vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-24-84vavilov-4349Research ArticleГЕНЕТИКА ЖИВОТНЫХANIMAL GENETICSКомплексный цитогенетический анализ кариотипов трех видов уток (шилохвость, кряква и обыкновенный гоголь) и эволюция кариотипов у представителей семейства Anatidae (Anseriformes, Aves)Detailed cytogenetic analysis of three duck species (the northern pintail, mallard, and common goldeneye) and karyotype evolution in the family Anatidae (Anseriformes, Aves)https://orcid.org/0000-0002-9122-4143БеклемишеваВ. Р.BeklemishevaV. R.

Новосибирск

Novosibirsk

bekl@mcb.nsc.ruhttps://orcid.org/0000-0001-7358-3612ТишаковаК. В.TishakovaK. V.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-0951-5209РоманенкоС. А.RomanenkoS. A.

Новосибирск

Novosibirsk

АндреюшковаД. А.AndreushkovaD. A.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-9059-875XЮдкинВ. А.YudkinV. A.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-1148-6283ИнтересоваЕ. А.InteresovaE. А.

Новосибирск

Томск

Novosibirsk

Tomsk

https://orcid.org/0000-0002-3573-2354ЯнгФ.YangF.

Цзыбо

Zibo

https://orcid.org/0000-0001-9372-1381Фергюсон-СмитМ. А.Ferguson-SmithM. A.

Кембридж

Cambridge

https://orcid.org/0000-0002-8282-1085ГрафодатскийА. С.GraphodatskyA. S.

Новосибирск

Novosibirsk

https://orcid.org/0000-0003-3812-4853ПроскуряковаА. А.ProskuryakovaA. A.

Новосибирск

Novosibirsk

Институт молекулярной и клеточной биологии Сибирского отделения Российской академии наукРоссияInstitute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of SciencesRussian FederationИнститут систематики и экологии животных Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университетРоссияInstitute of Systematics and Ecology of Animals of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityRussian FederationИнститут систематики и экологии животных Сибирского отделения Российской академии наук; Томский государственный университетРоссияInstitute of Systematics and Ecology of Animals of the Siberian Branch of the Russian Academy of Sciences; Tomsk State UniversityRussian FederationШкола медицины и наук о жизни, Шандонский технологический университетКитайSchool of Life Sciences and Medicine, Shandong University of TechnologyChinaКембриджский ресурсный центр сравнительной геномики, Кембриджский университетВеликобританияCambridge Resource Center for Comparative Genomics, University of CambridgeUnited Kingdom202422112024287759769Copyright © Беклемишева В.Р., Тишакова К.В., Романенко С.А., Андреюшкова Д.А., Юдкин В.А., Интересова Е.А., Янг Ф., Фергюсон-Смит М.А., Графодатский А.С., Проскурякова А.А., 20242024Беклемишева В.Р., Тишакова К.В., Романенко С.А., Андреюшкова Д.А., Юдкин В.А., Интересова Е.А., Янг Ф., Фергюсон-Смит М.А., Графодатский А.С., Проскурякова А.А.Beklemisheva V.R., Tishakova K.V., Romanenko S.A., Andreushkova D.A., Yudkin V.A., Interesova E.А., Yang F., Ferguson-Smith M.A., Graphodatsky A.S., Proskuryakova A.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4349

Курообразные (Galliformes) и гусеобразные (Anseriformes) – две ветви группы Galloanserae, базальные по отношению к остальным Neognathae. В сравнении с Galliformes, эволюция кариотипов Anseriformesнедостаточно изучена. Настоящее исследование посвящено представителям гусеобразных и изменению иххромосомных наборов в ходе эволюции. Была получена подробная информация о кариотипах (G-, C-бэндинг,флуоресцентная гибридизация in situ) трех видов уток: шилохвости (Anas acuta, 2n = 80), кряквы (Anas platyrhynchos, 2n = 80) и обыкновенного гоголя (Bucephala clangula, 2n = 80). С использованием зондов, разработанных на основе сортированных хромосом авдотки (Burhinus oedicnemus, 2n = 42, Charadriiformes), были выявленырайоны гомологии на макрохромосомах и части микрохромосом уток. Изученные виды рода Anas имеют одинаковое число макрохромосом, при этом у B. clangula число крупных хромосом увеличено за счет двух разрывовпредковых элементов. В отличие от представителей Anas, у этого вида обнаружены массивные гетерохроматиновые блоки в большинстве крупных макроаутосом и в половых хромосомах. Данные хромосомного пэйнтингадополнены информацией о локализации рибосомной ДНК и амплификации теломерных повторов. Сравнительный анализ геномов с помощью приложения D-GENIES подтвердил высокий уровень консерватизма синтенныхгрупп у Anatidae. Полученные результаты расширили представление о преобразованиях макро- и половых хромосом Anseriformes в ходе эволюции.

Galliformes and Anseriformes are two branches of the Galloanserae group, basal to other Neognathae. In contrast to Galliformes, Anseriformes have not been thoroughly researched by cytogenetic methods. This report is focused on representatives of Anseriformes and the evolution of their chromosome sets. Detailed cytogenetic analysis (G-banding, C-banding, and fluorescence in situ hybridization) was performed on three duck species: the northern pintail (Anas acuta, 2n = 80), the mallard (A. platyrhynchos, 2n = 80), and the common goldeneye (Bucephala clangula, 2n = 80). Using stone curlew (Burhinus oedicnemus, 2n = 42, Charadriiformes) chromosome painting probes, we created homology maps covering macrochromosomes and some microchromosomes. The results indicated a high level of syntenic group conservation among the duck genomes. The two Anas species share their macrochromosome number, whereas in B. clangula, this number is increased due to fissions of two ancestral elements. Additionally, in this species, the presence of massive heterochromatic blocks in most macroautosomes and sex chromosomes was discovered. Localization of clusters of ribosomal DNA and telomere repeats revealed that the duck karyotypes contain some microchromosomes that bear ribosomal RNA genes and/or are enriched for telomere repeats and constitutive heterochromatin. Dot plot (D-GENIES) analysis confirmed the established view about the high level of syntenic group conservation among Anatidae genomes. The new data about the three Anatidae species add knowledge about the transformation of macro- and sex chromosomes of Anseriformes during evolution.

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The authors declare that there are no conflicts of interest present.