vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-24-10vavilov-4058Research ArticleГЕНЕТИКА ЧЕЛОВЕКАHUMAN GENETICSАссоциации CAG-полиморфизма гена андрогенового рецептора с уровнем стероидных гормонов и антропометрическими показателями у мужчин: роль этнического фактораAssociations of CAG repeat polymorphism in the androgen receptor gene with steroid hormone levels and anthropometrics among men: the role of the ethnic factorhttps://orcid.org/0000-0002-7597-9204ОсадчукЛ. В.OsadchukL. V.

Новосибирск

Novosibirsk

losadch@bionet.nsc.ruhttps://orcid.org/0000-0003-0929-6832ВасильевГ. В.VasilievG. V.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-4210-7354ОсадчукА. В.OsadchukA. V.

Новосибирск

Novosibirsk

Федеральный исследовательский центр «Институт цитологии и генетики Сибирского отделения Российской академии наук»РоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian Federation2024020320242818089Copyright © Осадчук Л.В., Васильев Г.В., Осадчук А.В., 20242024Осадчук Л.В., Васильев Г.В., Осадчук А.В.Osadchuk L.V., Vasiliev G.V., Osadchuk A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4058

Андрогены необходимы для роста и поддержания гомеостаза костной ткани. Физиологические функции андрогенов опосредованы андрогеновым рецептором (АR). Ген андрогенового рецептора АR имеет полиморфный тринуклеотидный CAG­-повтор, и длина AR CAG-­повторов, регулируя чувствительность костной ткани к андрогенам, оказывает влияние на формирование скелета и пропорций тела. Цель исследования – выявить ассоциации между длиной АR CAG-­повторов, гормональными и антропометрическими показателями у мужчин различных этнических групп. В популяционном исследовании приняли участие мужчины добровольцы (n = 1078) трех этнических групп (славян, бурят и якутов) из городских популяций России. У участников измеряли рост, размах рук, длину ноги, длину второго и четвертого пальцев обеих рук, рассчитывали ростовые индексы: отношение роста к длине ноги, отношение размаха рук к росту, отношение длины второго к четвертому пальцу. Тестостерон и эстрадиол в образцах периферической крови определяли иммуноферментным методом. Генотипирование AR CAG­-повторов проводили с помощью фрагментного анализа и капиллярного электрофореза.

Установлены более высокие антропометрические показатели у славян по сравнению с бурятами и в большинстве случаев с якутами. Уровень тестостерона повышен у славян по сравнению с бурятами, но не отличался от якутов, а уровень эстрадиола понижен по сравнению с таковым у бурят или якутов, которые не отличались по этому показателю. Длина AR CAG-повторов составила 23, 24 и 25 триплетов (медианы) у славян, бурят и якутов соответственно. Выявлены положительные корреляции между длиной AR CAG-повторов и уровнем эстрадиола у бурят и уровнем тестостерона у якутов, причем длинные CAG-­повторы сопровождались повышенным уровнем эстрадиола у бурят и тестостерона у славян и якутов. Установлены этнозависимые корреляции между уровнем стероидных гормонов и антропометрическими показателями у всех этносов. Полученные данные предполагают существование этнозависимых ассоциаций AR CAG­-полиморфизма с размерами костей скелета, которые опосредуются стероидными гормонами как важными регуляторами роста и гомеостаза костной ткани.

Androgens are required for stimulation and maintenance of skeletal growth and bone homeostasis. Physiological functions of androgens are mediated through the androgen receptor (AR). The androgen receptor gene AR has a polymorphic trinucleotide CAG repeat and the length of AR CAG repeats determining the sensitivity of bone tissue to androgens is associated with skeleton formation and body proportions. This study aimed to investigate the relationship between AR CAG repeat polymorphism, circulating sex steroid hormones and the anthropometrics in males of different ethnic origins. Male volunteers of three ethnic groups (Slavs, Buryats, Yakuts) from urban Russian populations were recruited in a population­based study (n = 1078). Anthropometric indicators (height, arm span, leg length, the length of 2 and 4 digits of both hands) were measured and the following anthropometric indices were calculated: the ratio of height to leg length, the ratio of arm span to height, the ratio of lengths of second to fourth digit of the hand. Serum testosterone and estradiol were determined by enzyme immunoassay. Genotyping of the AR CAG repeats was performed using fragment analysis and capillary electrophoresis. Ethnic differences in all anthropometric and hormonal indicators have been established, with higher anthropometric indicators in Slavs than Buryats, and in most cases higher than in Yakuts. The testosterone level was higher among Slavs compared to Buryats, but did not differ from Yakuts; the estradiol level was lower among Slavs compared to Buryats, who did not differ from Yakuts. Buryats and Yakuts had a higher number of CAG repeats than Slavs (medians: Slavs, 23; Buryats, 24; Yakuts, 25). Positive correlations were found between the length of AR CAG repeats and estradiol levels in Buryats and testosterone levels in Yakuts, while longer CAG repeats were accompanied by higher estradiol levels in Buryats and testosterone levels in Slavs and Yakuts. Ethnic-specific correlations have been established between the steroid hormone levels and some anthropometric indicators in all ethnic groups. Available data suggest that the ethnic-­specific associations of AR CAG repeats with anthropometrics can be mediated by sex steroid hormones as important regulators of skeletal growth and bone homeostasis.

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The authors declare that there are no conflicts of interest present.