vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-37vavilov-3768Research ArticleГЕНОМИКА И ТРАНСКРИПТОМИКАPLANT GENETICS AND BREEDINGМорфологические и филогенетические особенности крымской популяции Juniperus deltoids R.P. AdamsMorphological and phylogenetic features of the Crimean population of Juniperus deltoids R.P. Adamshttps://orcid.org/0000-0003-3614-9387ЛантушенкоА. О.LantushenkoA. O.

Севастополь

Sevastopol 

https://orcid.org/0000-0001-6482-7312КореньковаО. О.KorenkovaO. O.

Ялта, Республика Крым

Yalta, Republik of the Crimea

o.o.korenkova@mail.ruСыровецА. А.SyrovetsA. A.

Севастополь

Sevastopol 

МегерЯ. В.MegerYa. V.

Севастополь

Sevastopol 

https://orcid.org/0000-0003-1847-4303КореньковП. А.KorenkovP. A.

Москва

Moscow

https://orcid.org/0000-0002-6565-7930ШевчукО. М.ShevchukO. M.

Ялта, Республика Крым

Yalta, Republik of the Crimea

Севастопольский государственный университетРоссияSevastopol State UniversityRussian FederationОрдена Трудового Красного Знамени Никитский ботанический сад – Национальный научный центр Российской академии наукРоссияThe Order of the Red Banner of Labour Nikitsky Botanical Gardens – National Scientific Center of the Russian Academy of SciencesRussian FederationНациональный исследовательский Московский государственный строительный университетРоссияMoscow State University of Civil Engineering (National Research University)Russian Federation202313072023274306315Copyright © Лантушенко А.О., Коренькова О.О., Сыровец А.А., Мегер Я.В., Кореньков П.А., Шевчук О.М., 20232023Лантушенко А.О., Коренькова О.О., Сыровец А.А., Мегер Я.В., Кореньков П.А., Шевчук О.М.Lantushenko A.O., Korenkova O.O., Syrovets A.A., Meger Y.V., Korenkov P.A., Shevchuk O.M.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3768

Juniperus deltoides – это реликтовый вид третичного периода. Является типичным представителем средиземноморской группы секции Juniperus. Включен в Красные книги Республики Крым и города Севастополя. До недавнего времени считалось, что на территории Крыма произрастает популяция J. oxycedrus. Сейчас J. deltoides описывают как криптический вид, морфологически сложно отличимый от J. oxycedrus. В результате возникла необходимость проведения ряда детальных исследований по определению морфологических и филогенетических особенностей криптической популяции Крыма с целью установления ее принадлежности к одному из видов криптической пары. Исследования проходили в два этапа: на первом этапе определяли морфологические особенности вегетативных и генеративных органов и их отличие от J. oxycedrus, на втором – выполняли генетические исследования. Длина хвои крымской популяции составляет 12.94 ± 0.19 мм, что соответствует восточноитальянской популяции J. deltoides. При этом ширина хвои равна 1.39 ± 0.02 мм, что свойственно португальской популяции J. oxycedrus. Размеры шишкоягод составляют: d1 (условно высота) – 7.54 ± 0.14 мм, d2 (условно ширина) – 9.11 ± 0.09 мм, что больше соответствует J. deltoides. Форма шишкоягод варьирует весьма сильно. Встречаются особи с шишкоягодами, кроющие чешуи которых визуально не отличимы, их верхушки полностью срастаются. Подобное явление характерно для западносредиземноморских популяций J. oxycedrus. Морфологический анализ вегетативных и генеративных органов J. deltoides показал, что при сочетании двух этих признаков достоверно различить особи J. deltoides и J. oxycedrus не представляется возможным. Для генетического анализа использовали ядерные (внутренний транскрибируемый спейсер ITS) и хлоропластные (petN-psbM, trnS-trnG) некодирующие участки генома. Исследования показали, что ядерные участки генов обладают большей вариабельностью, чем хлоропластные. Последовательности, полученные в данной работе, образовали кладу с образцами J. deltoides 9430 и 9431 (BAYLU), произрастающими в Турции, что позволяет отнести изученные образцы к виду J. deltoides.

Juniperus deltoides is a relict species from the Tertiary Period. It is a typical representative of the Mediterranean group of the section Juniperus. It is included in the Red Books of the Republic of Crimea and the city of Sevastopol. Until recently, it was believed that a population of J. oxycedrus grew in Crimea. Currently, J. deltoides is described as a cryptic species, morphologically difficult to distinguish from J. oxycedrus. As a result, it became necessary to conduct a series of detailed studies to determine the morphological and phylogenetic features of the Crimean cryptic population in order to identify it as being one of the species of the cryptic pair. The studies were carried out in two stages: at the first stage, the morphological features of the vegetative and generative organs and their difference from J. oxycedrus were determined; the second stage included genetic research. The length of the needles of the Crimean population is 12.94 ± 0.19 mm, which corresponds to the Eastern Italian population of J. deltoides. At the same time, the width of the needles is 1.39 ± 0.02 mm, which is typical of the Portuguese population of J. oxycedrus. The dimensions of the cones are d1 (conditional height) = 7.54 ± 0.14 mm, and d2 (conditional width) = 9.11 ± 0.09 mm, which is more in line with J. deltoides. The shapes of the cones are very diverse. Some individuals have cones, the covering scales of which are visually indistinguishable, and their tops are completely fused. A similar phenomenon is characteristic of the Western Mediterranean populations of J. oxycedrus. Morphological analysis of the vegetative and generative organs of J. deltoides showed that when these two traits are combined, it is not possible to reliably distinguish between J. deltoides and J. oxycedrus individuals. Nuclear (ITS internal transcribed spacer) and chloroplast (petN-psbM, trnS-trnG) non-coding regions of the genome were used for genetic analysis. Studies have shown that the nuclear regions of genes have greater variability than chloroplast regions. The sequences obtained in this work formed a clade with J. deltoides samples 9430 and 9431 (BAYLU) growing in Turkey, which makes it possible to assign the samples studied to J. deltoides.

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The authors declare that there are no conflicts of interest present.