vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-21vavilov-3680Research ArticleФИЛОГЕНЕТИКАPHYLOGENETICSИспользование первичной структуры района ITS1–ITS2 для видовой идентификации у некоторых представителей водных макрофитов рода StuckeniaThe use of the primary structure of the ITS1–ITS2 region for species identification in some submerged aquatic macrophytes of the genus Stuckeniahttps://orcid.org/0000-0001-6040-6801МглинецА. В.MglinetsA. V.

Новосибирск

Novosibirsk

КостеринО. Э.KosterinО. E.

Новосибирск

Novosibirsk

kosterin@bionet.nsc.ruФедеральный исследовательский центр Институт цитологии и генетики, Сибирское отделение Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian Federation202306042023272153161Copyright © Мглинец А.В., Костерин О.Э., 20232023Мглинец А.В., Костерин О.Э.Mglinets A.V., Kosterin О.E.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3680

Проведен биоинформационный анализ первичной структуры района ITS1–ITS2 образцов S. vaginata и S. pectinata, взятых из публичных баз данных, ссылки на которые приведены в опубликованных работах. Показано, что межвидовые различия S. vaginata и S. pectinata обусловлены делециями/вставками и одноили двунуклеотидными заменами. Более того, вид S. pectinata по структуре района ITS1–ITS2 представлен двумя генотипами, которые обозначены как S. pectinata тип A и S. pectinata тип B, различия между которыми обусловлены одноили двунуклеотидными заменами. Это демонстрирует возможность применения данного района для определения видовой принадлежности у представителей рода Stuckenia. Для экспериментальной проверки возможности использования данного района у 28 образцов, определенных на основании морфологиче ских признаков как S. pectinata, S. vaginata, S. macrocarpa и S. chakassiensis, выполнено определение первичной структуры района ITS1–ITS2. Анализ полученных экспериментальных данных показал, что они распадаются на три группы, третья группа представлена двумя подгруппами. Эти группы соответствуют S. vaginata, S. pectinata тип A и S. pectinata тип B. В группу S. vaginata попали образцы, которые на основании морфологических признаков определены как S. vaginata. В группу S. pectinata тип A попали образцы, которые на основании морфологических признаков определены как S. pectinata. Группу S. pectinata тип B на основании первичной структуры района ITS1–ITS2 можно разделить на две подгруппы: S. pectinata тип B и S. chakassiensis. В подгруппу S. pectinata тип B вошли образцы, которые на основании морфологических признаков определены как S. pectinata, S. vaginata и S. macrocarpa. В подгруппу P. chakassiensis в основном вошли образцы, которые на основании морфологических признаков определены как S. chakassiensis. На основании выравнивания последовательностей подгруппы S. pectinata тип B сделано предположение, согласно которому в данную подгруппу объединены последовательности, принадлежащие S. pectinata тип B, S. macrocarpa и их гибридам.

Applicability of ITS1–ITS2 primary structure for species attribution of representatives of the genus Stuckenia was experimentally tested. Analysis of the ITS1–ITS2 region sequences of S. vaginata and S. pectinata from public databases showed that they differed by insertions/deletions and single or double nucleotide substitutions. Besides, the ITS1–ITS2 region of S. pectinata was shown to be represented by two haplotype groups designated as S. pectinata type A and S. pectinata type B with good bootstrap support in phylogenetic reconstructions. In 28 samples identified as S. pectinata, S. vaginata, S. macrocarpa and S. chakassiensis on the basis of morphology, the ITS1–ITS2 region was sequenced in this study. Three groups of samples with good bootstrap support were revealed to be corresponding to S. vaginata, S. pectinata type A and S. pectinata type B. The S. vaginata group was formed by the samples identified on the basis of morphology as S. vaginata, and the S. pectinata type A group was formed by the samples identified on the basis of morphology as S. pectinata. The S. pectinata type B group was further divided into two subgroups, S. pectinata type B subgroup and S. chakassiensis subgroup. The S. chakassiensis subgroup included mainly the samples identified as such on the basis of morphology. The S. pectinata type B subgroup included samples identified on the basis of morphology as S. pectinata, S. vaginata and S. macrocarpa. We suppose that these samples were S. pectinata type B, S. macrocarpa and their hybrids.

PotamogetonaceaeStuckeniaS. chakassiensisS. macrocarpaS. pectinataS. vaginataрайон ITS1–ITS2идентификация видовPotamogetonaceaeStuckeniaS. chakassiensisS. macrocarpaS. pectinataS. vaginataITSI–ITS2 regionspices identificationThe authors are grateful to L.M. Kipriyanova for providing plant material and to V.S. Bodganova for the help with English. This work was supported by the grant of Russian Foundation for the Fundamental Research (RFFI) project No. 13-04-02055-a and the scientific project FWNR-2022-0017ReferencesCoffey W.W. The feasibility of submerged macrophyte re­establishment in Kaituna Lagoon, Lake Ellesmere (Te Waihora). A thesis submitted in partial fulfilment of the requirements for the degree of Master of Applied Science in Resource Management/Ecology at Lincoln University. Lincoln University, N.Z. 2001. https://researcharchive. lincoln.ac.nz/handle/10182/2271Coffey W.W. The feasibility of submerged macrophyte re­establishment in Kaituna Lagoon, Lake Ellesmere (Te Waihora). A thesis submitted in partial fulfilment of the requirements for the degree of Master of Applied Science in Resource Management/Ecology at Lincoln University. Lincoln University, N.Z. 2001. https://researcharchive. lincoln.ac.nz/handle/10182/2271Desalle R. Species discovery versus species identification in DNA barcoding efforts: response to Rubinoff. Conserv. Biol. 2006;20(5): 1545-1547. DOI:10.1111/j.1523­1739.2006.00543.x.Desalle R. Species discovery versus species identification in DNA barcoding efforts: response to Rubinoff. Conserv. Biol. 2006;20(5): 1545-1547. DOI:10.1111/j.1523­1739.2006.00543.x.Fant J.B., Kamau E., Preston C.D. Chloroplast evidence for the multiple origins of the hybrid Potamogeton x sudermanicus Hagstr. Aquat. Bot. 2003;75(4):351-356. DOI:10.1016/S0304­3770(03)00004­4.Fant J.B., Kamau E., Preston C.D. Chloroplast evidence for the multiple origins of the hybrid Potamogeton x sudermanicus Hagstr. Aquat. Bot. 2003;75(4):351-356. DOI:10.1016/S0304­3770(03)00004­4.Fant J.B., Kamau E., Preston C.D. Chloroplast evidence for the multiple origins of the hybrid Potamogeton × fluitans. Aquat. Bot. 2005; 83(2):154-160. DOI:10.1016/j.aquabot.2005.06.004.Fant J.B., Kamau E., Preston C.D. Chloroplast evidence for the multiple origins of the hybrid Potamogeton × fluitans. Aquat. Bot. 2005; 83(2):154-160. DOI:10.1016/j.aquabot.2005.06.004.Fazekas A.J., Kuzmina M.L., Newmaster S.G., Hollingsworth P.M. DNA barcoding methods for land plants. Methods Mol. Biol. 2012; 858:223-252. DOI:10.1007/978­1­61779­591­6_11.Fazekas A.J., Kuzmina M.L., Newmaster S.G., Hollingsworth P.M. DNA barcoding methods for land plants. Methods Mol. Biol. 2012; 858:223-252. DOI:10.1007/978­1­61779­591­6_11.Guseva N.V., Kopylova Yu.G., Khvashchevskaya A.A., Smetanina I.V. Chemical content of salt lakes of the Severo-Minusinsk depression, Khakasiya. Bull. Tomsk Polytech. Univ. 2012;321(1):163-168. (in Russian)Guseva N.V., Kopylova Yu.G., Khvashchevskaya A.A., Smetanina I.V. Chemical content of salt lakes of the Severo-Minusinsk depression, Khakasiya. Bull. Tomsk Polytech. Univ. 2012;321(1):163-168. (in Russian)Haynes R.R., Les D.H., Kral M. Two new combinations in Stuckenia, the correct name for Coleogeton (Potamogetonaceae). Novon. 1998a;8(3):241. DOI:10.2307/3392010.Haynes R.R., Les D.H., Kral M. Two new combinations in Stuckenia, the correct name for Coleogeton (Potamogetonaceae). Novon. 1998a;8(3):241. DOI:10.2307/3392010.Haynes R.R., Les D.H., Holm-Nielsen L.B.I. Potamogetonaceae. In: Kubitzki K. (Ed.). The families and genera of vascular plants, Flowering Plants: Monocotyledons: Alismatanae and CommelinanaeHaynes R.R., Les D.H., Holm-Nielsen L.B.I. Potamogetonaceae. In: Kubitzki K. (Ed.). The families and genera of vascular plants, Flowering Plants: Monocotyledons: Alismatanae and Commelinanae(except Gramineae). Vol. IV. Berlin: Springer­Verlag, 1998b;408­414. DOI:10.1007/978­3­662­03531­3_39.(except Gramineae). Vol. IV. Berlin: Springer­Verlag, 1998b;408­414. DOI:10.1007/978­3­662­03531­3_39.Hollingsworth P.M., Preston C.D., Gornall R.J. Euploid and aneuploid evolution in Potamogeton (Potamogetonaceae): a factual basis for interpretation. Aquat. Bot. 1998;60(4):337­358. DOI:10.1016/S0304-3770(97)00101-0.Hollingsworth P.M., Preston C.D., Gornall R.J. Euploid and aneuploid evolution in Potamogeton (Potamogetonaceae): a factual basis for interpretation. Aquat. Bot. 1998;60(4):337­358. DOI:10.1016/S0304-3770(97)00101-0.Hollingsworth P.M., Preston C.D., Gornall R.J. Isozyme evidence for the parentage and multiple origins of Potamogeton × suecicus (P. pectinatus × P. filiformis, Potamogetonaceae). Plant Syst. Evol. 1996;202(3):219-232.Hollingsworth P.M., Preston C.D., Gornall R.J. Isozyme evidence for the parentage and multiple origins of Potamogeton × suecicus (P. pectinatus × P. filiformis, Potamogetonaceae). Plant Syst. Evol. 1996;202(3):219-232.Holub J. Stuckenia Börner 1912 – the correct name for Coleogeton (Potamogetonaceae). Preslia. 1997;69,361-366.Holub J. Stuckenia Börner 1912 – the correct name for Coleogeton (Potamogetonaceae). Preslia. 1997;69,361-366.Iida S., Kosuge K., Kadono Y. Molecular phylogeny of Japanese Potamogeton species in light of noncoding chloroplast sequences. Aquat. Bot. 2004;80(2):115-127. DOI:10.1016/j.aquabot.2004.08.005.Iida S., Kosuge K., Kadono Y. Molecular phylogeny of Japanese Potamogeton species in light of noncoding chloroplast sequences. Aquat. Bot. 2004;80(2):115-127. DOI:10.1016/j.aquabot.2004.08.005.Ivanova Ye.A., Anishchenko O.V., Zuyev I.V., Avramov A.P. Content of metals in Phragmites australis Trin. ex Steud and Potamogeton pectinatus L. from water bodies of different salinity. J. Sib. Fed. Univ. Biol. 2015;8(3):347­361. DOI:10.17516/1997­1389­2015­8­3­347­361. (in Russian)Ivanova Ye.A., Anishchenko O.V., Zuyev I.V., Avramov A.P. Content of metals in Phragmites australis Trin. ex Steud and Potamogeton pectinatus L. from water bodies of different salinity. J. Sib. Fed. Univ. Biol. 2015;8(3):347­361. DOI:10.17516/1997­1389­2015­8­3­347­361. (in Russian)Kaplan Z. A taxonomic revision of Stuckenia (Potamogetonaceae) in Asia, with notes on the diversity and variation of the genus on a worldwide scale. Folia Geobot. 2008;43(2):159-234. DOI:10.1007/s12224-008-9010-0.Kaplan Z. A taxonomic revision of Stuckenia (Potamogetonaceae) in Asia, with notes on the diversity and variation of the genus on a worldwide scale. Folia Geobot. 2008;43(2):159-234. DOI:10.1007/s12224-008-9010-0.Kaplan Z., Fehrer J., Hellquist C.B. New hybrid combinations revealed by molecular analysis: the unknown side of North American pondweed diversity (Potamogeton). Syst. Bot. 2009;34(4):625-642. DOI:10.1600/036364409790139745.Kaplan Z., Fehrer J., Hellquist C.B. New hybrid combinations revealed by molecular analysis: the unknown side of North American pondweed diversity (Potamogeton). Syst. Bot. 2009;34(4):625-642. DOI:10.1600/036364409790139745.Kaplan Z. Hybridization of Potamogeton species in the Czech Republic: diversity, distribution, temporal trends and habitat preferences. Preslia. 2010;82(3):261-287.Kaplan Z. Hybridization of Potamogeton species in the Czech Republic: diversity, distribution, temporal trends and habitat preferences. Preslia. 2010;82(3):261-287.Kaplan Z., Jarolimova V., Fehrer J. Revision of chromosome numbers of Potamogetonaceae: a new basis for taxonomic and evolutionary implications. Preslia. 2013;85:421-482.Kaplan Z., Jarolimova V., Fehrer J. Revision of chromosome numbers of Potamogetonaceae: a new basis for taxonomic and evolutionary implications. Preslia. 2013;85:421-482.Kaplan Z. Phenotypic plasticity in Potamogeton (Potamogetonaceae). Folia Geobot. 2002;37(2):141-170. DOI:10.1007/BF02804229.Kaplan Z. Phenotypic plasticity in Potamogeton (Potamogetonaceae). Folia Geobot. 2002;37(2):141-170. DOI:10.1007/BF02804229.Kaplan Z., Stepanek J. Genetic variation within and between populations of Potamogeton pusillus agg. J. Plant Syst. Evol. 2003;239(1): 95-112. DOI:10.1007/s00606­002­0252­7.Kaplan Z., Stepanek J. Genetic variation within and between populations of Potamogeton pusillus agg. J. Plant Syst. Evol. 2003;239(1): 95-112. DOI:10.1007/s00606­002­0252­7.Kashina L.I. Роtаmogenaсеае. In: Krasnoborov I.M. (Ed). Flora Sibiri. Lycopodiaceae – Hydrocharitaceae. Novosibirsk: Nauka, 1988;93-105. (in Russian)Kashina L.I. Роtаmogenaсеае. In: Krasnoborov I.M. (Ed). Flora Sibiri. Lycopodiaceae – Hydrocharitaceae. Novosibirsk: Nauka, 1988;93-105. (in Russian)King R.A., Gornall R.J., Preston C.D., Croft J.M. Molecular confirmation of Potamogeton × bottnicus (P. pectinatus × P. vaginatus, Potamogetonaceae) in Britain. Bot. J. Lin. Soc. 2001;135(1):67-70. DOI:10.1111/j.1095­8339.2001.tb02370.x.King R.A., Gornall R.J., Preston C.D., Croft J.M. Molecular confirmation of Potamogeton × bottnicus (P. pectinatus × P. vaginatus, Potamogetonaceae) in Britain. Bot. J. Lin. Soc. 2001;135(1):67-70. DOI:10.1111/j.1095­8339.2001.tb02370.x.Kress W.J., Wurdack K.J., Zimmer E.A., Weigt L.A., Janzen D.H. Use of DNA barcodes to identify flowering plants. PNAS. 2005; 102(23):8369-8374. DOI:10.1073/pnas.0503123102.Kress W.J., Wurdack K.J., Zimmer E.A., Weigt L.A., Janzen D.H. Use of DNA barcodes to identify flowering plants. PNAS. 2005; 102(23):8369-8374. DOI:10.1073/pnas.0503123102.Les D.H. Taxonomic implications of aneuploidy and polyploidy in Potamogeton (Potamogetonaceae). Rhodora. 1983;85(843):301-323.Les D.H. Taxonomic implications of aneuploidy and polyploidy in Potamogeton (Potamogetonaceae). Rhodora. 1983;85(843):301-323.Les D.H., Haynes R.R. Coleogeton (Potamogetonaceae), a new genus of pondweeds. Novon. 1996;6:389-391. DOI:10.2307/3392046.Les D.H., Haynes R.R. Coleogeton (Potamogetonaceae), a new genus of pondweeds. Novon. 1996;6:389-391. DOI:10.2307/3392046.Lindqvist C., De Laet J., Haynes R.R., Aagesen L., Keener B.R., Albert V.A. Molecular phylogenetics of an aquatic plant lineage, Potamogetonaceae. Cladistics. 2006;22(6):568-588. DOI:10.1111/j.1096-0031.2006.00124.x.Lindqvist C., De Laet J., Haynes R.R., Aagesen L., Keener B.R., Albert V.A. Molecular phylogenetics of an aquatic plant lineage, Potamogetonaceae. Cladistics. 2006;22(6):568-588. DOI:10.1111/j.1096-0031.2006.00124.x.Mader E., van Vierssen W., Schwenk K. Clonal diversity in the submerged macrophyte Potamogeton pectinatus L. inferred from nuclear and cytoplasmic variation. Aquat. Bot. 1998;62(3):147-160. DOI:10.1016/S0304­3770(98)00096­5.Mader E., van Vierssen W., Schwenk K. Clonal diversity in the submerged macrophyte Potamogeton pectinatus L. inferred from nuclear and cytoplasmic variation. Aquat. Bot. 1998;62(3):147-160. DOI:10.1016/S0304­3770(98)00096­5.McMullan J.J., Gornall R.J., Preston C. ITS rDNA polymorphism among species and hybrids of Potamogeton subgenus Coleogeton (Potamogetonaceae) in north-western Europe. New J. Bot. 2011; 1(2):111-115. DOI:10.1179/204234811X13194453002788.McMullan J.J., Gornall R.J., Preston C. ITS rDNA polymorphism among species and hybrids of Potamogeton subgenus Coleogeton (Potamogetonaceae) in north-western Europe. New J. Bot. 2011; 1(2):111-115. DOI:10.1179/204234811X13194453002788.Myaemets A.A. Potamogeton. In: Fedorova A.A. (Ed.). Flora Flora of the European Part of the USSR. Vol. 4. Leningrad: Nauka, 1979; 176-192. (in Russian)Myaemets A.A. Potamogeton. In: Fedorova A.A. (Ed.). Flora Flora of the European Part of the USSR. Vol. 4. Leningrad: Nauka, 1979; 176-192. (in Russian)Rogers S.O., Bendich A.J. Extraction of DNA from milligram amounts of fresh herbarium and mummified plant tissues. Plant Mol. Biol. 1985;5(2):69-76. DOI:10.1007/BF00020088.Rogers S.O., Bendich A.J. Extraction of DNA from milligram amounts of fresh herbarium and mummified plant tissues. Plant Mol. Biol. 1985;5(2):69-76. DOI:10.1007/BF00020088.Sang T., Crawford D.J., Stuessy T.F. Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution. PNAS. 1995;92(15):6813-6817. DOI:10.1073/pnas.92.15.6813.Sang T., Crawford D.J., Stuessy T.F. Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution. PNAS. 1995;92(15):6813-6817. DOI:10.1073/pnas.92.15.6813.Shneyer V.S., Rodionov A.V. Plant DNA barcodes. Biol. Bull. Rev. 2019;9(4):295-3900. DOI:10.1134/S207908641904008X.Shneyer V.S., Rodionov A.V. Plant DNA barcodes. Biol. Bull. Rev. 2019;9(4):295-3900. DOI:10.1134/S207908641904008X.Sorsa P. Pollen morphology of Potamogeton and Groenlandia (Potamogetonaceae) and its taxonomic significance. Ann. Bot. Fenn. 1988;25(2):179-199.Sorsa P. Pollen morphology of Potamogeton and Groenlandia (Potamogetonaceae) and its taxonomic significance. Ann. Bot. Fenn. 1988;25(2):179-199.Tamura K., Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 1993;10(3):512-526. DOI:10.1093/oxfordjournals.molbev.a040023.Tamura K., Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 1993;10(3):512-526. DOI:10.1093/oxfordjournals.molbev.a040023.Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 2011;28(10):2731-2739. DOI:10.1093/molbev/msr121.Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 2011;28(10):2731-2739. DOI:10.1093/molbev/msr121.Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22(22):4673-4680. DOI:10.1093/nar/22.22.4673.Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22(22):4673-4680. DOI:10.1093/nar/22.22.4673.Tsvelev N.N. On the species of the subgenus Coleogeton of the genus Potamogeton (Potamogetonaceae) in North-Western Russia. Botanicheskii Zhurnal. 1996;81(7):88-91. (in Russian)Tsvelev N.N. On the species of the subgenus Coleogeton of the genus Potamogeton (Potamogetonaceae) in North-Western Russia. Botanicheskii Zhurnal. 1996;81(7):88-91. (in Russian)Uehara K., Tanaka N., Momohara A., Zhou Z.­K. Genetic diversity of an endangered aquatic plant. Potamogeton lucens subspecies sinicus. Aquat. Bot. 2006;85(4):350-354. DOI:10.1016/j.aquabot.2006.06.008.Uehara K., Tanaka N., Momohara A., Zhou Z.­K. Genetic diversity of an endangered aquatic plant. Potamogeton lucens subspecies sinicus. Aquat. Bot. 2006;85(4):350-354. DOI:10.1016/j.aquabot.2006.06.008.Volkova P.A., Kipriyanova L.M., Maltseva S.Y., Bobrov A.A. In search of speciation: Diversification of Stuckenia pectinata sl (Potamogetonaceae) in southern Siberia (Asian Russia). Aquat. Bot. 2017;143: 25-32. DOI:10.1016/j.aquabot.2017.07.003.Volkova P.A., Kipriyanova L.M., Maltseva S.Y., Bobrov A.A. In search of speciation: Diversification of Stuckenia pectinata sl (Potamogetonaceae) in southern Siberia (Asian Russia). Aquat. Bot. 2017;143: 25-32. DOI:10.1016/j.aquabot.2017.07.003.Wang Q.D., Zhang T., Wang J.B. Phylogenetic relationships and hybrid origin of Potamogeton species (Potamogetonaceae) distributed in China: insights from the nuclear ribosomal internal transcribed spacer sequence (ITS). Plant Syst. Evol. 2007;267(1-4):65-78. DOI:10.1007/s00606­006­0499­5.Wang Q.D., Zhang T., Wang J.B. Phylogenetic relationships and hybrid origin of Potamogeton species (Potamogetonaceae) distributed in China: insights from the nuclear ribosomal internal transcribed spacer sequence (ITS). Plant Syst. Evol. 2007;267(1-4):65-78. DOI:10.1007/s00606­006­0499­5.Whittall J.B., Hellquist C.B., Schneider E.L., Hodges S.A. Cryptic species in an endangered pondweed community (Potamogeton, Potamogetonaceae) revealed by AFLP markers. Am. J. Bot. 2004; 91(12):2022-2029. DOI:10.3732/ajb.91.12.2022.Whittall J.B., Hellquist C.B., Schneider E.L., Hodges S.A. Cryptic species in an endangered pondweed community (Potamogeton, Potamogetonaceae) revealed by AFLP markers. Am. J. Bot. 2004; 91(12):2022-2029. DOI:10.3732/ajb.91.12.2022.Wiegleb G. Notes on pondweeds – outlines for a monographical treatment of the genus Potamogeton L. Fedded Repertorium. 1988; 99(7-8):249-266.Wiegleb G. Notes on pondweeds – outlines for a monographical treatment of the genus Potamogeton L. Fedded Repertorium. 1988; 99(7-8):249-266.Wiegleb G., Kaplan Z. An account of the species of Potamogeton L. (Potamogetonaceae). Folia Geobot. 1998;33(3):241-316. DOI:10.1007/BF03216205.Wiegleb G., Kaplan Z. An account of the species of Potamogeton L. (Potamogetonaceae). Folia Geobot. 1998;33(3):241-316. DOI:10.1007/BF03216205.

The authors declare that there are no conflicts of interest present.