vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-24-61vavilov-4236Research ArticleУСТОЙЧИВОСТЬ РАСТЕНИЙ К СТРЕССОВЫМ ФАКТОРАМRESISTANCE OF PLANTS TO STRESS FACTORSРаспространенность и видовой состав вирусов картофеля в Новосибирской областиDistribution and species composition of potato viruses in the Novosibirsk regionМасленниковаВ. С.MaslennikovaV. S.

Новосибирск

Novosibirsk

ПыхтинаМ. Б.PykhtinaM. B.

Новосибирск

Novosibirsk

pykhtina_maria@mail.ruТабанюховК. А.TabanyukhovK. A.

Новосибирск

Novosibirsk

ШелиховаЕ. В.ShelikhovaE. V.

Новосибирск

Novosibirsk

МосалевК. И.MosalevK. I.

Новосибирск

Novosibirsk

КатохинА. В.KatokhinA. V.

Новосибирск

Novosibirsk

https://orcid.org/0000-0001-9181-0487БондарьА. А.BondarA. A.

Новосибирск

Novosibirsk

БеклемишевА. Б.BeklemishevA. B.

Новосибирск

Novosibirsk

https://orcid.org/0000-0001-9425-413XВоеводаМ. И.VoevodaM. I.

Новосибирск

Novosibirsk

Федеральный исследовательский центр фундаментальной и трансляционной медицины; Новосибирский государственный аграрный университетРоссияFederal Research Center of Fundamental and Translational Medicine; Novosibirsk State Agrarian UniversityRussian FederationФедеральный исследовательский центр фундаментальной и трансляционной медициныРоссияFederal Research Center of Fundamental and Translational MedicineRussian FederationИнститут химической биологии и фундаментальной медицины Сибирского отделения Российской академии наукРоссияInstitute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of SciencesRussian Federation202402092024285554562Copyright © Масленникова В.С., Пыхтина М.Б., Табанюхов К.А., Шелихова Е.В., Мосалев К.И., Катохин А.В., Бондарь А.А., Беклемишев А.Б., Воевода М.И., 20242024Масленникова В.С., Пыхтина М.Б., Табанюхов К.А., Шелихова Е.В., Мосалев К.И., Катохин А.В., Бондарь А.А., Беклемишев А.Б., Воевода М.И.Maslennikova V.S., Pykhtina M.B., Tabanyukhov K.A., Shelikhova E.V., Mosalev K.I., Katokhin A.V., Bondar A.A., Beklemishev A.B., Voevoda M.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4236

Среди множества болезней, поражающих растения картофеля, именно вирусные инфекции являются наиболее распространенными и наносят значительный ущерб хозяйствам, влияя как на урожайность, так и на качество картофеля. В связи с этим важное условие сохранения семенного фонда картофеля в России – систематический мониторинг и раннее высокоспецифичное обнаружение вирусных инфекций картофеля. Целью работы было исследование образцов сортов картофеля, собранных на территории Новосибирской области (НСО), на наличие вирусных инфекций методом ОТ-ПЦР. Изучено 130 растений картофеля из четырех районов Новосибирской области. В результате мониторинга обнаружены следующие вирусы: PVY (potato virus Y), PVS (potato virus S), PVM (potato virus M) и PVХ (potato virus X). Ни в одном из анализируемых образцов не найден карантинный объект – вироид веретеновидности клубней картофеля (potato spindle tuber viroid – PSTVd). Максимальная частота встречаемости в районах области была отмечена для трех вирусов – PVY, PVM и PVS. Смешанные вирусные инфекции составили заметную долю образцов: встречаемость комбинации инфекции PVY + PVM в растениях составляла 25.0 %, PVY + PVS – 22.6 %. Для отработки методов выяснения штаммовой принадлежности изучаемых образцов проведено секвенирование нуклеотидных последовательностей капсидных белков 10 изолятов Y-вируса. С просеквенированными последовательностями был осуществлен филогенетический анализ совместно с набором последовательностей референсных штаммов 261-4, Eu-N, N:O, NE-11, NTNa, NTNb, N-Wi, O, O5, SYR_I, SYR_II, SYR_III, взятых в GenBank. В результате филогенетического анализа установлено, что образцы из НСО распределились в две группы штаммов: группа 1, включающая также изоляты референсных штаммов 261-4/SYR_III, и группа 2 – NTNa. Полученные результаты штаммовой принадлежности образцов из НСО закладывают основу для разработки ДНК- и иммуннодиагностических систем для выявления PVY, циркулирующих в НСО, а также для выяснения источника и путей проникновения конкретных штаммов вируса.

Among the many diseases that affect potato plants, viral infections are the most common and cause significant damage to farms, affecting both the yield and quality of potatoes. In this regard, an important condition for preserving the potato seed fund in Russia is systematic monitoring and early highly specific detection of potato viral infections. The purpose of the work is to study samples of potato varieties collected in the Novosibirsk region for the presence of viral infections using RT-PCR. 130 potato plants from three districts of the Novosibirsk region (NR) were studied. As a result of monitoring, the following viruses were identified: PVY (potato virus Y), PVS (potato virus S), PVM (potato virus M) and PVX (potato virus X). The quarantine pathogen potato spindle tuber viroid (PSTVd) was not detected in any of the samples analyzed. The maximum frequency of occurrence in the region was noted for three viruses: PVY, PVM and PVS. A significant proportion of the samples were mixed viral infections: the occurrence of the combination of infection PVY + PVM in plants was 25.0 %, and PVY + PVS, 22.6 %. To develop methods for determining the strain affiliation of the studied samples, the nucleotide sequences of the capsid protein genes of 10 Y-virus isolates were sequenced. Phylogenetic analysis of the studied sequences of NR isolates was carried out with a set of sequences of reference strains 261-4, Eu-N, N:O, NE-11, NTNa, NTNb, N-Wi, O, O5, SYR_I, SYR_II and SYR_III retrieved from GenBank. As a result of phylogenetic analysis, it was established that NR viral samples fell into two groups of strains: group 1, which also includes isolates of the reference strains 261-4/SYR_III, and group 2, NTNa. The obtained results of the strain affiliation of NR samples lay the basis for the development of DNA and immunodiagnostic systems for identifying PVY circulating in NR, as well as for elucidating the source and routes of entry of specific virus strains.

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The authors declare that there are no conflicts of interest present.