vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-16vavilov-3675Research ArticleГЕНЕТИЧЕСКИЕ РЕСУРСЫ И БИОКОЛЛЕКЦИИPLANT GENETICSМетаболомный подход в изучении Dactylis glomerata L. из коллекции ВИРMetabolomic approach to investigate Dactylis glomerata L. from the VIR collectionhttps://orcid.org/0000-0002-5688-6694МалышеваН. Ю.MalyshevaN. Yu.

Санкт-Петербург

l.malyshev@vir.nw.ruhttps://orcid.org/0000-0003-3992-5353ШеленгаТ. В.ShelengaT. V.

Санкт-Петербург

https://orcid.org/0000-0002-6201-4294СоловьеваА. Е.SolovyevaA. E.

Санкт-Петербург

https://orcid.org/0000-0003-4594-5799НагиевТ. Б.NagievT. B.

Ленинградская область

Leningrad region

https://orcid.org/0000-0002-9020-8336КовалеваН. В.KovalevaN. V.

Ленинградская область

Leningrad region

https://orcid.org/0000-0002-8595-1336МалышевЛ. Л.MalyshevL. L.

Санкт-Петербург

St. Petersburg

Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР)РоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian FederationЛенинградский научно-исследовательский институт сельского хозяйства Белогорка – филиал Федерального исследовательского центра картофеля имени А.Г. ЛорхаРоссияLeningrad Research Agriculture Institute Branch of Russian Potato Research CentreRussian Federation202306042023272111118Copyright © Малышева Н.Ю., Шеленга Т.В., Соловьева А.Е., Нагиев Т.Б., Ковалева Н.В., Малышев Л.Л., 20232023Малышева Н.Ю., Шеленга Т.В., Соловьева А.Е., Нагиев Т.Б., Ковалева Н.В., Малышев Л.Л.Malysheva N.Y., Shelenga T.V., Solovyeva A.E., Nagiev T.B., Kovaleva N.V., Malyshev L.L.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3675

Многолетний злак ежа сборная (Dactylis glomerata L.) – ценная ранняя высокопитательная культура, используемая в качестве зеленого корма в сельскохозяйственном производстве. Вид широко распространен на территории евразийского континента, характеризуется пластичностью и высокой эколого-географической изменчивостью. В статье рассмотрены метаболитные профили 15 образцов ежи сборной из коллекции Всероссийского института генетических ресурсов растений им. Н.И. Вавилова (ВИР). Материал представлен сортами и дикорастущими формами различного происхождения: европейская часть РФ, Норвегия и Финляндия. Исследование проводили с помощью газо-жидкостной хроматографии, сопряженной с масс-спектрометрией. Выполнено изучение и сравнение групп метаболитов образцов ежи сборной различного эколого-географического происхождения. Статистическая обработка включала вычисление основных параметров изменчивости, факторный анализ системы корреляций (Qи R-техника), кластерный анализ по методу Варда и дискриминантный анализ. Выявлена изменчивость количественного и качественного состава идентифицированных веществ. На основе статистической обработки полученных результатов выделили пять групп образцов ежи, различающихся по профилю метаболитов. В одной группе с похожим составом метаболитов оказались образцы из одного эколого-географического региона, в другой – образцы различного происхождения. Отмечены значительные отличия метаболомных профилей позднеспелого дикорастущего образца ежи из Карелии в фазе выхода в трубку от раннеи среднеспелых образцов в фазе колошения: он содержал наибольшее количество свободных аминокислот и наименьшее число выявленных первичных и вторичных метаболитов. Дикорастущий образец к-44020 из Норвегии в фазе колошения превзошел остальные дикорастущие образцы по содержанию свободных аминокислот, сахаров и фосфатов. Дикорастущие образцы отличались от селекционных сортов высоким содержанием пролина и треонина, устойчивостью к недостатку влаги и высокой температуре воздуха.

The perennial grass cocksfoot (Dactylis glomerata L.) is a valuable early highly nutritious crop used as green fodder in agricultural production. The species is widespread across the Eurasian continent; it is characterized by plasticity and high ecological and geographical variability. The article considers the metabolic profiles of 15 accessions of the cocksfoot from the collection of the N.I. Vavilov Institute of Plant Genetic Resources (VIR). The material is represented by varieties and wild forms of various origin: the European part of the Russian Federation, Norway and Finland. The study was carried out using gas-liquid chromatography coupled with mass spectrometry. The study and comparison of groups of metabolites of cocksfoot accessions of various ecological and geographical origin was carried out. Statistical processing included the calculation of the main parameters of variability, factor analysis of the correlation system (Qand R-technique), cluster analysis by Ward’s method and discriminant analysis. The variability of the quantitative and qualitative composition of the substances identified was revealed. Based on statistical processing of the results obtained, five groups of cocksfoot accessions were identified, differing in the profile of metabolites. One of the groups with a similar composition of metabolites consisted of accessions from one ecological and geographical region; another, of accessions of different origin. Significant differences were noted in the metabolomic profiles of a late-maturing wild cocksfoot accession from the Republic of Karelia at the booting stage from earlyand mid-maturing accessions at the heading stage; it contained the largest number of free amino acids and the smallest number of identified primary and secondary metabolites. Wild-growing accession k-44020 from Norway surpassed other wild-growing accessions in the content of free amino acids, sugars and phosphates at the heading stage. Wildgrowing accessions differed from breeding varieties with a high content of proline and threonine, indicators of high resistance to lack of moisture and high air temperature.

Dactylis glomerataгенетические ресурсыметаболомное профилированиеполиморфизм признаковDactylis glomeratagenetic resourcesmetabolomic profilingcharacter polymorphismReferencesBalmer D., Flors V., Glauser G., Mauch-Mani B. Metabolomics of cereals under biotic stress: current knowledge and techniques. Front. Plant Sci. 2013;4:82. DOI:10.3389/fpls.2013.00082.Balmer D., Flors V., Glauser G., Mauch-Mani B. Metabolomics of cereals under biotic stress: current knowledge and techniques. Front. Plant Sci. 2013;4:82. DOI:10.3389/fpls.2013.00082.Cañete-Rodríguez A.M., Santos-Duenas I.M., Jimenez-Hornero J.E., Ehrenreich A., Liebl W., Garcia-Garcia I. Gluconic acid: Properties, production methods and applications – an excellent opportunity for agroindustrial by-products and waste bio-valorization. Process Biochem. 2016;51(12):1891-1903. DOI:10.1016/j.procbio.2016.08.028.Cañete-Rodríguez A.M., Santos-Duenas I.M., Jimenez-Hornero J.E., Ehrenreich A., Liebl W., Garcia-Garcia I. Gluconic acid: Properties, production methods and applications – an excellent opportunity for agroindustrial by-products and waste bio-valorization. Process Biochem. 2016;51(12):1891-1903. DOI:10.1016/j.procbio.2016.08.028.Gallo M.B.C., Sarachine M.J. Biological activities of lupeol. Int. J. Res. Pharm. Biomed. Sci. 2009;3:46-66.Gallo M.B.C., Sarachine M.J. Biological activities of lupeol. Int. J. Res. Pharm. Biomed. Sci. 2009;3:46-66.Ibragimova S.S., Gorelova V.V., Kochetov A.V., Shumnyi V.K. The role of various metabolites in the formation of plant stress resistance. Vestnik Novosibirskogo Gosudarstvennogo Universiteta. Seriya: Biologiya, Klinicheskaya Meditsina = Bulletin of Novosibirsk State University. Series: Biology, Clinical Medicine. 2010;8(3):98-103. (in Russian)Ibragimova S.S., Gorelova V.V., Kochetov A.V., Shumnyi V.K. The role of various metabolites in the formation of plant stress resistance. Vestnik Novosibirskogo Gosudarstvennogo Universiteta. Seriya: Biologiya, Klinicheskaya Meditsina = Bulletin of Novosibirsk State University. Series: Biology, Clinical Medicine. 2010;8(3):98-103. (in Russian)Khan R.U., Naz S., Raziq F., Qudratullah Q., Khan N.A., Laudadio V., Tufarelli V., Ragni M. Prospects of organic acids as safe alternative to antibiotics in broiler chickens diet. Environ. Sci. Pollut. Res. 2022;29(22):32594-32604. DOI:10.1007/s11356-022-19241-8.Khan R.U., Naz S., Raziq F., Qudratullah Q., Khan N.A., Laudadio V., Tufarelli V., Ragni M. Prospects of organic acids as safe alternative to antibiotics in broiler chickens diet. Environ. Sci. Pollut. Res. 2022;29(22):32594-32604. DOI:10.1007/s11356-022-19241-8.Last L., Widmer F., Fjellstad W., Stoyanova S., Kölliker R. Genetic diversity of natural orchardgrass (Dactylis glomerata L.) populations in three regions in Europe. BMC Genet. 2013;14:102. DOI:10.1186/1471-2156-14-102.Last L., Widmer F., Fjellstad W., Stoyanova S., Kölliker R. Genetic diversity of natural orchardgrass (Dactylis glomerata L.) populations in three regions in Europe. BMC Genet. 2013;14:102. DOI:10.1186/1471-2156-14-102.Latypova G.M., Ivanova D.F., Davletshina R.Ya., Urazlina O.I. Composition of organic acids in plants of the genus primrose. Sibirskii Medicinskii Zhurnal = Siberian Medical Journal. 2014;3:96-98. (in Russian)Latypova G.M., Ivanova D.F., Davletshina R.Ya., Urazlina O.I. Composition of organic acids in plants of the genus primrose. Sibirskii Medicinskii Zhurnal = Siberian Medical Journal. 2014;3:96-98. (in Russian)Leiva A., Granados­Chinchilla F. Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. JAEFS. 2020;4(1):7-89. DOI:10.31015/jaefs.2020.9.Leiva A., Granados­Chinchilla F. Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. JAEFS. 2020;4(1):7-89. DOI:10.31015/jaefs.2020.9.Loskutov I., Shelenga T., Blinova E., Gnutikov A., Konarev A. Metabolomic profiling in evaluation of cultivated oat species with different ploidy level. BIO Web Conf. 2021;36:01026. DOI:10.1051/bioconf/20213601026.Loskutov I., Shelenga T., Blinova E., Gnutikov A., Konarev A. Metabolomic profiling in evaluation of cultivated oat species with different ploidy level. BIO Web Conf. 2021;36:01026. DOI:10.1051/bioconf/20213601026.Loskutov I.G., Shelenga T.V., Konarev A.V., Vargach Yu.Yu., Porokhovinova E.A., Blinova E.V., Gnutikov A.A., Rodionov A.V. A new approach to structuring the varietal diversity of naked and filmy forms of cultivated oats (Avena sativa L.). Ekologicheskaya Genetika = Ecological Genetics. 2020;18(1):27-41. DOI:10.17816/ecogen 12977. (in Russian)Loskutov I.G., Shelenga T.V., Konarev A.V., Vargach Yu.Yu., Porokhovinova E.A., Blinova E.V., Gnutikov A.A., Rodionov A.V. A new approach to structuring the varietal diversity of naked and filmy forms of cultivated oats (Avena sativa L.). Ekologicheskaya Genetika = Ecological Genetics. 2020;18(1):27-41. DOI:10.17816/ecogen 12977. (in Russian)Lotova L.I. Botany. Morphology and anatomy of higher plants. Moscow: KomKniga Publ., 2007. (in Russian)Lotova L.I. Botany. Morphology and anatomy of higher plants. Moscow: KomKniga Publ., 2007. (in Russian)Mahfuz S., Shang Q., Piao X. Phenolic compounds as natural feed additives in poultry and swine diets: a review. J. Animal Sci. Biotechnol. 2021;12:48. DOI:10.1186/s40104-021-00565-3.Mahfuz S., Shang Q., Piao X. Phenolic compounds as natural feed additives in poultry and swine diets: a review. J. Animal Sci. Biotechnol. 2021;12:48. DOI:10.1186/s40104-021-00565-3.Mandrone M., Marincich L., Chiocchio I., Zannini P., Guarino R., Poli F. Metabolomic study of Dactylis glomerata growing on Aeolian archipelago (Italy). Metabolites. 2022;12(6):533. DOI:10.3390/metabo12060533.Mandrone M., Marincich L., Chiocchio I., Zannini P., Guarino R., Poli F. Metabolomic study of Dactylis glomerata growing on Aeolian archipelago (Italy). Metabolites. 2022;12(6):533. DOI:10.3390/metabo12060533.Maslennikov P.V., Chupakhina G.N., Skrypnik L.N. Content of phenolic compounds in medicinal plants of the Botanical garden. Izvestiya RAN Biologicheskaya seria = Proceedings of Russian Academy of Sciences. Biological Series. 2013;5:551-557. DOI:10.7868/s000233291305010x. (in Russian)Maslennikov P.V., Chupakhina G.N., Skrypnik L.N. Content of phenolic compounds in medicinal plants of the Botanical garden. Izvestiya RAN Biologicheskaya seria = Proceedings of Russian Academy of Sciences. Biological Series. 2013;5:551-557. DOI:10.7868/s000233291305010x. (in Russian)Maslennikov P.V., Chupakhina G.N., Skrypnik L.N., Mal’tseva E.Yu., Poltavskaya R.L. The content of low-molecular antioxidants in medicinal plants of the Kaliningrad region. Khimiya Rastitel’nogo Syr’ya = Chemistry of Plant Stock. 2012;3:127-133. (in Russian)Maslennikov P.V., Chupakhina G.N., Skrypnik L.N., Mal’tseva E.Yu., Poltavskaya R.L. The content of low-molecular antioxidants in medicinal plants of the Kaliningrad region. Khimiya Rastitel’nogo Syr’ya = Chemistry of Plant Stock. 2012;3:127-133. (in Russian)Matveeva T.V., Sokornova S.V. Biological traits of naturally transgenic plants and their evolutional roles. Fiziologiya Rastenii = Russian Journal of Plant Physiology. 2017;64(5):323-336. DOI:10.1134/S1021443717050089. (in Russian)Matveeva T.V., Sokornova S.V. Biological traits of naturally transgenic plants and their evolutional roles. Fiziologiya Rastenii = Russian Journal of Plant Physiology. 2017;64(5):323-336. DOI:10.1134/S1021443717050089. (in Russian)Misin V.M., Sazhina N.N., Zav’yalov A.Yu. Seasonal dynamics of changes in the content of phenolic antioxidants in plantain and dandelion leaves. Khimiya Rastitel’nogo Syr’ya = Chemistry of Plant Stock. 2010;3:103-106. (in Russian)Misin V.M., Sazhina N.N., Zav’yalov A.Yu. Seasonal dynamics of changes in the content of phenolic antioxidants in plantain and dandelion leaves. Khimiya Rastitel’nogo Syr’ya = Chemistry of Plant Stock. 2010;3:103-106. (in Russian)Noiraud N., Maurousset L., Lemoine R. Transport of polyols in higher plants. Plant Physiol. Biochem. 2001;39(9):717-728. DOI:10.1016/S0981-9428(01)01292-X.Noiraud N., Maurousset L., Lemoine R. Transport of polyols in higher plants. Plant Physiol. Biochem. 2001;39(9):717-728. DOI:10.1016/S0981-9428(01)01292-X.Novikov N.N. Biochemistry of plants. Moscow: KolosS Publ., 2012. (in Russian)Novikov N.N. Biochemistry of plants. Moscow: KolosS Publ., 2012. (in Russian)Pandyan M., Subramanian R.K., Subramani P., Narayanan M., Wilson A., Shunmuga K.P., Manikandan R. Global analysis of threonine metabolism genes unravel key players in rice to improving the abiotic stress tolerance. Sci. Rep. 2018;8:9270. DOI:10.1038/s41598018-27703-8.Pandyan M., Subramanian R.K., Subramani P., Narayanan M., Wilson A., Shunmuga K.P., Manikandan R. Global analysis of threonine metabolism genes unravel key players in rice to improving the abiotic stress tolerance. Sci. Rep. 2018;8:9270. DOI:10.1038/s41598018-27703-8.Rasmussen S., Parsons A.J., Jones C.S. Metabolomics of forage plants: a review. Ann. Bot. 2012;110(6):1281-12890. DOI:10.1093/aob/mcs023.Rasmussen S., Parsons A.J., Jones C.S. Metabolomics of forage plants: a review. Ann. Bot. 2012;110(6):1281-12890. DOI:10.1093/aob/mcs023.Sazhina N.N., Misin V.M. Measurement of the total content of phenolic compounds in various parts of medicinal plants. Khimiya Rastitel’nogo Syr’ya = Chemistry of Plant Stock. 2011;3:149-152. (in Russian)Sazhina N.N., Misin V.M. Measurement of the total content of phenolic compounds in various parts of medicinal plants. Khimiya Rastitel’nogo Syr’ya = Chemistry of Plant Stock. 2011;3:149-152. (in Russian)Sampieva K.T., Oganova G.M., Ivashev M.N., Chuklin R.E., Guseinov A.K. Study of the effects of certain amino acids in hypoxic hypoxia. Biomeditsina = Biomedicine. 2010;4:122-123. (in Russian)Sampieva K.T., Oganova G.M., Ivashev M.N., Chuklin R.E., Guseinov A.K. Study of the effects of certain amino acids in hypoxic hypoxia. Biomeditsina = Biomedicine. 2010;4:122-123. (in Russian)Shelenga T.V., Solov’eva A.E., Shevarda A.L., Konarev A.V. Research of the VIR collection cultures metabolome. In: Abstracts of the international scientific conference ‘Plant genetic resources – the basis for food security and improving the quality of life’ dedicated to the 120th anniversary of VIR. October 6–8, 2014. St. Petersburg: VIR, 2014;98. (in Russian)Shelenga T.V., Solov’eva A.E., Shevarda A.L., Konarev A.V. Research of the VIR collection cultures metabolome. In: Abstracts of the international scientific conference ‘Plant genetic resources – the basis for food security and improving the quality of life’ dedicated to the 120th anniversary of VIR. October 6–8, 2014. St. Petersburg: VIR, 2014;98. (in Russian)Shilova I.V., Baranovskaya N.V., Syrchina A.I., Baranova O.V., Dudko V.V., Semenov A.A., Suslov N.I. Amino acid and elemental composition of the active fraction of the Siberian clematis. Voprosy Biologichekoy, Medicinskoy i Farmatsevticheskoy Khimii = Issue of Biological, Medical and Pharmaceutical Chemistry. 2008;3:34-37. (in Russian)Shilova I.V., Baranovskaya N.V., Syrchina A.I., Baranova O.V., Dudko V.V., Semenov A.A., Suslov N.I. Amino acid and elemental composition of the active fraction of the Siberian clematis. Voprosy Biologichekoy, Medicinskoy i Farmatsevticheskoy Khimii = Issue of Biological, Medical and Pharmaceutical Chemistry. 2008;3:34-37. (in Russian)Shkrobot’ko P.Yu., Popov D.M., Fursa N.S. Amino acid composition of underground organs of Fory’s valerian and elder leaf valerian. Farmatsiya = Farmacia. 2009;7:19-23. (in Russian)Shkrobot’ko P.Yu., Popov D.M., Fursa N.S. Amino acid composition of underground organs of Fory’s valerian and elder leaf valerian. Farmatsiya = Farmacia. 2009;7:19-23. (in Russian)Shurson G.C., Kerr B.J., Hanson A.R. Evaluating the quality of feed fats and oils and their effects on pig growth performance. J. Animal Sci. Biotechnol. 2015;6:10. DOI:10.1186/s40104-015-0005-4.Shurson G.C., Kerr B.J., Hanson A.R. Evaluating the quality of feed fats and oils and their effects on pig growth performance. J. Animal Sci. Biotechnol. 2015;6:10. DOI:10.1186/s40104-015-0005-4.Solovyeva A.E., Shelenga T.V., Shavarda A.L., Burlyaeva M.O. Сomparative analysis of wild and cultivated Lathyrus L. spp. according to their primary and secondary metabolite contents. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2019;23(6):667-674. DOI:10.18699/VJ19.539.Solovyeva A.E., Shelenga T.V., Shavarda A.L., Burlyaeva M.O. Сomparative analysis of wild and cultivated Lathyrus L. spp. according to their primary and secondary metabolite contents. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2019;23(6):667-674. DOI:10.18699/VJ19.539.Solovyeva A.E., Shelenga T.V., Shavarda A.L., Burlyaeva M.O. Comparative analysis of wild and cultivated Lathyrus L. species to assess their content of sugars, polyols, free fatty acids, and phytosterols. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2020;24(7):730-737. DOI:10.18699/VJ20.667.Solovyeva A.E., Shelenga T.V., Shavarda A.L., Burlyaeva M.O. Comparative analysis of wild and cultivated Lathyrus L. species to assess their content of sugars, polyols, free fatty acids, and phytosterols. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2020;24(7):730-737. DOI:10.18699/VJ20.667.Stewart A.V., Ellison N.W. Dactylis. In: Kole C. (Ed.). Wild crop relatives: Genomic and breeding resources. Millets and grasses. Heidelberg: Springer: 2011;73-87. DOI:10.1007/978-3-642-14255-0_5.Stewart A.V., Ellison N.W. Dactylis. In: Kole C. (Ed.). Wild crop relatives: Genomic and breeding resources. Millets and grasses. Heidelberg: Springer: 2011;73-87. DOI:10.1007/978-3-642-14255-0_5.Tulinov A.G., Kosolapova T.V., Mikhailova E.A. Results of the evaluation of collection accessions of Dactylis glomerata L. in conditions of republic Komi. Zemledelie = Husbandry. 2019;3:41-43. DOI:10.24411/0044-3913-2019-10311. (in Russian)Tulinov A.G., Kosolapova T.V., Mikhailova E.A. Results of the evaluation of collection accessions of Dactylis glomerata L. in conditions of republic Komi. Zemledelie = Husbandry. 2019;3:41-43. DOI:10.24411/0044-3913-2019-10311. (in Russian)Yakovleva L.V., Kalashnik M.V., Zaika I.B., Gracheva L.S., Fesen ko M.A., Evdokimova Z.Z., Gadzhiev N.M., Bekish L.P., Ivano va N.V., Pozdnyakov V.A., Andrushchenko A.V. Catalogue of forage crop varieties breeding in Leningrad Research Agriculture Institute Branch of Russian Potato Research Centre. St-Petersburg: Leningrad Research Agriculture Institute Publ., 2015. (in Russian)Yakovleva L.V., Kalashnik M.V., Zaika I.B., Gracheva L.S., Fesen ko M.A., Evdokimova Z.Z., Gadzhiev N.M., Bekish L.P., Ivano va N.V., Pozdnyakov V.A., Andrushchenko A.V. Catalogue of forage crop varieties breeding in Leningrad Research Agriculture Institute Branch of Russian Potato Research Centre. St-Petersburg: Leningrad Research Agriculture Institute Publ., 2015. (in Russian)

The authors declare that there are no conflicts of interest present.