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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJ15.070</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-446</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>СТРУКТУРНАЯ КОМПЬЮТЕРНАЯ БИОЛОГИЯ И ФАРМАКОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Cytoplasmic heredity</subject></subj-group></article-categories><title-group><article-title>Влияние типов цитоплазматической мужской стерильности на содержание хлорофилла в листьях гибридов зернового сорго</article-title><trans-title-group xml:lang="en"><trans-title>Effect of cytoplasmic male sterility type on chlorophyll content in leaves of grain sorghum hybrids</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кибальник</surname><given-names>О. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Kibalnik</surname><given-names>O. P.</given-names></name></name-alternatives><email xlink:type="simple">kibalnik79@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Эльконин</surname><given-names>Л. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Elkonin</surname><given-names>L. A.</given-names></name></name-alternatives><email xlink:type="simple">lelkonin@gmail.com</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение Российский научно-исследовательский и проектно-технологический институт сорго и кукурузы «Россорго», Саратов, Россия<country>Россия</country></aff><aff xml:lang="en">All-Russia Research Institute for Sorghum and Maize “Rossorgo”, Saratov, Russia<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Научно-исследовательский институт сельского хозяйства Юго-Востока», Саратов, Россия<country>Россия</country></aff><aff xml:lang="en">Agricultural Research Institute for the South-East Region of Russia, Saratov, Russia<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2015</year></pub-date><pub-date pub-type="epub"><day>02</day><month>12</month><year>2015</year></pub-date><volume>19</volume><issue>5</issue><fpage>538</fpage><lpage>544</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кибальник О.П., Эльконин Л.А., 2015</copyright-statement><copyright-year>2015</copyright-year><copyright-holder xml:lang="ru">Кибальник О.П., Эльконин Л.А.</copyright-holder><copyright-holder xml:lang="en">Kibalnik O.P., Elkonin L.A.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/446">https://vavilov.elpub.ru/jour/article/view/446</self-uri><abstract><p>Эффективное использование новых типов цитоплазматической мужской стерильности в селекции гетерозисных гибридов сорго невозможно без знания их влияния на основные биологические и хозяйственно ценные признаки растений. Воздействие стерильных цитоплазм типов А3, А4, 9Е и М35-1А на содержание хлорофиллов a и b в разные фазы развития растений сорго исследовали у гибридов F1, полученных в результате скрещивания двух наборов изоядерных ЦМС-линий (с геномами Пищевого 614 и Желтозерного 10) с двумя опылителями (Пищевое 35 и Меркурий). Обнаружено, что разные типы ЦМС-индуцирующих цитоплазм различаются между собой по содержанию хлорофил- лов, при этом генотипы ЦМС-линии и опылителя влияют на проявление цитоплазматических различий. У гибридов, полу­ченных с использованием ЦМС-линий с геномом Пищевого 614, обнаружено, что стерильная цитоплазма М35-1А повышала содержание хлорофилла a, по сравнению с цитоплазмой 9Е. У гибридов, полученных с использованием ЦМС-линий с геномом Желто­зерного 10 и опылителя Пищевого 35, стерильная цитоплазма А4 повышала сумму хлорофиллов a и b, по сравнению с цито­плазмами А3 и 9Е, тогда как у гибридов, полученных при использовании опылителя Меркурий, различия между цитоплазмами не наблюдались. Выявлен эффект гетерозиса по содержанию хлорофиллов a и b в фазу кущения у гибридов F1 на основе ЦМС-линий с геномом Пищевого 614 на стерильных цитоплазмах типов М35-1А и 9Е. У гибридов на основе цитоплазмы М35-1А наблюдалось сверхдоминирование общего содержания хлорофиллов: истинный гетерозис превышал аналогичные показатели у гибридов на цитоплазме 9Е на 19,0 %, гипотетический – на 20,6 %. Данные показывают, что привлечение новых типов ЦМС-индуцирующих цитоплазм позволяет создавать гибриды с эффектом гетерозиса по содержа­нию хлорофилла и может быть использовано для повышения их продуктивности.</p></abstract><trans-abstract xml:lang="en"><p>New types of CMS-inducing cytoplasms cannot be applied to hybrid sorghum breeding without knowledge of their effects on major biological and commercial plant traits. In our studies of F1 hybrids obtained by crossing of two sets of isonuclear CMS lines (with nuclear genomes of cv. Pishchevoye 614 (P614) and Zhelyozyornoye 10 (Zh10)) to two pollen parents (cv. Pishchevoye 35 (P35) and Mercury), we focused our attention on the effects of A3, A4, 9E, and M35-1A cytoplasms on chlorophyll content at different developmental stages of sorghum plants. It was found that hybrids with different types of male-sterile cytoplasm differed in chlorophyll content, and the genotypes of the CMS line and the pollen parent influenced the manifestation of cytoplasmic differences. In the F1 hybrids obtained with CMS lines possessing the P614 genome, sterile M35-1A cytoplasm increased chlorophyll a content, in comparison to 9E cytoplasm. In the F1 hybrids obtained with CMS lines with the Zh10 genome and the P35 pollen parent, sterile A4 cytoplasm increased the sum of chlorophyll a and b, in comparison to A3 and 9E cytoplasms, whereas no differences were recorded in the F1 hybrids obtained with Mercury. The F1 hybrids obtained with CMS lines with the P614 genome showed heterosis for total chlorophyll content at the tillering stage. Overdominance of this trait was observed in hybrids with M35-1A cytoplasm; true heterosis exceeded analogous indices in 9E cytoplasm by 19.0 %, and the hypothetical heterosis, by 20.6 %. These data demonstrate that the application of new types of CMS-inducing cytoplasms allows raise of F1 hybrids with heterosis for chlorophyll content. Thus, such types can be used to increase hybrid productivity.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>сорго</kwd><kwd>гибриды F1</kwd><kwd>цитоплазматическая мужская стерильность</kwd><kwd>изоядерные линии</kwd><kwd>хлорофилл</kwd><kwd>гетерозис</kwd></kwd-group><kwd-group xml:lang="en"><kwd>grain sorghum</kwd><kwd>F1 hybrids</kwd><kwd>cytoplasmic male sterility</kwd><kwd>isonuclear lines</kwd><kwd>chlorophyll</kwd><kwd>heterosis</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>РФФИ</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Адрианова Ю.Е., Тарчевский И.А. Хлорофилл и продуктивность растений. М., 2000.</mixed-citation><mixed-citation xml:lang="en">Адрианова Ю.Е., Тарчевский И.А. Хлорофилл и продуктивность растений. М., 2000.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Гужов Ю.Л., Фукс А., Валичек П. Селекция и семеноводство культивируемых растений. М., 1999.</mixed-citation><mixed-citation xml:lang="en">Гужов Ю.Л., Фукс А., Валичек П. Селекция и семеноводство культивируемых растений. М., 1999.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Доспехов Б.А. Методика полевого опыта (с основами статистической обработки результатов исследований). М., 2011.</mixed-citation><mixed-citation xml:lang="en">Доспехов Б.А. Методика полевого опыта (с основами статистической обработки результатов исследований). М., 2011.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Ермаков А.И. Методы биохимических исследований растений. Л., 1987.</mixed-citation><mixed-citation xml:lang="en">Ермаков А.И. Методы биохимических исследований растений. Л., 1987.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Кибальник О.П., Эльконин Л.А. Влияние типов стерильных цитоплазм на содержание пигментов в листьях гибридов F1 зернового сорго. Докл. РАСХН. 2009;1:18-21.</mixed-citation><mixed-citation xml:lang="en">Кибальник О.П., Эльконин Л.А. Влияние типов стерильных цитоплазм на содержание пигментов в листьях гибридов F1 зернового сорго. Докл. РАСХН. 2009;1:18-21.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Шимкевич А.М., Макаров В.Н., Голоенко И.М., Давыденко О.Г. Функциональное состояние фотосинтетического аппарата у аллоплазматических линий ячменя. Экол. генетика. 2006;4(2): 37-42.</mixed-citation><mixed-citation xml:lang="en">Шимкевич А.М., Макаров В.Н., Голоенко И.М., Давыденко О.Г. Функциональное состояние фотосинтетического аппарата у аллоплазматических линий ячменя. Экол. генетика. 2006;4(2): 37-42.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Эльконин Л.А., Кожемякин В.В., Ишин А.Г. Использование новых ЦМС-индуцирующих цитоплазм для создания скороспелых линий сорго с мужской стерильностью. Докл. РАСХН. 1997;2:7-9.</mixed-citation><mixed-citation xml:lang="en">Эльконин Л.А., Кожемякин В.В., Ишин А.Г. Использование новых ЦМС-индуцирующих цитоплазм для создания скороспелых линий сорго с мужской стерильностью. Докл. РАСХН. 1997;2:7-9.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Юрина Н.П., Одинцова М.С. Сигнальные системы растений. Пластидные сигналы и их роль в экспрессии ядерных генов. Физиол. растений. 2007;54(4):485-498.</mixed-citation><mixed-citation xml:lang="en">Юрина Н.П., Одинцова М.С. Сигнальные системы растений. Пластидные сигналы и их роль в экспрессии ядерных генов. Физиол. растений. 2007;54(4):485-498.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Amiri Behzadi A., Satyavathi C., Singh S., Bharadwaj C., Singh M. Estimation of heterosis in diverse cytoplasmic male sterile sources of Pearl millet [Pennisetum glaucum (L.) R. Br.]. Ann. Agric. Res. 2012;33(4):220-227.</mixed-citation><mixed-citation xml:lang="en">Amiri Behzadi A., Satyavathi C., Singh S., Bharadwaj C., Singh M. Estimation of heterosis in diverse cytoplasmic male sterile sources of Pearl millet [Pennisetum glaucum (L.) R. Br.]. Ann. Agric. Res. 2012;33(4):220-227.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Aruna C., Shrotria P.K., Pahuja S.K., Umakanth A.V., Bhat B.V., Devender A.V., Patil J.V. Fodder yield and quality in forage sorghum: scope for improvement though diverse male sterile cytoplasms. Crop Pasture Sci. 2013;63(12):1114-1123. DOI: 10.1071/cp12215</mixed-citation><mixed-citation xml:lang="en">Aruna C., Shrotria P.K., Pahuja S.K., Umakanth A.V., Bhat B.V., Devender A.V., Patil J.V. Fodder yield and quality in forage sorghum: scope for improvement though diverse male sterile cytoplasms. Crop Pasture Sci. 2013;63(12):1114-1123. DOI: 10.1071/cp12215</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Blanco N.E., Guinea-Díaz M., Whelan J., Strand А. Interaction between plastid and mitochondrial retrograde signalling pathways during changes to plastid redox status. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 2014;369:1640. DOI: 10.1098/rstb.2013.0231</mixed-citation><mixed-citation xml:lang="en">Blanco N.E., Guinea-Díaz M., Whelan J., Strand А. Interaction between plastid and mitochondrial retrograde signalling pathways during changes to plastid redox status. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 2014;369:1640. DOI: 10.1098/rstb.2013.0231</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Bollivar D.W. Recent advances in chlorophyll biosynthesis. Photosynth. Res. 2006;89(3):1-22. DOI: 10.1007/s11120-006-9076-6</mixed-citation><mixed-citation xml:lang="en">Bollivar D.W. Recent advances in chlorophyll biosynthesis. Photosynth. Res. 2006;89(3):1-22. DOI: 10.1007/s11120-006-9076-6</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Chamola R., Balyan H., Bhat S. Effect of alien cytoplasm and fertility restorer genes on agronomic and physiological traits of Brassica juncea L. Czern. Plant Breeding. 2013;132(6):681-687. DOI: 10.1111/pbr.12080</mixed-citation><mixed-citation xml:lang="en">Chamola R., Balyan H., Bhat S. Effect of alien cytoplasm and fertility restorer genes on agronomic and physiological traits of Brassica juncea L. Czern. Plant Breeding. 2013;132(6):681-687. DOI: 10.1111/pbr.12080</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Chi W., Sun X., Zhang L. Intracellular signaling from plastid to nucleus. Annu. Rev. Plant Biology. 2013;64:559-582. DOI: 10.1146/annurev-arplant-050312-120147</mixed-citation><mixed-citation xml:lang="en">Chi W., Sun X., Zhang L. Intracellular signaling from plastid to nucleus. Annu. Rev. Plant Biology. 2013;64:559-582. DOI: 10.1146/annurev-arplant-050312-120147</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Delorme V., Keen C.L., Rai K.N., Leaver C.J. Cytoplasmic-nuclear male sterility in pearl millet: comparative RFLP and transcript analyses of isonuclear male-sterile lines. Theor. Appl. Genet. 1997;95(5):961-968. DOI: 10.1007/s001220050648</mixed-citation><mixed-citation xml:lang="en">Delorme V., Keen C.L., Rai K.N., Leaver C.J. Cytoplasmic-nuclear male sterility in pearl millet: comparative RFLP and transcript analyses of isonuclear male-sterile lines. Theor. Appl. Genet. 1997;95(5):961-968. DOI: 10.1007/s001220050648</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Eckhardt U., Grimm B., Hörtensteiner S. Recent advances in chlorophyll biosynthesis and breakdown in higher plants. Plant Mol. Biol. 2004;56(1):1-14. DOI: 10.1007/s11103-004-2331-3</mixed-citation><mixed-citation xml:lang="en">Eckhardt U., Grimm B., Hörtensteiner S. Recent advances in chlorophyll biosynthesis and breakdown in higher plants. Plant Mol. Biol. 2004;56(1):1-14. DOI: 10.1007/s11103-004-2331-3</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Elkonin L.A., Kozhemyakin V.V., Ishin A.G. Nuclear-cytoplasmic interactions in fertility restoration in sorghum: alternative CMS-inducing cytoplasms. Int. Sorghum Millet Newslett. 1995;36:75-76.</mixed-citation><mixed-citation xml:lang="en">Elkonin L.A., Kozhemyakin V.V., Ishin A.G. Nuclear-cytoplasmic interactions in fertility restoration in sorghum: alternative CMS-inducing cytoplasms. Int. Sorghum Millet Newslett. 1995;36:75-76.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Elkonin L.A., Kozhemyakin V.V., Ishin A.G. Comparative analysis of restoration of male-sterile (CMS)-inducing cytoplasms A3 and M35-1. Int. Sorghum Millet Newslett. 1997;38:29-30.</mixed-citation><mixed-citation xml:lang="en">Elkonin L.A., Kozhemyakin V.V., Ishin A.G. Comparative analysis of restoration of male-sterile (CMS)-inducing cytoplasms A3 and M35-1. Int. Sorghum Millet Newslett. 1997;38:29-30.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Frankel R., Scowcroft W.R., Whitfeld P.R. Chloroplast DNA variation in isonuclear male-sterile lines of Nicotiana. Mol. Gen. Genet. 1979;169:129-135.</mixed-citation><mixed-citation xml:lang="en">Frankel R., Scowcroft W.R., Whitfeld P.R. Chloroplast DNA variation in isonuclear male-sterile lines of Nicotiana. Mol. Gen. Genet. 1979;169:129-135.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Fujii S., Komatsu S., Toriyama K. Retrograde regulation of nuclear gene expression in CW-CMS of rice. Plant Mol. Biol. 2007;63:405-417. DOI: 10.1007/s11103-006-9097-8</mixed-citation><mixed-citation xml:lang="en">Fujii S., Komatsu S., Toriyama K. Retrograde regulation of nuclear gene expression in CW-CMS of rice. Plant Mol. Biol. 2007;63:405-417. DOI: 10.1007/s11103-006-9097-8</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Heng S., Wei C., Jing B., Wan Z., Wen J., Yi B., Ma C., Tu J., Fu T., Shen J. Comparative analysis of mitochondrial genomes between the hau cytoplasmic male sterility (CMS) line and its iso-nuclear maintainer line in Brassica juncea to reveal the origin of the CMSassociated gene orf288. BMC Genomics. 2014;15:322-334. DOI: 10.1186/1471-2164-15-322</mixed-citation><mixed-citation xml:lang="en">Heng S., Wei C., Jing B., Wan Z., Wen J., Yi B., Ma C., Tu J., Fu T., Shen J. Comparative analysis of mitochondrial genomes between the hau cytoplasmic male sterility (CMS) line and its iso-nuclear maintainer line in Brassica juncea to reveal the origin of the CMSassociated gene orf288. BMC Genomics. 2014;15:322-334. DOI: 10.1186/1471-2164-15-322</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Lopez-Juez E., Pyke K.A. Plastids unleashed: their development and their integration in plant development. Int. J. Dev. Biol. 2005;49(5/6): 557-577. DOI: 10.1387/ijdb.051997el</mixed-citation><mixed-citation xml:lang="en">Lopez-Juez E., Pyke K.A. Plastids unleashed: their development and their integration in plant development. Int. J. Dev. Biol. 2005;49(5/6): 557-577. DOI: 10.1387/ijdb.051997el</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Masuda T., Fujita Y. Regulation and evolution of chlorophyll metabolism. Photochem. Photobiol. Sci. 2008;7(10):131-1149. DOI: 10.1039/b807210h</mixed-citation><mixed-citation xml:lang="en">Masuda T., Fujita Y. Regulation and evolution of chlorophyll metabolism. Photochem. Photobiol. Sci. 2008;7(10):131-1149. DOI: 10.1039/b807210h</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Moran J.L., Rooney W.L. Effect of cytoplasm on the agronomic performance of grain Sorghum hybrids. Crop Sci. 2003;43:777-781. DOI: 10.2135/cropsci2003.0777</mixed-citation><mixed-citation xml:lang="en">Moran J.L., Rooney W.L. Effect of cytoplasm on the agronomic performance of grain Sorghum hybrids. Crop Sci. 2003;43:777-781. DOI: 10.2135/cropsci2003.0777</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Pogson B.J., Albrecht V. Genetic dissection of chloroplast biogenesis and development: an overview. Plant Physiol. 2011;155:1545-1551. DOI: 10.1104/pp.110.170365</mixed-citation><mixed-citation xml:lang="en">Pogson B.J., Albrecht V. Genetic dissection of chloroplast biogenesis and development: an overview. Plant Physiol. 2011;155:1545-1551. DOI: 10.1104/pp.110.170365</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Pring D.R., Tang H.V., Schertz K.F. Cytoplasmic male sterility and organelle DNAs of sorghum. Eds C.S. Levings, I.K. Vasil. The Molecular Biology of Plant Mitochondria. Dordrecht: Kluwer Acad. Publ., 1995.</mixed-citation><mixed-citation xml:lang="en">Pring D.R., Tang H.V., Schertz K.F. Cytoplasmic male sterility and organelle DNAs of sorghum. Eds C.S. Levings, I.K. Vasil. The Molecular Biology of Plant Mitochondria. Dordrecht: Kluwer Acad. Publ., 1995.</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Reddy B.V.S., Ramesh S., Ortiz R. Genetic and cytoplasmic-nuclear male sterility in Sorghum. Plant Breeding Reviews. Ed. J. Janik. Hoboken, New Jersy: Willey &amp; Sons, Inc. 2005;25:139-169. DOI: 10.1002/9780470650301.ch6</mixed-citation><mixed-citation xml:lang="en">Reddy B.V.S., Ramesh S., Ortiz R. Genetic and cytoplasmic-nuclear male sterility in Sorghum. Plant Breeding Reviews. Ed. J. Janik. Hoboken, New Jersy: Willey &amp; Sons, Inc. 2005;25:139-169. DOI: 10.1002/9780470650301.ch6</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Satyavathi C., Begum S., Singh B., Unnikrishnan K., Bharadwaj C. Analysis of diversity among cytoplasmic male sterile sources and their utilization in developing F1 hybrids in Pearl millet [Pennisetum glaucum (R.) Br]. Indian J. Genet. Plant Breed. 2009;69(4): 352-360.</mixed-citation><mixed-citation xml:lang="en">Satyavathi C., Begum S., Singh B., Unnikrishnan K., Bharadwaj C. Analysis of diversity among cytoplasmic male sterile sources and their utilization in developing F1 hybrids in Pearl millet [Pennisetum glaucum (R.) Br]. Indian J. Genet. Plant Breed. 2009;69(4): 352-360.</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Tanaka Y., Tsuda M., Yasumoto K., Yamagishi H., Terachi T. A complete of Ogura-Type male-starile cytoplasm andits comparative analysis with that of normal cytoplasm in radish (Raphanus sativus L.). BMC Genomics. 2012;13:352-363. DOI: 10.1186/1471-2164-13-352</mixed-citation><mixed-citation xml:lang="en">Tanaka Y., Tsuda M., Yasumoto K., Yamagishi H., Terachi T. A complete of Ogura-Type male-starile cytoplasm andits comparative analysis with that of normal cytoplasm in radish (Raphanus sativus L.). BMC Genomics. 2012;13:352-363. DOI: 10.1186/1471-2164-13-352</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Tang S., Sun Y., Zang H., Gu Y., Lu J., Tian S., Yu B., Gu M. Comparison on the characteristics of the isonuclear alloplasmic CMS lines in japonica Rice. Chines J. Rice Sci. 2005;19(6):521-526.</mixed-citation><mixed-citation xml:lang="en">Tang S., Sun Y., Zang H., Gu Y., Lu J., Tian S., Yu B., Gu M. Comparison on the characteristics of the isonuclear alloplasmic CMS lines in japonica Rice. Chines J. Rice Sci. 2005;19(6):521-526.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
