vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJGB-23-73vavilov-3933Research ArticleСЕЛЕКЦИЯ РАСТЕНИЙ НА ПРОДУКТИВНОСТЬ И КАЧЕСТВОPLANT BREEDING FOR PERFORMANCE AND QUALITYПребридинговые исследования устойчивой к листовой ржавчине Triticum aestivum/T. timopheevii линии Л624Prebreeding studies of leaf rust resistant Triticum aestivum/T. timopheevii line L624https://orcid.org/0000-0001-8324-9765СибикеевС. Н.SibikeevS. N.

Саратов

Saratov

sibikeev_sergey@mail.ruhttps://orcid.org/0000-0002-8460-6119АдонинаИ. Г.AdoninaI. G.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-3968-2470ДружинА. Е.DruzhinA. E.

Саратов

Saratov

https://orcid.org/0000-0001-9439-2102БарановаО. А.BaranovaO. A.

Пушкин, Санкт-Петербург

Pushkin

Федеральный аграрный научный центр Юго-ВостокаРоссияFederal Center of Agriculture Research of the South-East RegionRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Курчатовский геномный центр ИЦиГ СО РАНРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Kurchatov Genomic Center of ICG SB RASRussian FederationВсероссийский научно-исследовательский институт защиты растенийРоссияAll-Russian Research Institute of Plant ProtectionRussian Federation202301112023276623632Copyright © Сибикеев С.Н., Адонина И.Г., Дружин А.Е., Баранова О.А., 20232023Сибикеев С.Н., Адонина И.Г., Дружин А.Е., Баранова О.А.Sibikeev S.N., Adonina I.G., Druzhin A.E., Baranova O.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/3933

Вид Triticum timopheevii Zhuk. привлекает внимание селекционеров мягкой пшеницы высоким иммунитетом к возбудителю листовой ржавчины. Однако интрогрессии от этого вида в T. aestivum L. мало используются в практической селекции. В представленном исследовании изучена агрономическая ценность T. aestivum/T. timopheevii линии Л624 по сравнению с родительскими сортами Саратовская 68, Добрыня и сортом-стандартом Фаворит в течение 2017–2022 гг. Интрогрессии от T. timopheevii у Л624 выявлены с помощью метода FISH с зондами pSc119.2, pAs1 и Spelt1, а также микросателлитных маркеров Xgwm312, Xgpw4480 и Xksum73. Обнаружены транслокации 2AS.2AL-2AtL и в длинном плече хромосомы 2D. Линия Л624 высокоустойчива к Puccinia triticina как на фоне естественной эпифитотии, так и в лабораторных условиях. С использованием ПЦР-анализа с ДНК-маркером гена LrTt1 (Xgwm312) установлена его неидентичность Lr-гену(ам) у ли нии Л624. По данным пятилетнего изучения, урожайность зерна у Л624 была в среднем выше, чем у сортов Фаворит и Добрыня, но ниже, чем у сорта Саратовская 68. По массе 1000 зерен Л624 уступала реципиентам и была одного уровня с сортом-стандартом Фаворит. Интрогрессии от T. timopheevii у Л624 увеличили содержание белка в зерне по сравнению с сортами Саратовская 68 и Фаворит, но с сортом Добрыня оно было на одном уровне. В целом по показателям качества муки и хлеба линия Л624 не уступила сортам-реципиентам, а по объему и пористости хлеба превзошла Саратовскую 68. В то же время Л624 превышала сорт-стандарт Фаворит по упругости теста, отношению упругости теста к растяжимости и силе муки. Таким образом, полученные результаты позволяют сделать предположение, что интрогрессии в хромосомах 2А и 2D у линии Л624 не ухудшают хлебопекарные свойства.

Triticum timopheevii Zhuk. attracts the attention of bread wheat breeders with its high immunity to the leaf rust pathogen. However, introgressions from this species in Triticum aestivum L. are little used in practical breeding. In the presented study, the agronomic value of T. aestivum/T. timopheevii line L624 was studied in comparison with the parent cultivars Saratovskaya 68, Dobrynya and the standard cultivar Favorit during 2017–2022. Introgressions from T. timopheevii in L624 were detected by the FISH method with probes pSc119.2, pAs1 and Spelt1, as well as micro satellite markers Xgwm312, Xgpw4480 and Xksum73. Translocations of 2AS.2AL-2AtL and on 2DL were detected as well. Line L624 is highly resistant to Puccinia triticina both under the background of natural epiphytotics and under laboratory conditions. PCR analysis with the DNA marker of the LrTt1 gene (Xgwm312) revealed that it is not identical to the Lr gene(s) in L624. According to a five-year study, the grain yield of L624 was, on average, higher than that of Favorit and Dobrynya, but lower than that of Saratovskaya 68. Line L624 had a lower weight of 1000 grains than the recipients, and was at the same level with the standard cultivar Favorit. Introgressions from T. timopheevii in L624 increased the grain protein content by comparison with Saratovskaya 68 and Favorit, but it was at the same level as in Dobrynya. As for parameters of flour and bread, L624 was not inferior to the recipient cultivars, but by volume and porosity of bread, it surpassed Saratovskaya 68. Moreover, L624 surpassed Favorit by the elasticity of the dough, the ratio of the elas ticity of the dough to the extensibility and the strength of the flour. Thus, the results obtained suggest that introgressions in chromosomes 2A and 2D in L624 do not impair baking properties.

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The authors declare that there are no conflicts of interest present.