vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-12vavilov-4481Research ArticleГЕНЕТИКА ЖИВОТНЫХANIMAL GENETICSНаименьшее число хромосом в семействе Pteromalidae (Hymenoptera: Chalcidoidea): кариотип и другие генетические особенности Pachycrepoideus vindemmiaeThe lowest chromosome number in the family Pteromalidae (Hymenoptera: Chalcidoidea): the karyotype and other genetic features of Pachycrepoideus vindemmiae (Rondani, 1875)https://orcid.org/0000-0001-9909-7559ГохманВ. Е.GokhmanV. E.

Москва

Moscow

vegokhman@hotmail.comhttps://orcid.org/0000-0002-3464-3325РябининА. С.RyabininA. S.

Новосибирск

Novosibirsk

https://orcid.org/0000-0001-7405-1773БыковР. А.BykovR. A.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-2691-3241ИлинскийЮ. Ю.IlinskyYu. Yu.

Новосибирск; Калининград

Novosibirsk; Kaliningrad

Русское энтомологическое обществоРоссияRussian Entomological SocietyRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Центр иммунологии и клеточной биологии, Балтийский федеральный университет им. Иммануила КантаРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Center for Immunology and Cell Biology, Immanuel Kant Baltic Federal UniversityRussian Federation202503032025291108112Copyright © Гохман В.Е., Рябинин А.С., Быков Р.А., Илинский Ю.Ю., 20252025Гохман В.Е., Рябинин А.С., Быков Р.А., Илинский Ю.Ю.Gokhman V.E., Ryabinin A.S., Bykov R.A., Ilinsky Y.Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4481

   Изучены различные генетические особенности культуры hitman широко распространенного паразитоида Drosophilidae (Diptera) Pachycrepoideus vindemmiae (Rondani, 1875) (Pteromalidae, Pachyneurinae), созданной и поддерживаемой в Институте цитологии и генетики Сибирского отделения Российской академии наук (Новосибирск, Россия). Анализ высушенных на воздухе хромосомных препаратов, полученных из предкуколок, показывает, что число хромосом для самцов и самок этого наездника составляет n = 4 и 2n = 8 соответственно; это наименьшее из известных для семейства Pteromalidae. Все хромосомы в кариотипе данного вида являются метацентриками. Первая и вторая хромосомы близки по размерам, остальные существенно короче. Такие же результаты получены еще для одной культуры этого вида, содержавшейся в Московском государственном университете (Россия). Сравнение последовательности ДНК баркодинг-участка, т. е. митохондриального гена цитохром c-оксидазы (COI) культуры hitman P. vindemmiae, с информацией, доступной в базах данных GenBank и BoLD, показало, что эта культура кластеризуется с конспецифичными особями, происходящими из Китая, Турции и Италии. Хотя некоторые эндосимбионты ранее указывались как для рода Pachycrepoideus Ashmead, 1904, так и для самого P. vindemmiae, оказалось, что культура hitman свободна от эндосимбиотических бактерий, принадлежащих к родам Arsenophonus Gherna et al., 1991, Cardinium Zchori-Fein et al., 2004, Rickettsia da Rocha-Lima, 1916, Spiroplasma Saglio et al., 1973 и Wolbachia Hertig, 1936. Вышеприведенные данные пополняют наши знания о различных генетических особенностях наездников семейства Pteromalidae, и в частности P. vindemmiae.

   Various genetic features of the hitman strain of the widespread parasitoid of Drosophilidae (Diptera), Pachycrepoideus vindemmiae (Rondani, 1875) (Pteromalidae, Pachyneurinae) were studied. This strain was established and is maintained at the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences (Novosibirsk, Russia). An analysis of air-dried chromosome preparations from prepupae of this parasitoid showed that it has n = 4 and 2n = 8 in males and females, respectively, which is the lowest known chromosome number in the family Pteromalidae. All chromosomes in the karyotype of this species are metacentric. The first and second chromosomes are of similar size, the remaining ones are substantially shorter. The same results were obtained for an additional strain of this species kept at the Moscow State University (Moscow, Russia). A comparison of the DNA sequence of the barcoding region of the mitochondrial cytochrome c oxidase (COI) gene of the hitman strain of P. vindemmiae with those available from the GenBank and BoLD databases demonstrated that this strain clustered together with conspecifics originating from China, Turkey and Italy. Despite certain endosymbionts being previously reported for the genus Pachycrepoideus Ashmead, 1904 as well as for P. vindemmiae itself, the hitman strain turned out to be free of endosymbiotic bacteria in the genera Arsenophonus Gherna et al., 1991, Cardinium Zchori-Fein et al., 2004, Rickettsia da Rocha-Lima, 1916, Spiroplasma Saglio et al., 1973 and Wolbachia Hertig, 1936. The above-mentioned results improve our knowledge of various genetic features of parasitoids of the family Pteromalidae and those of P. vindemmiae in particular.

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The authors declare that there are no conflicts of interest present.