактеризовать как средне- и высокоустойчивые к полеганию, при этом отмечено варьирование признака по годам. Двенадцать сортов проявляли низкий уровень устойчивости во все годы проведения испытаний. Группировка растений по высоте показала, что 19 образцов относятся к полукарликам (60–84 см), остальные вошли в группу низкорослых растений (85–100 см). Картирование локусов было проведено с помощью полногеномного ассоциативного (GWA) анализа с использованием 9285 маркеров SNP. Для признаков «устойчивость к полеганию», «высота растения» и «диаметр верхнего междоузлия» найдено 26 значимых ассоциаций (–logp&gt;3) в хромосомах 1B, 2A, 3A, 3D, 4A, 5A, 5B, 5D, 6A и 7B. Полученные результаты позволяют предположить, что районы 700–711 и 597–618 Mb хромосом 3А и 6А соответственно могут содержать кластеры генов, влияющих на устойчивость к полеганию и высоту растения. Не обнаружено районов хромосом с колокализацией локусов, ассоциированных с устойчивостью к полеганию и диаметром верхнего междоузлия. Данные GWA анализа могут иметь значение для разработки методов создания устойчивых к полеганию сортов с помощью маркер-ориентированной и геномной селекции.</p></abstract><trans-abstract xml:lang="en"><p>The yield and grain quality of spring and winter wheat significantly depends on varieties’ resistance to lodging, the genetic basis of this trait being quantitative and controlled by a large number of loci. Therefore, the study of the genetic architecture of the trait becomes necessary for the creation and improvement of modern wheat varieties. Here we present the results of localization of the genomic regions associated with resistance to lodging, plant height, and upper internode diameter in Russian bread wheat varieties. Phenotypic screening of 97 spring varieties and breeding lines was carried out in the field conditions of the West Siberian region during 2017–2019. It was found that 54 % of the varieties could be characterized as medium and highly resistant to lodging. At the same time, it was noted that the trait varied over the years. Twelve varieties showed a low level of resistance in all years of evaluation. Plant height-based grouping of the varieties showed that 19 samples belonged to semi-dwarfs (60–84 cm), and the rest were included in the group of standard-height plants (85–100 cm). Quantitative trait loci (QTL) mapping was performed by means of genome-wide association study (GWAS) using 9285 SNP markers. For lodging resistance, plant height, and upper internode diameter, 26 significant associations (–log p &gt; 3) were found in chromosomes 1B, 2A, 3A, 3D, 4A, 5A, 5B, 5D, 6A, and 7B. The results obtained suggest that the regions of 700–711 and 597–618 Mb in chromosomes 3A and 6A, respectively, may contain clusters of genes that affect lodging resistance and plant height. No chromosome regions colocalized with the QTLs associated with lodging resistance or upper internode diameter were found. The present GWAS results may be important for the development of approaches for creating lodging-resistant varieties through marker-assisted and genomic selection.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>яровая пшеница</kwd><kwd>полегание</kwd><kwd>высота растения</kwd><kwd>диаметр верхнего междоузлия</kwd><kwd>GWAS</kwd><kwd>QTL</kwd></kwd-group><kwd-group xml:lang="en"><kwd>spring wheat</kwd><kwd>lodging</kwd><kwd>plant height</kwd><kwd>upper internode diameter</kwd><kwd>GWAS</kwd><kwd>QTL</kwd></kwd-group><funding-group xml:lang="en"><funding-statement>This work was supported by a grant from the Russian Science Foundation (project No. 21-76-30003). 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