vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-58vavilov-3859Research ArticleГЕНЕТИКА ЖИВОТНЫХANIMAL GENETICSСамки мышей CD-1 распознают репродуктивно успешных самцов по летучим органическим соединениям их мочиCD-1 mice females recognize male reproductive success via volatile organic compounds in urinehttps://orcid.org/0000-0001-5379-9977ХоцкинаА. С.KhotskinaA. S.

Новосибирск

Novosibirsk

dotcenko@bionet.nsc.ruhttps://orcid.org/0000-0002-9412-3874ЗавьяловЕ. Л.ZavjalovE. L.

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Novosibirsk

ШнайдерЕ. П.ShnayderE. P.

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Novosibirsk

https://orcid.org/0000-0002-8118-1362ГерлинскаяЛ. A.GerlinskayaL. A.

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Novosibirsk

МасленниковаС. О.MaslennikovaS. O.

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Novosibirsk

https://orcid.org/0000-0002-0623-0363ПетровскийД. В.PetrovskiiD. V.

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Novosibirsk

БалдинМ. Н.BaldinM. N.

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Novosibirsk

МакасьА. Л.MakasA. L.

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Novosibirsk

ГрузновВ. М.GruznovV. M.

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Novosibirsk

ТрошковМ. Л.TroshkovM. L.

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Novosibirsk

https://orcid.org/0000-0002-5388-2946МошкинМ. П.MoshkinM. P.

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Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationИнститут нефтегазовой геологии и геофизики им. А.А. Трофимука Сибирского отделения Российской академии наукРоссияTrofimuk Institute of Petroleum Geology and Geophysics of the Siberian Branch of the Russian Academy of SciencesRussian Federation202308092023275480487Copyright © Хоцкина А.С., Завьялов Е.Л., Шнайдер Е.П., Герлинская Л.A., Масленникова С.О., Петровский Д.В., Балдин М.Н., Макась А.Л., Грузнов В.М., Трошков М.Л., Мошкин М.П., 20232023Хоцкина А.С., Завьялов Е.Л., Шнайдер Е.П., Герлинская Л.A., Масленникова С.О., Петровский Д.В., Балдин М.Н., Макась А.Л., Грузнов В.М., Трошков М.Л., Мошкин М.П.Khotskina A.S., Zavjalov E.L., Shnayder E.P., Gerlinskaya L.A., Maslennikova S.O., Petrovskii D.V., Baldin M.N., Makas A.L., Gruznov V.M., Troshkov M.L., Moshkin M.P.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3859

Половой отбор рассматривается в качестве одного из ведущих факторов эволюционного развития. В условиях постоянной конкуренции сформировались специализированные способы привлечения особей противоположного пола, а также критерии оценки качества полового партнера. Самцы, способные оставить наибольшее количество потомков, должны быть более привлекательными половыми партнерами, что требует от них каким-либо образом демонстрировать самкам свои преимущества. При этом ключевое условие для реализации такого отбора – это способность сигнала достоверно отражать информацию о физиологическом состоянии организма особи. У мышей одним из ведущих каналов передачи информации являются хемосигналы. Целью нашего исследования было выяснить, могут ли самцы мышей демонстрировать самкам свою репродуктивную эффективность через летучие органические соединения мочи. В ходе эксперимента самцу подсаживали двух половозрелых самок на шесть дней для размножения. Репродуктивный успех самца оценивали по наличию или отсутствию потомства. В то же время половозрелые самки, ранее не участвовавшие в размножении, оценивали мочу этих самцов. Оказалось, что более привлекательной была моча самцов, способных оставить потомство. Это выражалось в более коротком латентном времени обнюхивания самкой мочи самца в ольфакторном тесте. При помощи газовой хроматографии в моче самцов были обнаружены летучие органические соединения, которые коррелировали с поведением самок. Масс-спектрометрический анализ образцов мочи показал, что эти соединения являются производными феромона мышей 6-гидрокси-6-метил-3-гептанона. Амплитуда пиков, соответствующих данному феромону, коррелировала с уровнем тестостерона в крови и массой препуциальных желез и была выше у самцов с фертильными покрытиями. Важно отметить, что ключевые морфофизиологические показатели, такие как масса тела, масса семенников, препуциальных желез, семенных пузырьков, а также уровень тестостерона в плазме крови самцов, не отражали их способность оставить потомство. Таким образом, содержание феромона 6-гидрокси-6-метил-3-гептанона в моче самцов CD-1 может служить хорошим критерием их репродуктивной эффективности.

Sexual selection is considered as one of the leading factors of evolutionary development. In the conditions of incessant competition, specialized methods of attracting individuals of the opposite sex as well as criteria for assessing the quality of a sexual partner have been formed. In order for animals to rely on signaling from sexual partners, the signal must reflect the morpho-physiological status of animals. A high reproductive efficiency of male mice is a good advantage for mate selection and thus must be somehow demonstrated to potential mates. The aim of our study was to find out if male mice could demonstrate their reproductive efficiency through urine volatile organic compounds. The experiment implies cohabiting one male with two mature females for 6 days. The reproductive success of the male was assessed by the presence or absence of pregnant females. At the same time, naive females, who did not participate in reproduction, assessed the urine of the successful males as more attractive, which was expressed in shorter Latency time of sniffs in the Olfactory test. Using a rapid headspace GC/MS analysis, we have found volatile organic compounds (VOCs) in male urine that correlated with female behavior. It turned out that these substances are derivatives of mouse pheromone 6-hydroxy-6-methyl-3-heptanone. The amplitude of peaks corresponding to this pheromone correlated with the testosterone level in blood and the weight of preputial glands. The amplitude of peaks increased in males after mating with whom the females turned out to be pregnant. It is important to note that body weight, weight of testes, weight of seminal vesicles, weight of preputial glands, and plasma testosterone level alone are not reliable indicators of male reproductive success. Thus, the content of the pheromone 6-hydroxy-6-methyl-3-heptanone in the urine of males can serve as a good predictor of the quality of the male as a sexual partner for female CD-1 mice.

хемосигналыдигидрофуранГХ/МС6-гидрокси-6-метил-3-гептанонполовое предпочтениеольфакторное предпочтениерепродуктивный успехchemical signalsdihydrofuranGC/MS6-hydroxy-6-methyl-3-heptanonemating preferenceolfactory preferencereproductive successThe study of females behavior was supported by the budget project (FWNR-2022-0004). The GC/MS study was supported by the Russian Science Foundation grant No. 20-14-00055. The studies are implemented using the equipment of the Center for Genetic Resources of Laboratory Animals at ICG SB RAS, supported by the Ministry of Science and Higher Education of Russian Federation (Unique identifier of the project RFMEFI62119X0023). We thank Professor Milos Novotny for the data of mass-spectra of synthetic cyclic enol ethers obtained in his laboratory in Indiana University.ReferencesAndersson M. Condition-dependent indicators in sexual selection: development of theory and tests. In: Lucas J.R., Simmons L.W. (Eds.) 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The authors declare that there are no conflicts of interest present.