vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-20vavilov-3679Research ArticleФИЛОГЕНЕТИКАPHYLOGENETICSМитохондриальный геном Dendrobaena tellermanica Perel, 1966 (Annelida: Lumbricidae)The mitochondrial genome of Dendrobaena tellermanica Perel, 1966 (Annelida: Lumbricidae) and its phylogenetic positionhttps://orcid.org/0000-0001-5604-5601ШеховцовС. В.ShekhovtsovS. V.

Новосибирск, Магадан

Novosibirsk, Magadan

ВасильевГ. В.VasilievG. V.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-7201-5426ЛатифР.LatifR.

Семнан

Semnan

https://orcid.org/0000-0002-5652-0553ПолубояроваТ. В.PoluboyarovaT. V.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-3524-0456ПельтекС. Е.PeltekS. E.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-6766-1482РапопортИ. Б.RapoportI. B.

Нальчик

Nalchik

Федеральный исследовательский центр Институт цитологии и генетики, Сибирское отделение Российской академии наук; Институт биологических проблем Севера, Дальневосточное отделение Российской академии наукРоссияInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Institute of Biological Problems, North of the Far Eastern Branch of the Russian Academy of SciencesRussian FederationФедеральный исследовательский центр Институт цитологии и генетики, Сибирское отделение Российской академии наукРоссияInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Institute of Biological Problems, North of the Far Eastern Branch of the Russian Academy of SciencesRussian FederationУниверситет СемнанаИранSemnan UniversityIslamic Republic of IranФедеральный исследовательский центр Институт цитологии и генетики, Сибирское отделение Российской академии наукРоссияInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of SciencesRussian FederationИнститут экологии горных территорий им. А.К. Темботова, Российская академия наукРоссияTembotov Institute of Ecology of Mountain Territories, Russian Academy of SciencesRussian Federation202306042023272146152Copyright © Шеховцов С.В., Васильев Г.В., Латиф Р., Полубоярова Т.В., Пельтек С.Е., Рапопорт И.Б., 20232023Шеховцов С.В., Васильев Г.В., Латиф Р., Полубоярова Т.В., Пельтек С.Е., Рапопорт И.Б.Shekhovtsov S.V., Vasiliev G.V., Latif R., Poluboyarova T.V., Peltek S.E., Rapoport I.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3679

Дождевые черви – важная экологическая группа, которая оказывает значительное влияние как на состав почвенной фауны, так и на растительность. Генетическое разнообразие и филогения дождевых червей при этом остаются относительно слабо изученными. В настоящее время большинство работ по генетическому разнообразию дождевых червей основывается на единичных митохондриальных и ядерных генах. В связи с этим для реконструкции филогенетических отношений у дождевых червей перспективными становятся митохондриальные геномы. Почти все работы по этой теме посвящены видам из Западной Европы или Восточной Азии, другие регионы практически не затронуты. В настоящей работе мы провели секвенирование, сборку и анализ митохондриального генома Dendrobaena tellermanica Perel, 1966. Этот вид ранее входил в состав кавказского вида D. schmidti (Michaelsen, 1907) – политипического вида, в пределах которого выделяли множество таксонов. Геном был собран в виде одного контига длиной 15 298 п. н., содержащего типичный набор генов: 13 белок-кодирующих генов (три субъединицы цитохромоксидазы, семь субъединиц NADH дегидрогеназы, две субъединицы АТФ синтетазы, цитохром b), гены 12S и 16S рибосомальной РНК и 22 гена тРНК. Все гены были расположены на одной цепи ДНК. На контрольный регион, находящийся между генами tRNA-Arg и tRNA-His, приходилось 727 п. н. Контрольный регион содержал множество шпилек, а также тандемные повторы мономера AACGCTT. Филогенетический анализ на основе полных митохондриальных геномов показал, что род Dendrobaena является базальным в семействе Lumbricidae. D. tellermanica оказалась довольно далеким родственником космополитного вида D. octaedra, что говорит о высоком генетическом разнообразии в этом роде. Вид D. schmidti был парафилетичным по отношению к D. tellermanica. Поскольку для D. schmidti характерна очень высокая генетическая изменчивость, можно рассматривать эти данные как свидетельство в пользу разделения D. schmidti на несколько видов.

Earthworms are an important ecological group that has a significant impact on soil fauna as well as plant communities. Despite their importance, genetic diversity and phylogeny of earthworms are still insufficiently studied. Most studies on earthworm genetic diversity are currently based on a few mitochondrial and nuclear genes. Mitochondrial genomes are becoming a promising target for phylogeny reconstruction in earthworms. However, most studies on earthworm mitochondrial genomes were made on West European and East Asian species, with much less sampling from other regions. In this study, we performed sequencing, assembly, and analysis of the mitochondrial genome of Dendrobaena tellermanica Perel, 1966 from the Northern Caucasus. This species was earlier included into D. schmidti (Michaelsen, 1907), a polytypic species with many subspecies. The genome was assembled as a single contig 15,298 bp long which contained a typical gene set: 13 protein-coding genes (three subunits of cytochrome c oxidase, seven subunits of NADH dehydrogenase, two subunits of ATP synthetase, and cytochrome b), 12S and 16S ribosomal RNA genes, and 22 tRNA genes. All genes were located on one DNA strand. The assembled part of the control region, located between the tRNA-Arg and tRNA-His genes, was 727 bp long. The control region contained multiple hairpins, as well as tandem repeats of the AACGCTT monomer. Phylogenetic analysis based on the complete mitochondrial genomes indicated that the genus Dendrobaena occupied the basal position within Lumbricidae. D. tellermanica was a rather distant relative of the cosmopolitan D. octaedra, suggesting high genetic diversity in this genus. D. schmidti turned out to be paraphyletic with respect to D. tellermanica. Since D. schmidti is known to contain very high genetic diversity, these results may indicate that it may be split into several species.

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The authors declare that there are no conflicts of interest present.