vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-24-22vavilov-4088Research ArticleГЕНЕТИКА ЖИВОТНЫХANIMAL GENETICSРазличия в плодовитости между двумя линиями Drosophila melanogaster дикого типа коррелируют с различиями в экспрессии гена Jheh, кодирующего фермент деградации ювенильного гормонаFertility differences between two wild-type Drosophila melanogaster lines correlate with differences in the expression of the Jheh gene, which codes for an enzyme degrading juvenile hormoneАндреенковаО. В.AndreenkovaO. V.

Новосибирск

Novosibirsk

АдоньеваН. В.AdonyevaN. V.

Новосибирск

Novosibirsk

ЕфимовВ. М.EfimovV. M.

Новосибирск

Novosibirsk

ГрунтенкоН. Е.GruntenkoN.  E.

Новосибирск

Novosibirsk

nataly@bionet.nsc.ruФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian Federation202411042024282185189Copyright © Андреенкова О.В., Адоньева Н.В., Ефимов В.М., Грунтенко Н.Е., 20242024Андреенкова О.В., Адоньева Н.В., Ефимов В.М., Грунтенко Н.Е.Andreenkova O.V., Adonyeva N.V., Efimov V.M., Gruntenko N.E.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4088

Ювенильный гормон играет у личинок Drosophila melanogaster роль “status quo” гормона, препятствуя преждевременному наступлению метаморфоза, а у имаго выполняет гонадотропную функцию, обеспечивая подготовку яичников к вителлогенезу. При снижении уровня ювенильного гормона у самок D. melanogaster наблюдаются нарушения репродукции, выражающиеся в задержке начала откладки яиц и снижении плодовитости. Еще одним фактором, способным повлиять на репродуктивную функцию насекомых, является инфицирование матерински наследуемой симбиотической α-протеобактерией Wolbachia. Настоящее исследование посвящено анализу экспрессии двух генов метаболизма ювенильного гормона, кодирующих ферменты его синтеза и деградации – кислую О-метилтрансферазу ювенильного гормона (jhamt) и эпоксидгидразу ювенильного гормона (Jheh1) соответственно, у четырех линий D. melanogaster дикого типа, две из которых инфицированы Wolbachia. Линии w153 и Bi90 происходят от отдельных самок, отловленных в дикой природе, и инфицированы Wolbachia, а линии w153T и Bi90T получены на их основе посредством тетрациклиновой обработки и характеризуются отсутствием инфекции. Известно, что линия Bi90 инфицирована штаммом Wolbachia генотипа wMel, а линия w153 – штаммом Wolbachia wMelPlus, относящимся к генотипу wMelCS. Обнаружено, что инфицирование как одним, так и другим штаммом Wolbachia не влияет на экспрессию исследованных генов. В то же время показано, что обе линии с ядерным генотипом w153 отличаются от линий с генотипом Bi90 повышенным уровнем экспрессии гена Jheh1 и не различаются по уровню экспрессии гена jhamt. Уровень плодовитости не зависел от инфицирования Wolbachia, но у линий с ядерным генотипом w153 он оказался существенно ниже, чем у линий с ядерным генотипом Bi90. Полученные данные позволяют сделать обоснованное предположение о том, что межлинейный полиморфизм D. melanogaster по метаболизму ювенильного гормона коррелирует с уровнем плодовитости и определяется деградацией гормона, а не его синтезом.

Juvenile hormone plays a “status quo” role in Drosophila melanogaster larvae, preventing the untimely metamorphosis, and performs a gonadotropic function in imagoes, ensuring the ovaries’ preparedness for vitellogenesis. The decreased level of juvenile hormone results in reproductive disorders in D. melanogaster females including a delay in the oviposition onset and a fertility decrease. Another factor that can affect the insect reproduction is an infection with the maternally inherited symbiotic α-proteobacterium Wolbachia. The present study is devoted to the analysis of the expression of two juvenile hormone metabolism genes encoding enzymes of its synthesis and degradation, juvenile hormone acid O-methyltransferase ( jhamt) and juvenile hormone epoxide hydrase (Jheh1), respectively, in four wild-type D. melanogaster lines, two of them being infected with Wolbachia. Lines w153 and Bi90 were both derived from an individual wild-caught females infected with Wolbachia, while lines w153T and Bi90T were derived from them by tetracycline treatment and are free of infection. Line Bi90 is known to be infected with the Wolbachia strain wMel, and line w153, with the Wolbachia strain wMelPlus belonging to the wMelCS genotype. It was found that infection with either Wolbachia strain does not affect the expression of the studied genes. At the same time, it was shown that the w153 and w153T lines differ from the Bi90 and Bi90T lines by an increased level of the Jheh1 gene expression and do not differ in the jhamt gene expression level.  Analysis of the fertility of these four lines showed that it does not depend on Wolbachia infection either, but differs between lines with different nuclear genotypes: in w153 and w153T, it is significantly lower than in lines Bi90 and Bi90T. The data obtained allow us to reasonably propose that the inter-line D. melanogaster polymorphism in the metabolism of the juvenile hormone is determined by its degradation (not by its synthesis) and correlates with the fertility level.

Drosophila melanogasterWolbachiajhamtJheh1экспрессия геновплодовитостьметаболизм ювенильного гормонаDrosophila melanogasterWolbachiajhamtJheh1gene expressionfertilityjuvenile hormone metabolismThe work was done with the support of the Russian Science Foundation grant No. 21-14-00090. The maintenance of experimental D. melanogaster lines was carried out in the Drosophila collection of the Institute of Cytology and Genetics SB RAS and was supported by BP #FWNR-2022-0019 of the Ministry of Science and Higher Education of the Russian Federation. Acknowledgements. The authors thank Darya Kochetova for the translation of the article.ReferencesAdonyeva N.V., Menshanov P.N., Gruntenko N. A link between atmospheric pressure and fertility of Drosophila laboratory strains. Insects. 2021;12(10):947. DOI 10.3390/insects12100947Adonyeva N.V., Menshanov P.N., Gruntenko N. A link between atmospheric pressure and fertility of Drosophila laboratory strains. Insects. 2021;12(10):947. 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The authors declare that there are no conflicts of interest present.