vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-59vavilov-3860Research ArticleГЕНЕТИКА ЖИВОТНЫХANIMAL GENETICSДифференциация Bos grunniens и Bos taurus на основании полиморфизма STR-локусовDifferentiation of Bos grunniens and Bos taurus based on STR locus polymorphismhttps://orcid.org/0000-0001-6146-6750ЧекировК. Б.ChekirovK. B.

Бишкек

Bishkek

https://orcid.org/0000-0002-3681-6939ИсаковаЖ. Т.IsakovaZh. T.

Бишкек

Bishkek

jainagul@mail.ruhttps://orcid.org/0000-0002-7822-0746КипеньВ. Н.KipenV. N.

Минск

Minsk

https://orcid.org/0000-0002-8364-8982ИрсалиевМ. И.IrsalievM. I.

Бишкек

Bishkek

https://orcid.org/0000-0001-9584-4860МукееваС. Б.MukeevaS. B.

Бишкек

Bishkek

https://orcid.org/0000-0003-4973-039XАйтбаевК. А.AitbaevK. A.

Бишкек

Bishkek

https://orcid.org/0000-0002-5016-2492ШаршеналиеваГ. А.SharshenalievaG. A.

Бишкек

Bishkek

https://orcid.org/0000-0003-4461-6993БейшеналиеваС. Б.BeyshenalievaS. B.

Бишкек

Bishkek

https://orcid.org/0000-0001-9392-2773КыдыралиеваБ. У.KydyralievaB. U.

Бишкек

Bishkek

Кыргызско-Турецкий университет «Манас»КыргызстанKyrgyz-Turkish Manas UniversityKyrgyzstanНаучно-исследовательский институт молекулярной биологии и медициныКыргызстанResearch Institute of Molecular Biology and MedicineKyrgyzstanИнститут генетики и цитологии Национальной академии наук БеларусиБеларусьInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusBelarusКыргызский государственный университет им. И. АрабаеваКыргызстанKyrgyz State University named after I. ArabaevKyrgyzstan202308092023275488494Copyright © Чекиров К.Б., Исакова Ж.Т., Кипень В.Н., Ирсалиев М.И., Мукеева С.Б., Айтбаев К.А., Шаршеналиева Г.А., Бейшеналиева С.Б., Кыдыралиева Б.У., 20232023Чекиров К.Б., Исакова Ж.Т., Кипень В.Н., Ирсалиев М.И., Мукеева С.Б., Айтбаев К.А., Шаршеналиева Г.А., Бейшеналиева С.Б., Кыдыралиева Б.У.Chekirov K.B., Isakova Z.T., Kipen V.N., Irsaliev M.I., Mukeeva S.B., Aitbaev K.A., Sharshenalieva G.A., Beyshenalieva S.B., Kydyralieva B.U.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3860

Дифференциация близкородственных биологических видов с использованием методов молекулярно-генетического анализа имеет важное значение для селекционных процессов при разведении сельскохозяйственных животных, создании гибридных линий, поддержании генетической чистоты пород, линий, отводок. Подход к дифференциации Bos grunniens и Bos taurus на основании полиморфизма STR-локусов будет способствовать поддержанию генетической обособленности данных видов и, как следствие, выявлению гибридных особей. Целью исследования была оценка дифференцирующего потенциала 15 микросателлитных локусов для различения особей домашнего яка (B. grunniens), разводимых в высокогорном регионе Калмак-Ашуу (Кочкорский район, Нарынская область, Кыргызская Республика), и крупного рогатого скота (B. taurus) трех пород (абердин-ангусская, голштинская и алатауская) с использованием молекулярно-генетического анализа. Образцы были генотипированы по 15 микросателлитным локусам (ETH3, INRA023, TGLA227, TGLA126, TGLA122, SPS115, ETH225, TGLA53, BM2113, BM1824, ETH10, BM1818, CSSM66, ILSTS006 и CSRM60). Двенадцать из рассматриваемых STR-локусов составляли стандартную панель маркеров, рекомендованную ISAG. Статистический анализ данных проводили с использованием программ GenAlEx v.6.503, Structure v.2.3.4, PAST v.4.03 и POPHELPER v1.0.10. Анализ субпопуляционной структуры исследуемых выборок в программе Structure v.2.3.4 по данным генотипирования 15 STR-локусов показал, что точность отнесения образца к B. taurus составила 99.6 ± 0.4 %, к B. grunniens – 99.2 ± 2.6 %. Наибольшим потенциалом для дифференциации B. grunniens и B. taurus обладали те локусы, для которых рассчитанные значения показателя FST оказались максимальными – BM1818 (0.056), BM1824 (0.041), BM2113 (0.030), CSSM66 (0.034) и ILSTS006 (0.063). Точность классификации B. grunniens с использованием только этих пяти микросателлитных локусов составила 98.8 ± 3.4 %, B. taurus – 99.1 ± 1.2 %. Предложенный нами подход, основанный на молекулярно-генетическом анализе пяти STR-локусов, может быть использован в качестве экспресс-теста в селекционных и воспроизводительных программах Кыргызстана для B. grunniens.

Differentiation of closely related biological species using molecular genetic analysis is important for breeding farm animals, creating hybrid lines, maintaining the genetic purity of breeds, lines and layering. Bos grunniens and Bos taurus differentiation based on STR locus polymorphism will help maintain the genetic isolation of these species and identify hybrid individuals. The aim of this study is to assess the differentiating potential of 15 microsatellite loci to distinguish between domestic yak (B. grunniens) bred in the Kalmak-Ashuu highland region (Kochkor district, Naryn region, Kyrgyz Republic) and cattle (B. taurus) of three breeds (Aberdeen-Angus, Holstein and Alatau) using molecular genetic analysis. The samples were genotyped at 15 microsatellite loci (ETH3, INRA023, TGLA227, TGLA126, TGLA122, SPS115, ETH225, TGLA53, BM2113, BM1824, ETH10, BM1818, CSSM66, ILSTS006 and CSRM60). Twelve of the loci were from the standard markers panel recommended by ISAG. Statistical analysis was performed using GenAlEx v.6.503, Structure v.2.3.4, PAST v.4.03, and POPHELPER v1.0.10. The analysis of the samples’ subpopulation structure using the Structure v.2.3.4 and 15 STR locus genotyping showed that the accuracy of assigning a sample to B. taurus was 99.6 ± 0.4 %, whereas the accuracy of assigning a sample to B. grunniens was 99.2 ± 2.6 %. Of the 15 STRs, the greatest potential to differentiate B. grunniens and B. taurus was found in those with the maximal calculated FST values, including BM1818 (0.056), BM1824 (0.041), BM2113 (0.030), CSSM66 (0.034) and ILSTS006 (0.063). The classification accuracy of B. grunniens using only these five microsatellite loci was 98.8 ± 3.4 %, similar for B. taurus, 99.1 ± 1.2 %. The proposed approach, based on the molecular genetic analysis of 5 STR loci, can be used as an express test in Kyrgyzstan breeding and reproduction programs for B. grunniens.

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The authors declare that there are no conflicts of interest present.