vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-11vavilov-4480Research ArticleГЕНЕТИКА ЖИВОТНЫХANIMAL GENETICSОсь «печень-мозг» при хронической инфекции Opisthorchis felineus в сочетании с длительной алкоголизацией мышейThe liver-brain axis under the influence of chronic Opisthorchis felineus infection combined with prolonged alcoholizationhttps://orcid.org/0000-0003-2462-3520АвгустиновичД. Ф.AvgustinovichD. F.

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Novosibirsk

avgust@bionet.nsc.ruhttps://orcid.org/0000-0002-2724-5441ЧадаеваИ. В.ChadaevaI. V.

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Novosibirsk

https://orcid.org/0000-0001-5192-0112КизименкоА. В.KizimenkoA. V.

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Novosibirsk

https://orcid.org/0000-0001-9543-9005КовнерА. В.KovnerA. V.

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Novosibirsk

https://orcid.org/0000-0001-5786-7965БазовкинаД. В.BazovkinaD. V.

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Novosibirsk

https://orcid.org/0000-0002-6493-0501ПономарёвД. В.PonomarevD. V.

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Novosibirsk

https://orcid.org/0000-0002-0686-3099ЕвсеенкоВ. И.EvseenkoV. I.

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Novosibirsk

НапримеровВ. А.NaprimerovV. A.

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Novosibirsk

https://orcid.org/0000-0003-1302-3626ЛьвоваМ. Н.LvovaM. N.

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Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Институт химии твердого тела и механохимии Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Institute of Solid State Chemistry and Mechanochemistry of the Siberian Branch of the Russian Academy of SciencesRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationИнститут химии твердого тела и механохимии Сибирского отделения Российской академии наукРоссияInstitute of Solid State Chemistry and Mechanochemistry of the Siberian Branch of the Russian Academy of SciencesRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский государственный аграрный университетРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State Agrarian UniversityRussian Federation20250303202529192107Copyright © Августинович Д.Ф., Чадаева И.В., Кизименко А.В., Ковнер А.В., Базовкина Д.В., Пономарёв Д.В., Евсеенко В.И., Напримеров В.А., Львова М.Н., 20252025Августинович Д.Ф., Чадаева И.В., Кизименко А.В., Ковнер А.В., Базовкина Д.В., Пономарёв Д.В., Евсеенко В.И., Напримеров В.А., Львова М.Н.Avgustinovich D.F., Chadaeva I.V., Kizimenko A.V., Kovner A.V., Bazovkina D.V., Ponomarev D.V., Evseenko V.I., Naprimerov V.A., Lvova M.N.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4480

   Описторхоз – паразитарное заболевание, вызываемое трематодами семейства Opisthorchiidae, к которому относится вид Opisthorchis felineus, максимально представленный у жителей Обь-Иртышского речного бассейна Западной Сибири. Хроническое употребление алкоголя влечет за собой неизлечимые заболевания печени, которые могут быть усугублены привнесением гельминтной инфекции. Хроническое воспаление на фоне двух воздействий может вызывать нарушения в других органах и системах, в том числе в центральной нервной системе. Сочетание длительного потребления 20 % этанола и инфекции O. felineus моделировали у мышей C57BL/6 с целью исследования изменений в мозге таких животных. Были сформированы четыре группы мышей: OF – мыши, инфицированные O. felineus в течение 6 мес; Eth – мыши, потребляющие 20 % этанол; Eth+OF – мыши с сочетанным действием двух факторов; CON – контрольные мыши, не испытывающие эти воздействия. Оба фактора вызывали у мышей, особенно в группе Eth+OF, выраженные перидуктальный фиброз и холангиофиброз, пролиферацию желчных протоков и увеличение участков воспалительной инфильтрации в паренхиме печени. Одновременно с нарушениями в печени, во фронтальной коре мозга инфекционный фактор обусловливал усиление перицеллюлярного отека (мыши OF), сдерживаемого введением этанола (мыши Eth+OF). Также обнаружены колебания уровней белка (Iba1, IL-1β, IL-6, TNF) и экспрессии генов Aif1, Il1b, Il6 и Tnf в гиппокампе и особенно во фронтальной коре, предполагающие регион-специфическое нейровоспаление. Поведенческое тестирование мышей показало, что потребление этанола влияло на поведение мышей Eth and Eth+OF в тесте принудительного плавания и на Startle-рефлекс. В тесте открытого поля более выраженные изменения происходили у мышей OF. У животных всех трех экспериментальных групп, но особенно у OF, выявлена аномалия обоняния (на свежие листья мяты перечной). Полученные данные могут отражать нарушение регуляторных механизмов в головном мозге вследствие системного воспаления при сочетанном действии длительного употребления алкоголя и гельминтной инфекции.

   Our purpose was to model a combination of a prolonged consumption of ethanol with Opisthorchis felineus infection in mice. Four groups of C57BL/6 mice were compiled: OF, mice infected with O. felineus for 6 months; Eth, mice consuming 20 % ethanol; Eth+OF, mice subjected to both adverse factors; and CON, control mice not exposed to these factors. In the experimental mice, especially in Eth+OF, each treatment caused well-pronounced periductal and cholangio fibrosis, proliferation of bile ducts, and enlargement of areas of inflammatory infiltration in the liver parenchyma. Simultaneously with liver disintegration, the infectious factor caused – in the frontal cerebral cortex – the growth of pericellular edema (OF mice), which was attenuated by the administration of ethanol (Eth+OF mice). Changes in the levels of some proteins (Iba1, IL-1β, IL-6, and TNF) and in mRNA expression of genes Aif1, Il1b, Il6, and Tnf were found in the hippocampus and especially in the frontal cortex, implying region-specific neuroinflammation. Behavioral testing of mice showed that ethanol consumption influenced the behavior of Eth and Eth+OF mice in the forced swimming test and their startle reflex. In the open field test, more pronounced changes were observed in OF mice. In mice of all three experimental groups, especially in OF mice, a disturbance in the sense of smell was detected (fresh peppermint leaves). The results may reflect an abnormality of regulatory mechanisms of the central nervous system as a consequence of systemic inflammation under the combined action of prolonged alcohol consumption and helminth infection.

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The authors declare that there are no conflicts of interest present.