vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-25vavilov-4540Research ArticleГЕНЕТИКА НАСЕКОМЫХINSECT GENETICSХарактеристика трех митохондриальных геномов комаров рода Aedes (Diptera: Culicidae) подрода StegomyiaMitochondrial genome variation of mosquito species in the subgenus Stegomyia of the genus Aedes (Diptera: Culicidae)БегаА. Г.BegaA. G.

Мытищи, Московская область; Москва

Mytishchi, Moscow Region; Moscow

ГорячеваИ. И.GoryachevaI. I.

Мытищи, Московская область; Москва

Mytishchi, Moscow Region; Moscow

МоскаевА. В.MoskaevA. V.

Мытищи, Московская область; Балашиха, Московская область

Mytishchi, Moscow Region; Balashikha, Moscow Region

АндриановБ. В.AndrianovB. V.

Москва

Moscow

andrianovb@mail.ruГосударственный университет просвещения; Институт общей генетики им. Н.И. Вавилова Российской академии наукРоссияFederal State University of Education; Vavilov Institute of General Genetics of the Russian Academy of SciencesRussian FederationГосударственный университет просвещения; Российский государственный университет народного хозяйства им. В.И. Вернадского Минсельхоза Российской ФедерацииРоссияFederal State University of Education; Vernadsky Russian State University of National EconomyRussian FederationИнститут общей генетики им. Н.И. Вавилова Российской академии наукРоссияVavilov Institute of General Genetics of the Russian Academy of Sciences,Russian Federation202510042025292219229Copyright © Бега А.Г., Горячева И.И., Москаев А.В., Андрианов Б.В., 20252025Бега А.Г., Горячева И.И., Москаев А.В., Андрианов Б.В.Bega A.G., Goryacheva I.I., Moskaev A.V., Andrianov B.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4540

Комары рода Aedes, подрода Stegomyia являются переносчиками ряда вирусов позвоночных, в том числе возбудителей арбовирусных лихорадок человека. Особый интерес представляет изучение генетических особенностей синантропных популяций видов этой группы. Мы получили, аннотировали и описали митохондриальные геномы трех видов комаров рода Aedes, подрода Stegomyia: Ae. albopictus, Ae. flavopictus и Ae. sibiricus. Митохондриальные геномы Ae. flavopictus и Ae. sibiricus были получены от комаров из синантропных популяций с Дальнего Востока России. Митохондриальный геном Ae. sibiricus представлен нами впервые. Митохондриальный геном Ae. albopictus был получен для клеточной линии C6/36. Мы подобрали три комплекта праймеров для каждого из видов комаров, которые амплифицируют весь митохондриальный геном, кроме контрольной области, и отсеквенировали геномы методом Сэнгера. Все три новых генома имеют одинаковый порядок расположения генов. Идентифицировано 13 канонических белок-кодирующих генов, 2 гена рибосомальной РНК, 22 гена транспортной РНК. Белок-кодирующие гены имеют канонические старт- и стоп-кодоны за двумя исключениями. Канонический стоп-кодон «TAA» неполный в генах cox1 и cox2. В гене cox1 отсутствует канонический старт-кодон для метионина. Нуклеотидная изменчивость представлена в основном точковыми нуклеотидными замещениями. Инсерции-делеции имеются в областях межгенных спейсеров. Проведен филогенетический анализ нуклеотидных последовательностей полных митохондриальных геномов всех известных видов комаров рода Aedes, подрода Stegomyia. Полученные данные позволили провести измерение соотношения синонимичных и несинонимичных замен (Ka/Ks) в конкретных белок-кодирующих генах.

Mosquitoes in the subgenus Stegomyia of the genus Aedes are vectors of a number of vertebrate viruses, including human arboviral fevers. Of particular interest is the study of the genetic characteristics of invasive populations of species in this group. We obtained, annotated and described the mitochondrial genomes of three Stegomyia mosquito species of the genus Aedes: Ae. albopictus, Ae. flavopictus and Ae. sibiricus. The mitochondrial genomes of Ae. flavopictus and Ae. sibiricus were obtained from mosquitoes from synanthropic populations in the Russian Far East. The mitochondrial genome of Ae. sibiricus is presented for the first time. The mitochondrial genome of Ae. albopictus was obtained for the C6/36 cell line. We selected three primer sets, for each mosquito species, that amplify the entire mitochondrial genome except for the control region and sequenced the genomes using the Sanger method. All three new genomes have an identical gene order. We identified 13 canonical protein-coding genes, 2 ribosomal RNA genes, and 22 transport RNA genes. Protein-coding genes have canonical start and stop codons with two exceptions. The canonical stop codon “TAA” is incomplete in the cox1 and cox2 genes. The cox1 gene lacks the canonical start codon for methionine. Nucleotide variability is mainly represented by point nucleotide substitutions. A phylogenetic analysis of the nucleotide sequences of complete mitochondrial genomes of all known mosquitoes species in the subgenus Stegomyia of the genus Aedes was performed. The data obtained made it possible to measure the ratio of synonymous to non-synonymous substitutions (Ka/Ks) in specific protein-coding genes.

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The authors declare that there are no conflicts of interest present.