vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-68vavilov-3927Research ArticleИММУНИТЕТ РАСТЕНИЙPLANT IMMUNITYПолногеномный анализ ассоциации устойчивости к пепельной гнили сои, выращенной в КазахстанеGenome-wide association study for charcoal rot resistance in soybean harvested in Kazakhstanhttps://orcid.org/0000-0003-4310-5753ЗатыбековА.ZatybekovA.

Алматы

Almaty

https://orcid.org/0000-0002-9748-507XАбугалиеваС.AbugalievaS.

Алматы

Almaty

https://orcid.org/0000-0002-2223-0718ДидоренкоС.DidorenkoS.

пос. Алмалыбак, Алматинская область

Almalybak, Almaty Region

https://orcid.org/0000-0002-9921-6076РсалиевА.RsaliyevA.

пос. Гвардейский (Отар), Жамбылская область

Gvardeiskiy (Otar), Zhambyl Region

https://orcid.org/0000-0001-9535-2997МауленбайА.MaulenbayA.

пос. Гвардейский (Отар), Жамбылская область

Gvardeiskiy (Otar), Zhambyl Region

https://orcid.org/0000-0003-0564-7586ФангЧ.FangC.

Гуанчжоу

Guangzhou

https://orcid.org/0000-0001-8590-1745ТуруспековЕ.TuruspekovY.

Алматы

Almaty

yerlant@yahoo.comИнститут биологии и биотехнологии растенийКазахстанInstitute of Plant Biology and BiotechnologyKazakhstanКазахский научно-исследовательский институт земледелия и растениеводстваКазахстанKazakh Research Institute of Agriculture and Plant GrowingKazakhstanНаучно-исследовательский институт проблем биологической безопасностиКазахстанResearch Institute for Biological Safety ProblemsKazakhstanШкола наук о жизни, Университет ГуанчжоуКитайSchool of Life Sciences, Guangzhou UniversityChina202301112023276565571Copyright © Затыбеков А., Абугалиева С., Дидоренко С., Рсалиев А., Мауленбай А., Фанг Ч., Туруспеков Е., 20232023Затыбеков А., Абугалиева С., Дидоренко С., Рсалиев А., Мауленбай А., Фанг Ч., Туруспеков Е.Zatybekov A., Abugalieva S., Didorenko S., Rsaliyev A., Maulenbay A., Fang C., Turuspekov Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3927

Пепельная гниль, вызываемая грибным патогеном Macrophomina phaseolina, представляет собой опасное заболевание, поражающее сою (Glycine max (L.) Merrill.) во всем мире. Выявление генетических факторов, связанных с устойчивостью к пепельной гнили, имеет важное значение для создания устойчивых к болезням сортов сои. Мы провели полногеномный анализ ассоциации (ПГАА) с использованием различных моделей и генотипических данных, чтобы найти генетические детерминанты, лежащие в основе устойчивости сои к пепельной гнили. В исследовании использовали коллекцию, состоящую из 252 образцов сои, включая коммерческие сорта и селекционные линии, для выявления генетических вариаций, связанных с устойчивостью. Фенотипическую оценку проводили в естественных условиях в период 2021–2022 гг. В работе регистрировали уровень заболевания и показатели выживаемости для количественной оценки уровней устойчивости образцов. Генотипические данные состояли из двух наборов: результаты генотипирования с применением технологии Illumina iSelect 6K SNP, и данные полногеномного ресеквенирования. Полногеномный анализ ассоциации был выполнен с помощью четырех различных моделей (MLM, MLMM, FarmCPU и BLINK) на платформе GAPIT. В результате были идентифицированы SNP-маркеры 11 локусов количественных признаков, ассоциированных с устойчивостью к пепельной гнили. Гены-кандидаты в пределах идентифицированных геномных областей были изучены на предмет их функциональной аннотации и потенциальной роли в защитных реакциях растений. Результаты этого исследования могут внести дополнительный вклад в разработку стратегий молекулярной селекции для повышения устойчивости сортов сои к пепельной гнили. Маркер-опосредованный отбор может быть эффективно применен для ускорения процесса селекции, что позволит создавать сорта с повышенной устойчивостью к пепельной гнили. Использование устойчивых сортов может значительно сократить потери урожая и повысить устойчивость производства сои, что принесет пользу фермерам и обеспечит стабильное производство этой ценной культуры.

Charcoal rot (CR) caused by the fungal pathogen Macrophomina phaseolina is a devastating disease affecting soybean (Glycine max (L.) Merrill.) worldwide. Identifying the genetic factors associated with resistance to charcoal rot is crucial for developing disease-resistant soybean cultivars. In this research, we conducted a genome-wide association study (GWAS) using different models and genotypic data to unravel the genetic determinants underlying soybean resistance to сharcoal rot. The study relied on a panel of 252 soybean accessions, comprising commercial cultivars and breeding lines, to capture genetic variations associated with resistance. The phenotypic evaluation was performed under natural conditions during the 2021–2022 period. Disease severity and survival rates were recorded to quantify the resistance levels in the accessions. Genotypic data consisted of two sets: the results of genotyping using the Illumina iSelect 6K SNP (single-nucleotide polymorphism) array and the results of whole-genome resequencing. The GWAS was conducted using four different models (MLM, MLMM, FarmCPU, and BLINK) based on the GAPIT platform. As a result, SNP markers of 11 quantitative trait loci associated with CR resistance were identified. Candidate genes within the identified genomic regions were explored for their functional annotations and potential roles in plant defense responses. The findings from this study may further contribute to the development of molecular breeding strategies for enhancing CR resistance in soybean cultivars. Marker-assisted selection can be efficiently employed to accelerate the breeding process, enabling the development of cultivars with improved resistance to сharcoal rot. Ultimately, deploying resistant cultivars may significantly reduce yield losses and enhance the sustainability of soybean production, benefiting farmers and ensuring a stable supply of this valuable crop.

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The authors declare that there are no conflicts of interest present.