vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-31vavilov-3736Research ArticleГЕНЕТИКА НАСЕКОМЫХINSECT GENETICSВлияние LIM-киназы 1 на память и брачную песню cамцов дрозофилы зависит от типа нейроновLIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal typehttps://orcid.org/0000-0003-2673-4283ЖуравлевА. В.ZhuravlevA. V.

Санкт-Петербург

St. Petersburg

beneor@mail.ruhttps://orcid.org/0000-0002-6005-3433ЗаломаеваЕ. С.ZalomaevaE. S.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0002-0055-3778ЕгозоваЕ. С.EgozovaE. S.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0002-8498-9017ЕмелинА. Д.EmelinA. D.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0003-0821-9974СокуроваВ. В.SokurovaV. V.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0003-1897-8392НикитинаЕ. И.NikitinaE. A.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0002-6925-4370Савватеева-ПоповаЕ. В.Savvateeva-PopovaE. V.

Санкт-Петербург

St. Petersburg

Институт физиологии им. И.П. Павлова Российской академии наукРоссияPavlov Institute of Physiology of the Russian Academy of SciencesRussian FederationИнститут физиологии им. И.П. Павлова Российской академии наук; Российский государственный педагогический университет им. А.И. ГерценаРоссияPavlov Institute of Physiology of the Russian Academy of Sciences; Herzen State Pedagogical University of RussiaRussian FederationРоссийский государственный педагогический университет им. А.И. ГерценаРоссияHerzen State Pedagogical University of RussiaRussian Federation202302062023273250263Copyright © Журавлев А.В., Заломаева Е.С., Егозова Е.С., Емелин А.Д., Сокурова В.В., Никитина Е.И., Савватеева-Попова Е.В., 20232023Журавлев А.В., Заломаева Е.С., Егозова Е.С., Емелин А.Д., Сокурова В.В., Никитина Е.И., Савватеева-Попова Е.В.Zhuravlev A.V., Zalomaeva E.S., Egozova E.S., Emelin A.D., Sokurova V.V., Nikitina E.A., Savvateeva-Popova E.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3736

Сигнальный каскад ремоделирования актина, в состав которого входят LIM-киназа 1 (LIMK1) и ее субстрат кофилин, участвует в регуляции различных процессов в нейронах позвоночных и беспозвоночных животных. Drosophila melanogaster широко используется как модельный объект для изучения механизмов формирования, сохранения и воспроизведения памяти, а также забывания. Ранее активное забывание у дрозофилы исследовали с помощью классического павловского ольфакторного обучения. Было показано, что в разных формах забывания участвуют специфические дофаминергические нейроны и компоненты актинового каскада. В данной работе мы оценивали роль LIMK1 в процессах памяти и забывания у дрозофилы в парадигме условно-рефлекторного подавления ухаживания. В мозге дрозофилы уровень LIMK1 и фосфокофилина избирательно снижен в отдельных структурах нейропиля, включая лопасти грибовидных тел и центральный комплекс. В то же время LIMK1 присутствует в телах нервных клеток, таких как кластеры дофаминергических нейронов, регулирующие формирование памяти при условно-рефлекторном подавлении ухаживания. С использованием системы бинарного скрещивания GAL4 × UAS мы инициировали РНК-интерференцию limk1 в различных типах нервных клеток. У гибридных линий с интерференцией limk1 в лопастях грибовидных тел и глии наблюдалось усиление 3-часовой краткосрочной памяти, без видимого влияния на долгосрочную память. Интерференция limk1 в холинергических нейронах приводила к снижению краткосрочной памяти, в дофаминергических и серотонинергических нейронах ее результатом было также существенное нарушение способности мух к обучению. Напротив, интерференция limk1 в нейронах fruitless усиливала 15–60-минутную краткосрочную память, что указывает на возможную роль LIMK1 в процессах активного забывания. У самцов с интерференцией limk1 в  холинергических и fruitless нейронах также были отмечены разнонаправленные изменения параметров брачной песни. Таким образом, эффекты LIMK1 на память и брачную песню самцов дрозофилы определяются типом нервных клеток или структурой мозга.

The signal pathway of actin remodeling, including LIM-kinase 1 (LIMK1) and its substrate cofilin, regulates multiple processes in neurons of vertebrates and invertebrates. Drosophila melanogaster is widely used as a model object for studying mechanisms of memory formation, storage, retrieval and forgetting. Previously, active forgetting in Drosophila was investigated in the standard Pavlovian olfactory conditioning paradigm. The role of specific dopaminergic neurons (DAN) and components of the actin remodeling pathway in different forms of forgetting was shown. In our research, we investigated the role of LIMK1 in Drosophila memory and forgetting in the conditioned courtship suppression paradigm (CCSP). In the Drosophila brain, LIMK1 and p-cofilin levels appeared to be low in specific neuropil structures, including the mushroom body (MB) lobes and the central complex. At the same time, LIMK1 was observed in cell bodies, such as DAN clusters regulating memory formation in CCSP. We applied GAL4 × UAS binary system to induce limk1 RNA interference in different types of neurons. The hybrid strain with limk1 interference in MB lobes and glia showed an increase in 3-h short-term memory (STM), without significant effects on long-term memory. limk1 interference in cholinergic neurons (CHN) impaired STM, while its interference in DAN and serotoninergic neurons (SRN) also dramatically impaired the flies’ learning ability. By contrast, limk1 interference in fruitless neurons (FRN) resulted in increased 15–60 min STM, indicating a possible LIMK1 role in active forgetting. Males with limk1 interference in CHN and FRN also showed the opposite trends of courtship song parameters changes. Thus, LIMK1 effects on the Drosophila male memory and courtship song appeared to depend on the neuronal type or brain structure.

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The authors declare that there are no conflicts of interest present.