vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-25vavilov-3730Research ArticleГЕНОМИКА И ТРАНСКРИПТОМИКАPLANT GENETICS AND BREEDINGSWEET транспортеры Medicago lupulina в арбускулярно-микоризной системе в условиях среднего уровня доступного фосфораSWEET transporters of Medicago lupulina in the arbuscular-mycorrhizal system in the presence of medium level of available phosphorushttps://orcid.org/0000-0002-8715-6723КрюковА. А.KryukovA. A.

Пушкин, Санкт-Петербург

Pushkin, St. Petersburg

rainniar@rambler.ruhttps://orcid.org/0009-0002-5624-1244ГорбуноваА. О.GorbunovaA. O.

Пушкин, Санкт-Петербург

Pushkin, St. Petersburg

https://orcid.org/0000-0001-5120-7229КудряшоваТ. Р.KudriashovaT. R.

Пушкин, Санкт-Петербург

Pushkin, St. Petersburg

https://orcid.org/0000-0003-3657-2986ИванченкоО. Б.IvanchenkoO. B.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0002-2231-6466ШишоваМ. ф.ShishovaM. F.

Биологический факультет

Санкт-Петербург

Biological Faculty

St. Petersburg

https://orcid.org/0000-0002-1311-1258ЮрковА. П.YurkovA. P.

Пушкин, Санкт-Петербург

Pushkin, St. Petersburg

Всероссийский научно-исследовательский институт сельскохозяйственной микробиологииРоссияAll-Russia Research Institute for Agricultural MicrobiologyRussian FederationВсероссийский научно-исследовательский институт сельскохозяйственной микробиологии; Санкт-Петербургский политехнический университет Петра ВеликогоРоссияAll-Russia Research Institute for Agricultural Microbiology; Peter the Great St. Petersburg Polytechnic UniversityRussian FederationСанкт-Петербургский политехнический университет Петра ВеликогоРоссияPeter the Great St. Petersburg Polytechnic UniversityRussian FederationСанкт-Петербургский государственный университетРоссияSaint Petersburg State UniversityRussian FederationВсероссийский научно-исследовательский институт сельскохозяйственной микробиологииРоссия1 All-Russia Research Institute for Agricultural MicrobiologyRussian Federation202302062023273189196Copyright © Крюков А.А., Горбунова А.О., Кудряшова Т.Р., Иванченко О.Б., Шишова М.ф., Юрков А.П., 20232023Крюков А.А., Горбунова А.О., Кудряшова Т.Р., Иванченко О.Б., Шишова М.ф., Юрков А.П.Kryukov A.A., Gorbunova A.O., Kudriashova T.R., Ivanchenko O.B., Shishova M.F., Yurkov A.P.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3730

Грибы арбускулярной микоризы (АМ) помогают растениям усваивать из почвы минеральные вещества, особенно фосфор, в то время как получают от растений продукты фотосинтеза – сахара. Выявление генов, контролирующих симбиотическую эффективность АМ, может иметь практическое применение при создании высокопродуктивных растительно-микробных систем. Поэтому целью работы была оценка уровней экспрессии генов семейства транспортеров сахаров SWEET – единственного семейства, где могут быть обнаружены специфические для АМ-симбиоза транспортеры сахаров. Нами разработана уникальная высокочувствительная к уровню фосфора и микоризации модельная система «растение-хозяин–АМ-гриб», включающая высокоотзывчивую на инокуляцию АМ-грибами экологически облигатно микотрофную линию MlS-1 люцерны хмелевидной (Medicago lupulina) и штамм АМ-гриба RCAM00320 Rhizophagus irregularis, обладающий высокой эффективностью на множестве видов растений. С использованием этой модельной системы в корнях растения оценены изменения в уровне экспрессии 11 генов, кодирующих транспортеры семейства SWEET, при развитии либо при отсутствии симбиоза M. lupulina с R. irregularis в различные фазы развития растения-хозяина в условиях среднего уровня доступного для питания растений фосфора в субстрате. У микоризованных растений обнаружены более высокие уровни экспрессии в большинстве фаз развития растения-хозяина для MlSWEET1b, MlSWEET3c, MlSWEET12, MlSWEET13 по сравнению с контролем без АМ. Повышенная относительно контроля экспрессия при микоризации наблюдалась также и для MlSWEET11 в фазу развития 2-го и 3-го листьев, для MlSWEET15с – в фазу стеблевания, для MlSWEET1а – в фазу 2-го листа, стеблевания и бокового ветвления. Ген MlSWEET1b с уверенностью можно считать хорошим маркером со специфической экспрессией для эффективного развития АМ-симбиоза M. lupulina с R. irregularis в условиях среднего уровня доступного для растений фосфора в субстрате.

Arbuscular mycorrhiza (AM) fungi receive photosynthetic products and sugars from plants in exchange for contributing to the uptake of minerals, especially phosphorus, from the soil. The identification of genes controlling AM symbiotic efficiency may have practical application in the creation of highly productive plant-microbe systems. The aim of our work was to evaluate the expression levels of SWEET sugar transporter genes, the only family in which sugar transporters specific to AM symbiosis can be detected. We have selected a unique “host plant–AM fungus” model system with high response to mycorrhization under medium phosphorus level. This includes a plant line which is highly responsive to inoculation by AM fungi, an ecologically obligate mycotrophic line MlS-1 from black medick (Medicago lupulina) and the AM fungus Rhizophagus irregularis strain RCAM00320, which has a high efficiency in a number of plant species. Using the selected model system, differences in the expression levels of 11 genes encoding SWEET transporters in the roots of the host plant were evaluated during the development of or in the absence of symbiosis of M. lupulina with R. irregularis at various stages of the host plant development in the presence of medium level of phosphorus available for plant nutrition in the substrate. At most stages of host plant development, mycorrhizal plants had higher expression levels of MlSWEET1b, MlSWEET3c, MlSWEET12 and MlSWEET13 compared to AM-less controls. Also, increased expression relative to control during mycorrhization was observed for MlSWEET11 at 2nd and 3rd leaf development stages, for MlSWEET15c at stemming (stooling) stage, for MlSWEET1a at 2nd leaf development, stemming and lateral branching stages. The MlSWEET1b gene can be confidently considered a good marker with specific expression for effective development of AM symbiosis between M. lupulina and R. irregularis in the presence of medium level of phosphorus available to plants in the substrate.

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The authors declare that there are no conflicts of interest present.