References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJGB-23-117

vavilov-4013

Research Article

АКТУАЛЬНЫЕ ТЕХНОЛОГИИ В ГЕНЕТИКЕ И СЕЛЕКЦИИ РАСТЕНИЙ

MAINSTREAM TECHNOLOGIES IN PLANT GENETICS AND BREEDING

Изучение потенциала исходного селекционного материала пшеницы (Triticum aestivum L.) в андрогенезе (Triticum aestivum L.)

Study of wheat (Triticum aestivum L.) breeding material potential for (Triticum aestivum L.)androgenesis

https://orcid.org/0000-0002-7499-6803

Петраш

Н. В.

Petrash

N. V.

Новосибирск

Novosibirsk

pnv11@bionet.nsc.ru

https://orcid.org/0000-0003-1573-1618

Капко

Т. Н.

Kapko

T. N.

Новосибирск

Novosibirsk

https://orcid.org/0000-0003-4497-9137

Советов

В. В.

Sovetov

V. V.

Новосибирск

Novosibirsk

Сибирский научно-исследовательский институт растениеводства и селекции – филиал Федерального исследовательского центра Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияSiberian Research Institute of Plant Production and Breeding – Branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian Federation

2023

29122023

27810221030

2023

Петраш Н.В., Капко Т.Н., Советов В.В.

Petrash N.V., Kapko T.N., Sovetov V.V.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/4013

Создание удвоенных гаплоидов – ценный биотехнологический подход в селекции растений, позволяющий ускоренно создавать новые сорта за счет одноэтапного получения гомозиготных линий. Целью настоящего исследования было проведение оценки показателей андрогенеза in vitro в культуре пыльников исходного селекционного материала сортов и комбинаций F1 и F2 и выявление перспективных образцов с хорошей отзывчивостью. В работе использован растительный материал, перспективный для селекционных программ Сибирского научно-исследовательского института растениеводства и селекции – филиала ИЦиГ СО РАН. Десять сортов мягкой пшеницы и гибриды F1 и F2 девяти комбинаций скрещивания оценивали по основным параметрам андрогенеза in vitro: числу новообразований, числу альбиносов и зеленых растений-регенерантов и всех регенерировавших растений. Индукцию андрогенеза in vitro проводили в культуре пыльников на питательной среде Chu (N6), в качестве регулятора роста использовали 1 мг/л 2.4-Д. У изучаемых образцов обнаружен различный ответ на индукцию андрогенеза in vitro. Отмечен максимальный выход новообразований у гибридов F2 Новосибирская 15 × Лютесценс ШТ-335. Наибольшее количество зеленых растений-регенерантов обнаружено у F1 Новосибирская 15 × Лютесценс ШТ-335. По результатам дисперсионного анализа установлено достоверное ( p < 0.01) влияние генотипа на изучаемые признаки. Выявлены сорта с хорошей отзывчивостью в культуре пыльников (Новосибирская 15) и с отсутствием отзывчивости к андрогенезу in vitro (Новосибирская 31). Сорт Новосибирская 16 характеризовался низкой регенерационной способностью новообразований. Среди гибридов значительный гетерозисный эффект отмечен по признаку «число новообразований на 100 пыльников» в комбинациях Новосибирская 15 × Лютесценс ШТ-335, Новосибирская 15 × Лютесценс 111/09, Загора Новосибирская × Обская 2. Сорт Новосибирская 15 рекомендован к включению в скрещивания как сорт, обеспечи вающий высокую отзывчивость в андрогенезе in vitro гибридов. Применение технологии удвоенн ых гаплоидов позволило быстро создать DH-линии на основе изучаемого материала.

Doubled haploid technology is a valuable biotechnological approach in plant breeding that enables one to quickly create new varieties through the single-stage production of homozygous lines. The aim of this study was to assess the indicators of in vitro androgenesis in the anther culture of the initial breeding material of varieties and combinations of F1 and F2 and to identify promising accessions with good responsiveness. For that purpose, the plant material that proved promising for the breeding programs of Siberian Research Institute of Plant Production and Breeding (SibRIPP&B) was used. Ten cultivars of common wheat and the F1 and F2 hybrids of nine combinations were evaluated for the main parameters of in vitro androgenesis such as the number of new formations, albino, green and all regenerated plants. Induction of androgenesis in vitro was carried out in anther culture in growth medium Chu (N6) containing 1 mg/l of growth regulator 2,4-D. The studied samples showed different responses to induction. The maximum level of new formations was found in F2 hybrids Novosibirskaya 15 × Lutescens ShT-335. The largest number of green plants was found in F1 Novosibirskaya 15 × Lutescens ShT-335. According to the results of variance analysis, a significant ( p < 0.01) influence of genotype on the studied traits was established. Varieties with good responsiveness to anther culture (Novosibirskaya 15) and lack of responsiveness to in vitro androgenesis (Novosibirskaya 31) were identified. Novosibirskaya 16 was characterized by a low regeneration capacity of new formations. A significant heterotic effect was revealed considering the number of new formations per 100 anthers among the hybrids of such combinations as Novosibirskaya 15 × Lutescens ShT-335, Novosibirskaya 15 × Lutescens 111/09, and Zagora Novosibirskaya × Obskaya 2. Novosibirskaya 15 was recommended for inclusion in crossings as a parental form that provides high hybrid responsiveness during in vitro androgenesis. The use of doubled haploid technology made it possible to quickly create DH-lines based on the breeding material.

удвоенные гаплоидыандрогенез in vitroкультура пыльниковTriticum aestivum L.гетерозисный эффект

doubled haploidsin vitro androgenesisanther cultureTriticum aestivum L.heterosis

This work was supported by Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences budget project No. FWNR-2022-0017.

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The authors declare that there are no conflicts of interest present.