vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-28vavilov-4543Research ArticleСИМБИОТИЧЕСКИЕ СИСТЕМЫSYMBIOTIC SYSTEMSТранскриптомный анализ признака симбиотической отзывчивости у гороха посевного (Pisum sativum L.)Transcriptomic analysis of the symbiotic responsivity trait in pea (Pisum sativum L.)КузьминаД. О.KuzminaD. O.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

ЗоринЕ. А.ZorinE. A.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

СулимаА. С.SulimaA. S.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

РоманюкД. А.RomanyukD. A.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

ГордонМ. Л.GordonM. L.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

ЖернаковА. И.ZhernakoA. I.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

КулаеваО. А.KulaevaO. A.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

АхтемоваГ. А.AkhtemovaG. A.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

ШтаркО. Ю,ShtarkO. .

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

ТихоновичИ. А.TikhonovichI. A.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

ЖуковВ. А.ZhukovV. A.

Пушкин-8, Санкт-Петербург

Pushkin 8, St. Petersburg

vzhukov@arriam.ruВсероссийский научно-исследовательский институт сельскохозяйственной микробиологииРоссияAll-Russia Research Institute for Agricultural MicrobiologRussian Federation202510042025292248258Copyright © Кузьмина Д.О., Зорин Е.А., Сулима А.С., Романюк Д.А., Гордон М.Л., Жернаков А.И., Кулаева О.А., Ахтемова Г.А., Штарк О.Ю., Тихонович И.А., Жуков В.А., 20252025Кузьмина Д.О., Зорин Е.А., Сулима А.С., Романюк Д.А., Гордон М.Л., Жернаков А.И., Кулаева О.А., Ахтемова Г.А., Штарк О.Ю., Тихонович И.А., Жуков В.А.Kuzmina D.O., Zorin E.A., Sulima A.S., Romanyuk D.A., Gordon M.L., Zhernako A.I., Kulaeva O.A., Akhtemova G.A., Shtark O..., Tikhonovich I.A., Zhukov V.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4543

Горох посевной (Pisum sativum L.) является важной сельскохозяйственной культурой и модельным объектом для изучения молекулярно-генетических основ азотфиксирующего симбиоза и арбускулярной микоризы (АМ). Описаны генотипы гороха с высокой и низкой отзывчивостью на инокуляцию клубеньковыми бактериями (ризобиями) и АМ грибами: «отзывчивые» генотипы демонстрируют прибавку массы семян при инокуляции, а для «неотзывчивых» генотипов такая реакция не характерна. С целью описания молекулярно-генетических механизмов, лежащих в основе симбиотической отзывчивости, был проведен транскриптомный анализ целых корневых систем растений гороха «отзывчивого» генотипа к-8274 (сорт Vendevil, Франция) и «неотзывчивого» генотипа к-3358 (сорт из Саратовской области, Россия), выращенных в почве без инокуляции (контроль) и при инокуляции ризобиями (одиночная инокуляция) и ризобиями совместно с АМ грибами (двойная инокуляция). «Отзывчивый» генотип действительно продемонстрировал выраженный ответ на уровне транскриптома на одиночную и двойную инокуляцию, в отличие от «неотзывчивого» генотипа. Одиночная инокуляция привела у к-8274 к специфическому повышению экспрессии генов, связанных с катаболизмом полиаминов, метаболизмом липидов и сигналингом на основе жасмоновой и салициловой кислот. При двойной инокуляции у к-8274 была повышена экспрессия генов, связанных с арбускулярно-микоризной инфекцией, и понижена экспрессия генов, связанных с клубенькообразованием. Данный факт соответствует фенотипу растений: число клубеньков у к-8274 было снижено при двойной инокуляции по сравнению с контролем. Таким образом, одним из механизмов, лежащих в основе симбиотической отзывчивости у гороха, может быть строгий контроль над числом клубеньков. Наконец, сравнение экспрессионных профилей корней растений к-8274 и к-3358 в условиях двойной инокуляции позволило идентифицировать «транскриптомные сигнатуры», характерные для симбиотически отзывчивого генотипа к-8274. Дальнейшая работа будет направлена на подтверждение возможности использования выявленных генов в качестве транскриптомных маркеров признака симбиотической отзывчивости у гороха посевного.

Pea (Pisum sativum L.) is an important crop culture and a model object for studying the molecular genetic bases of nitrogen-fixing symbiosis and arbuscular mycorrhiza (AM). Pea genotypes with high and low responsivity to inoculation with nodule bacteria (rhizobia) and AM fungi have been described: the ‘responsive’ genotypes demonstrate an increase in seed weight under inoculation, while ‘non-responsive’ ones do not show such a reaction. In order to get insight into the molecular genetic mechanisms underlying the symbiotic responsivity, a transcriptomic analysis of whole root systems of pea plants of the ‘responsive’ genotype k-8274 (cv. Vendevil, France) and ‘non-responsive’ genotype k-3358 (unnamed cultivar, Saratov region, Russia) grown in soil without inoculation (control) and inoculated either with rhizobia (single inoculation) or with rhizobia together with AM fungi (double inoculation) was performed. It was shown that the ‘responsive’ genotype, indeed, demonstrated a pronounced transcriptomic response to single and double inoculation, in contrast to the ‘non-responsive’ genotype. In k-8274, single inoculation led to specific up-regulation of genes related to catabolism of polyamines, lipid metabolism, and jasmonic acid and salicylic acid signaling. Under double inoculation, the specifically up-regulated genes in k-8274 were related to arbuscular mycorrhiza infection, and the down-regulated genes were related to nodulation. This fact matches the phenotype of the plants: the number of nodules was lower in k-8274 under double inoculation as compared to the control. Thus, strict control over the nodule number may be one of the mechanisms underlying the symbiotic responsivity of pea. Finally, a comparison of expression profiles in k-8274 and k-3358 roots under double inoculation also allowed us to identify the transcriptomic signatures characteristic of the symbiotically responsive genotype. Further work will be focused on validation of these transcriptomic markers of the symbiotic responsivity trait in pea.

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The authors declare that there are no conflicts of interest present.