vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-24-19vavilov-4086Research ArticleИММУНИТЕТ РАСТЕНИЙ К БОЛЕЗНЯМPLANT IMMUNITYИзучение генетической коллекции земляники (Fragaria L.) по устойчивости к мучнистой росеStudy of a genetic collection of strawberry (Fragaria L.) for resistance to powdery mildewЛыжинА. С.LyzhinA. S.

Мичуринск

Michurinsk

Ranenburzhetc@yandex.ruЛукъянчукИ. В.Luk’yanchukI.  V.

Мичуринск

Michurinsk

Федеральный научный центр им. И.В. МичуринаРоссияI.V. Michurin Federal Scientific CenterRussian Federation202411042024282166174Copyright © Лыжин А.С., Лукъянчук И.В., 20242024Лыжин А.С., Лукъянчук И.В.Lyzhin A.S., Luk’yanchuk I.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4086

Мучнистая роса (Sphaerotheca macularis Mag. (син. Podosphaera aphanis Wallr.)) – опасное заболевание земляники (Fragaria L.). Устойчивость земляники к мучнистой росе контролируется полигенно. У различных сортов земляники садовой идентифицировано несколько генетических локусов (QTL) с большим вкладом в устойчивость к болезни. Для QTL 08 To-f разработаны диагностические ДНК-маркеры, показавшие высокий уровень надежности выявления гена в картирующих популяциях. Цель настоящего исследования – изучение генетической коллекции земляники по устойчивости к мучнистой росе и идентификация перспективных для селекции на устойчивость к S. macularis форм. Объектами исследования были дикорастущие виды рода Fragaria L., сорта и отборные сеянцы земляники садовой (F. × ananassa Duch.) селекции Федерального научного центра им. И.В. Мичурина, а также формы, интродуцированные из различных эколого-географических регионов. Для идентификации QTL 08 To-f использовали маркеры IB535110 и IB533828. Локус 08 To-f обнаружен у 23.2 % генотипов земляники, в том числе у дикорастущих видов F. moschata и F. orientalis, сортов земляники садовой отечественной (Былинная, Сударушка) и зарубежной (Florence, Korona, Malwina, Ostara, Polka, Red Gauntlet) селекции. Степень корреляции между наличием маркеров IB535110 и IB533828 и фенотипической устойчивостью (признаки поражения мучнистой росой отсутствуют) составила 0.649. Коэффициент детерминации (R2 ), показывающий вклад изучаемого локуса в формирование признака, равен 0.421, т. е. в 42.1 % случаев устойчивость определяется наличием QTL 08 To- f, тогда как в 57.9 % случаев влияние оказывают факторы внешней среды. Все генотипы земляники с локусом 08 To-f характеризуются высокой полевой устойчивостью к S. macularis в условиях г. Мичуринска Тамбовской области. Таким образом, локус 08 To-f является перспективным для придания устойчивости к местным расам мучнистой росы, а маркеры IB535110 и IB533828 могут быть использованы в программах маркер-опосредованной селекции по созданию устойчивых к мучнистой росе генотипов земляники.

Powdery mildew (Sphaerotheca macularis Mag. (syn. Podosphaera aphanis Wallr.)) is a dangerous disease of strawberry (Fragaria L.). The resistance of strawberry to powdery mildew is controlled polygenically. Several genetic loci with a large contribution to disease resistance have been identified in various strawberry varieties. Diagnostic DNA markers have been developed for QTL 08 To-f. They showed a high level of reliable gene detection in mapping populations. The purpose of this study was assessment of a strawberry genetic collection for resistance to powdery mildew and identification of promising strawberry forms for breeding for resistance to S. macularis. The objects of the study were wild species of the genus Fragaria L., varieties and selected seedlings of strawberry (Fragaria × ananassa Duch.) created in the I.V. Michurin Federal Scientific Center, and strawberry varieties introduced from various ecological and geographical regions. To identify QTL 08 To-f, DNA markers IB535110 and IB533828 were used. Locus 08 To-f was detected in 23.2 % of the analyzed strawberry genotypes, including wild species F. moschata and F. orientalis, strawberry varieties of Russian breeding (Bylinnaya and Sudarushka) and foreign breeding (Florence, Korona, Malwina, Ostara, Polka and Red Gauntlet). The correlation between the presence of markers IB535110 and IB533828 and phenotypic resistance (powdery mildew effect on strawberry plants is absent) was 0.649. The determination coefficient (R2 ) showing the contribution of the studied locus to the manifestation of the trait was 0.421, that is, in 42.1 % of cases resistance was explained by the presence of QTL 08 To-f, and in 57.9 % of cases, by other genetic factors. All strawberry genotypes with locus 08 To-f were characterized by high field resistance to S. macularis in the conditions of Michurinsk, Tambov region. Thus, locus 08 To-f is promising for conferring resistance on local powdery mildew races, and markers IB535110 and IB533828 can be used in marker-assisted breeding programs to create powdery mildewresistant strawberry genotypes.

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The authors declare that there are no conflicts of interest present.