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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJGB-23-22</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-3681</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНЕТИКА ЖИВОТНЫХ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ANIMAL GENETICS</subject></subj-group></article-categories><title-group><article-title>Геногеографическое исследование киргизского горного мериноса с использованием микросателлитных маркеров</article-title><trans-title-group xml:lang="en"><trans-title>A genogeographic study of the Kyrgyz mountain merino via microsatellite markers</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бектуров</surname><given-names>А. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Bekturov</surname><given-names>A. В.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Бишкек</p></bio><bio xml:lang="en"><p>Bishkek</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Исакова</surname><given-names>Ж. Т.</given-names></name><name name-style="western" xml:lang="en"><surname>Isakova</surname><given-names>Zh. T.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Бишкек</p></bio><bio xml:lang="en"><p>Bishkek</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кипень</surname><given-names>В. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Kipen</surname><given-names>V. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Минск</p></bio><bio xml:lang="en"><p>Minsk</p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чортонбаев</surname><given-names>Т. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Chortonbaev</surname><given-names>T. Dzh.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Бишкек</p></bio><bio xml:lang="en"><p>Bishkek</p></bio><email xlink:type="simple">jainagul@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мукеева</surname><given-names>С. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Mukeeva</surname><given-names>S. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Бишкек</p></bio><bio xml:lang="en"><p>Bishkek</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Осмоналиев</surname><given-names>С. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Osmonaliev</surname><given-names>S. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сокулук</p></bio><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Айтбаев</surname><given-names>К. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Aitbaev</surname><given-names>К. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Бишкек</p></bio><bio xml:lang="en"><p>Bishkek</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Кыргызский национальный аграрный университет им. К.И. Скрябина<country>Кыргызстан</country></aff><aff xml:lang="en">Kyrgyz National Agrarian University named after K.I. Skryabin<country>Kyrgyzstan</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Научно-исследовательский институт молекулярной биологии и медицины<country>Кыргызстан</country></aff><aff xml:lang="en">Research Institute of Molecular Biology and Medicine<country>Kyrgyzstan</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru">Институт генетики и цитологии, Национальная академия наук Беларуси<country>Беларусь</country></aff><aff xml:lang="en">Institute of Genetics and Cytology, National Academy of Sciences of Belarus<country>Belarus</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru">Кыргызский научно-исследовательский институт животноводства и пастбищ<country>Кыргызстан</country></aff><aff xml:lang="en">Kyrgyz Research Institute of Animal Husbandry and Pastures Sokuluk District<country>Kyrgyzstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>06</day><month>04</month><year>2023</year></pub-date><volume>27</volume><issue>2</issue><fpage>162</fpage><lpage>168</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Бектуров А.Б., Исакова Ж.Т., Кипень В.Н., Чортонбаев Т.Д., Мукеева С.Б., Осмоналиев С.К., Айтбаев К.А., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Бектуров А.Б., Исакова Ж.Т., Кипень В.Н., Чортонбаев Т.Д., Мукеева С.Б., Осмоналиев С.К., Айтбаев К.А.</copyright-holder><copyright-holder xml:lang="en">Bekturov A.В., Isakova Z.T., Kipen V.N., Chortonbaev T.D., Mukeeva S.B., Osmonaliev S.K., Aitbaev К.A.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/3681">https://vavilov.elpub.ru/jour/article/view/3681</self-uri><abstract><p>Проведено геногеографическое изучение породы овец киргизский горный меринос (КГМ). Проанализированы образцы ДНК 109 овец данной породы, разводимых в трех государственных племенных заводах (ГПЗ) в Республике Кыргызстан: ГПЗ «Оргочор» (Иссык-Кульская область), ГПЗ «Катта-Талдык» (Ошская область) и ГПЗ им. М.Н. Лущихина (Таласская область). В 12 исследованных микросателлитных маркерах (McM042, INRA006, McM527, ETH152, CSRD247, OarFCB20, INRA172, INRA063, MAF065, MAF214, INRA005, INRA023) идентифицировано 126 аллелей. Число аллелей в каждом локусе варьировало от 6 до 16 при среднем значении 10.500 ± 0.957 аллелей на локус. Определено 67 редких аллелей (с частотой встречаемости менее 5.0 %), что составляет 53.2 % от общего количества выявленных аллелей. Наибольшее количество редких аллелей установлено для STR-маркеров CSRD247, INRA023, INRA005, INRA006, MAF214 и OarFCB20. Для каждой группы имеются индивидуальные различия в профиле распределения частот аллелей по всем исследуемым STR-локусам, наиболее значимые из которых следующие: в группах TALAS и OSH для локуса McM042 в мажорном состоянии находится аллель 87 (35.6 и 45.7 % соответственно), в то время как для группы ISSYK-KUL наибольшую распространенность получил аллель 95 (36.2 %); для локуса INRA172 во всех группах мажорным аллелем был 154, однако в сравнении с группой TALAS его распространенность была меньше в 1.25 (ISSYK-KUL) и 1.66 (OSH) раза – 55.2 и 41.4 % соответственно, а аллели 156 и 158 встречались только в группе ISSYK-KUL; для локуса ETH152 частота встречаемости аллеля 186 в группе TALAS составила 51.1 %, для групп ISSYK-KUL и OSH значительную распространенность приобретает аллель 190 – 34.5 и 34.3 % соответственно. При оценке генетической подразделенности исследуемых выборок КГМ (при К от 3 до 10) показана однородность структуры – вклад каждого субкластера равноценный. При анализе AMOVA обнаружено, что выборки расположены равноудаленно. Таким образом, генетическое разнообразие овец породы КГМ среди трех государственных племенных заводов Кыргызской Республики достаточно высокое и сопоставимое между собой.</p></abstract><trans-abstract xml:lang="en"><p>The aim was to ascertain the genetic and geographical structure of the Kyrgyz mountain merino (KMM). We analyzed DNA samples of 109 Kyrgyz mountain merino specimens, bred in three state breeding factories (STB), including“Orgochor” in the Issykul Province,“Katta-Taldyk” in the Osh Province and STb named after Luschikhin in the Talas Province. We identified 126 alleles in 12 microsatellite markers (McM042, INRA006, McM527, ETH152, CSRD247, OarFCB20, INRA172, INRA063, MAF065, MAF214, INRA005, INRA023). There were 6 to 16 alleles in each locus (mean 10.500 ± 0.957 alleles per locus). We identified 67 rare alleles (prevalence less than 5.0 %), which made up 53.2 % of all alleles found. The greatest number of rare alleles was found in STR-markers of CSRD247, INRA023, INRA005, INRA006, MAF214 and OarFCB20. For each group, there were individual differences in the distribution of allele frequencies across all the STR loci studied. The most significant of them were as follows: with regard to the McM042 locus, allele 87 was major in the TALAS and OSH groups (35.6 and 45.7 %, respectively), whereas allele 95 was major in the ISSYK-KUL group (36.2 %); allele 154 was major in all groups with regard to the INRA172 locus, but it was 1.25 times less prevalent in the ISSYK-KUL and 1.66 times less prevalent in the OSH groups compared to TALAS (55.2 and 41.4 %, respectively), whereas alleles 156 and 158 were found only in the ISSYK-KUL group. Considering the ETH152 locus, 186 allele prevalence in the TALAS group was 51.1 %, but allele 190 was also markedly prevalent in the ISSYK-KUL and OSH groups, 34.5 and 34.3 %, respectively. The genetic division of the studied groups of KMM (with K from 3 to 10) was homogeneous – the contribution of each subcluster was equivalent. The AMOVA analysis revealed that the groups are located equidistantly. To conclude, the genetic diversity of the Kyrgyz mountain merino in three state breeding factories of the Kyrgyz Republic was high and comparable with each other.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>киргизский горный меринос</kwd><kwd>генотипирование</kwd><kwd>STR-маркеры</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Kyrgyz mountain merino</kwd><kwd>genotyping</kwd><kwd>STR markers</kwd></kwd-group><funding-group xml:lang="en"><funding-statement>The study was carried out with the financial support of the Ministry of Education and Science of the Kyrgyz Republic within the framework of the scientific project “The use of molecular genetic research methods and the creation of information resources for the intensification of sheep breeding in the Kyrgyz Republic”</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Ahmed Z., Babar M.E., Hussain T., Awan F.I. 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