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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJGB-23-18</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-3677</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>СЕЛЕКЦИЯ РАСТЕНИЙ НА ИММУНИТЕТ И ПРОДУКТИВНОСТЬ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT BREEDING FOR IMMUNITY AND PERFORMANCE</subject></subj-group></article-categories><title-group><article-title>ДНК-маркерная идентификация локуса устойчивости к милдью Rpv10 в генотипах винограда</article-title><trans-title-group xml:lang="en"><trans-title>DNA marker identification of downy mildew resistance locus Rpv10 in grapevine genotypes</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2446-0971</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ильницкая</surname><given-names>Е. Т.</given-names></name><name name-style="western" xml:lang="en"><surname>Ilnitskaya</surname><given-names>E. T.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Краснодар</p></bio><bio xml:lang="en"><p>Krasnodar</p></bio><email xlink:type="simple">ilnitskaya79@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3397-0666</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Макаркина</surname><given-names>М. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Makarkina</surname><given-names>M. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Краснодар</p></bio><bio xml:lang="en"><p>Krasnodar</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2092-7757</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Токмаков</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Toкmakov</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Краснодар</p></bio><bio xml:lang="en"><p>Krasnodar</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5051-2616</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Наумова</surname><given-names>Л. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Naumova</surname><given-names>L. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новочеркасск</p></bio><bio xml:lang="en"><p>Novocherkassk</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Северо-Кавказский федеральный научный центр садоводства, виноградарства, виноделия<country>Россия</country></aff><aff xml:lang="en">North-Caucasian Federal Scientific Center of Horticulture, Viticulture, Winemaking<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Всероссийский научно-исследовательский институт виноградарства и виноделия имени Я.И. Потапенко – филиал Федерального Ростовского аграрного научного центра<country>Россия</country></aff><aff xml:lang="en">Ya.I. Potapenko All-Russian Research Institute of Viticulture and Winemaking – branch of Federal Rostov Agricultural Research Center<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>06</day><month>04</month><year>2023</year></pub-date><volume>27</volume><issue>2</issue><fpage>129</fpage><lpage>134</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Ильницкая Е.Т., Макаркина М.В., Токмаков С.В., Наумова Л.Г., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Ильницкая Е.Т., Макаркина М.В., Токмаков С.В., Наумова Л.Г.</copyright-holder><copyright-holder xml:lang="en">Ilnitskaya E.T., Makarkina M.V., Toкmakov S.V., Naumova L.G.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/3677">https://vavilov.elpub.ru/jour/article/view/3677</self-uri><abstract><p>Милдью – одно из наиболее распространенных и вредоносных заболеваний виноградной лозы, возбудителем которого считают Plasmopara viticola. Сорта Vitis vinifera, выступая основой высококачественного виноградарства, практически не обладают генетической устойчивостью к милдью. Генотипы, имеющие природную устойчивость к поражению P. viticola, принадлежат видам винограда Северной Америки и Азии (V. aestivalis, V. berlandieri, V. cinerea, V. labrusca, V. amurensis и др.), а также к Muscadinia rotundifolia. По этой причине создание сортов винограда с повышенной устойчивостью к патогену основано на межвидовой гибридизации. В настоящее время молекулярногенетические методы анализа все активнее используют на этапах предселекционной работы и непосредственно в селекции. Один из крупных локусов устойчивости к милдью – ген Rpv10 – впервые идентифицирован в сорте межвидового происхождения Солярис и изначально происходит от дикого амурского винограда. Известны ДНК-маркеры данного гена, позволяющие детектировать наличие Rpv10 в генотипах винограда. Методом ПЦР-анализа выполнен поиск доноров гена устойчивости среди генотипов 30 сортов винограда, которые, согласно родословным, могли бы нести ген Rpv10. Работа выполнена с использованием автоматического генетического анализатора, что позволяет получать высокоточные данные. По результатам ДНК-маркерного анализа в 10 генотипах винограда выявлено наличие аллели гена Rpv10, определяющей устойчивость к возбудителю милдью. Выполнено генотипирование сортов винограда, в которых обнаружен Rpv10, с помощью шести стандартных для ДНК-профилирования винограда SSR-маркеров. ДНК-маркерный анализ показал наличие аллели устойчивости у сорта Коринка русская, который, по общедоступным данным, является потомком сорта Заря Севера, не обладающим геном устойчивости Rpv10. С использованием анализа полиморфизма микросателлитных локусов и базы данных VIVC уточнена родословная сорта винограда Коринка русская. Установлено, что Коринка русская происходит от сорта Северный – донора локуса устойчивости Rpv10.</p></abstract><trans-abstract xml:lang="en"><p>One of the most common and harmful diseases of grapevine is downy mildew, caused by Plasmopara viticola. Cultivars of Vitis vinifera, the basis of high-quality viticulture, are mainly not resistant to downy mildew. Varieties with natural resistance to downy mildew belong to the vine species of North America and Asia (V. aestivalis, V. berlandieri, V. cinerea, V. labrusca, V. amurensis, etc.), as well as Muscadinia rotundifolia. The breeding of resistant cultivars is based on interspecific crossing. Currently, molecular genetic methods are increasingly used in pre-selection work and directly in breeding. One of the major loci of downy mildew resistance, Rpv10, was first identified in the variety Solaris and was originally inherited from wild V. amurensis. DNA markers that allow detecting Rpv10 in grapevine genotypes are known. We used PCR analysis to search for donors of resistance locus among 30 grape cultivars that, according to their pedigrees, could carry Rpv10. The work was performed using an automatic genetic analyzer, which allows obtaining high-precision data. Rpv10 locus allele, which determines resistance to the downy mildew pathogen, has been detected in 10 genotypes. Fingerprinting of grape cultivars with detected Rpv10 was performed at 6 reference SSR loci. DNA marker analysis revealed the presence of a resistance allele in the cultivar Korinka russkaya, which, according to publicly available data, is the offspring of the cultivar Zarya Severa and cannot carry Rpv10. Using the microsatellite loci polymorphism analysis and the data from VIVC database, it was found that Korinka russkaya is the progeny of the cultivar Severnyi, which is the donor of the resistance locus Rpv10. The pedigree of the grapevine cultivar Korinka russkaya was also clarified.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Vitis sp.</kwd><kwd>целевые аллели</kwd><kwd>Plasmopara viticola</kwd><kwd>ДНК-профилирование</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Vitis sp.</kwd><kwd>target alleles</kwd><kwd>Plasmopara viticola</kwd><kwd>DNA fingerprinting</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Abuzov M. Atlas of Northern Grapes. Smolensk: KFH Pitomnik Publ., 2009. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Abuzov M. Atlas of Northern Grapes. Smolensk: KFH Pitomnik Publ., 2009. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Alleweldt G., Possingham J.V. Progress in grapevine breeding. Theor. Appl. Genet. 1988;75:669-673. 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