<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vavilov-46</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>ОЦЕНКА ГЕНЕТИЧЕСКОГО ПОЛИМОРФИЗМА ОБРАЗЦОВ ЯРОВОЙ ТРИТИКАЛЕ (× TRITICOSECALE WITTMACK) ПОСРЕДСТВОМ RAPD- И ISSR-МАРКЕРОВ</article-title><trans-title-group xml:lang="en"><trans-title>EVALUATION OF GENETIC POLYMORPHISM OF SPRING TRITICALE ACCESSIONS (× TRITICOSECALE WITTMACK) BASED ON RAPD AND ISSR MARKERS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Орловская</surname><given-names>О. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Orlovskaya</surname><given-names>O. A.</given-names></name></name-alternatives><email xlink:type="simple">O.Orlovskaya@igc.bas-net.by</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Корень</surname><given-names>Л. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Koren</surname><given-names>L. V.</given-names></name></name-alternatives><email xlink:type="simple">O.Orlovskaya@igc.bas-net.by</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хотылева</surname><given-names>Л. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Khotyleva</surname><given-names>L. V.</given-names></name></name-alternatives><email xlink:type="simple">O.Orlovskaya@igc.bas-net.by</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Государственное научное учреждение Институт генетики и цитологии&#13;
Национальной академии наук Беларуси, Минск, Республика Беларусь<country>Беларусь</country></aff><aff xml:lang="en">Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Belarus<country>Belarus</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2012</year></pub-date><pub-date pub-type="epub"><day>11</day><month>12</month><year>2014</year></pub-date><volume>16</volume><issue>1</issue><fpage>279</fpage><lpage>284</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Орловская О.А., Корень Л.В., Хотылева Л.В., 2014</copyright-statement><copyright-year>2014</copyright-year><copyright-holder xml:lang="ru">Орловская О.А., Корень Л.В., Хотылева Л.В.</copyright-holder><copyright-holder xml:lang="en">Orlovskaya O.A., Koren L.V., Khotyleva L.V.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/46">https://vavilov.elpub.ru/jour/article/view/46</self-uri><abstract><p>Проведено изучение молекулярно-генетической гетерогенности 20 образцов яровой тритикале. Использованные ISSR- и RAPD-маркеры выявили высокий уровень полиморфизма (в среднем 80,2 и 89,9 % соответственно), что дало возможность сгруппировать изученные образцы тритикале по степени генетического родства и подобрать генетически дивергентные родительские пары для скрещиваний с целью получения гибридов с эффектом гетерозиса.</p></abstract><trans-abstract xml:lang="en"><p>Molecular heterogeneity was studied in 20 spring triticale accessions by using ISSR and RAPD markers. The study revealed a high level of polymorphism (80,2 % and 89,9 %, respectively, on the average), which allowed the studied triticale accessions to be grouped according to the degree of genetic relationship and to choose genetically distant parental pairs for crosses to obtain heterotic hybrids.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>тритикале</kwd><kwd>RAPD- и ISSR-анализ</kwd><kwd>гетерозис</kwd></kwd-group><kwd-group xml:lang="en"><kwd>triticale</kwd><kwd>RAPD and ISSR markers</kwd><kwd>heterosis</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Генетические основы селекции растений: В 4 т. Т. 2. Частная генетика растений / Ред. А.В. Кильчевский, Л.В. Хотылева. Минск: Беларус. навука, 2010. 579 с.</mixed-citation><mixed-citation xml:lang="en">Генетические основы селекции растений: В 4 т. Т. 2. Частная генетика растений / Ред. А.В. Кильчевский, Л.В. Хотылева. Минск: Беларус. навука, 2010. 579 с.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Bernardo R. Prediction of maize single-cross performance using RFLPs and information from related hybrids // Crop Sci. 1994. V. 34. Nо 1. P. 20–25.</mixed-citation><mixed-citation xml:lang="en">Bernardo R. Prediction of maize single-cross performance using RFLPs and information from related hybrids // Crop Sci. 1994. V. 34. Nо 1. P. 20–25.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Diers B.W., Mcvetty P.B.E., Osborn T.C. Relationship between heterosis and genetic distance based on RFLP markers in oilseed rape (Brassica napus L.) // Crop Sci. 1996. V. 36. Nо 1. P. 79–83.</mixed-citation><mixed-citation xml:lang="en">Diers B.W., Mcvetty P.B.E., Osborn T.C. Relationship between heterosis and genetic distance based on RFLP markers in oilseed rape (Brassica napus L.) // Crop Sci. 1996. V. 36. Nо 1. P. 79–83.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Fabrizius M.A., Busch R.H., Khan K., Huckle L. Genetic diversity and heterosis of spring wheat crosses // Crop Sci. 1998. V. 38. Nо 4. P. 1108–1112.</mixed-citation><mixed-citation xml:lang="en">Fabrizius M.A., Busch R.H., Khan K., Huckle L. Genetic diversity and heterosis of spring wheat crosses // Crop Sci. 1998. V. 38. Nо 4. P. 1108–1112.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Fernandez M.E., Figueiras A.M., Benito C. The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identifi cation and genetic diversity among barley cultivars with known origin // Theor. Appl. Genet. 2002. V. 104. P. 845–851.</mixed-citation><mixed-citation xml:lang="en">Fernandez M.E., Figueiras A.M., Benito C. The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identifi cation and genetic diversity among barley cultivars with known origin // Theor. Appl. Genet. 2002. V. 104. P. 845–851.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Fischer S., Maurer H.P., Würschum T. et al. Development of heterotic groups in Triticale // Crop Sci. 2010. V. 50. Nо 2. P. 584–590.</mixed-citation><mixed-citation xml:lang="en">Fischer S., Maurer H.P., Würschum T. et al. Development of heterotic groups in Triticale // Crop Sci. 2010. V. 50. Nо 2. P. 584–590.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Goral H., Tyrka M., Spiss L. Assessing genetic variation to predict the breeding value of winter triticale cultivars and lines // J. Appl. Genet. 2005. V. 46. P. 125–131.</mixed-citation><mixed-citation xml:lang="en">Goral H., Tyrka M., Spiss L. Assessing genetic variation to predict the breeding value of winter triticale cultivars and lines // J. Appl. Genet. 2005. V. 46. P. 125–131.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Kantety R.V., Zeng X.P., Bennetzen J.L., Zehr B.E. Assessment of genetic diversity in dent and popcorn (Zea mays L.) inbred lines using inter simple sequence repeat (ISSR) amplifi cation // Mol. Breeding. 1995. V. 1. P. 365–373.</mixed-citation><mixed-citation xml:lang="en">Kantety R.V., Zeng X.P., Bennetzen J.L., Zehr B.E. Assessment of genetic diversity in dent and popcorn (Zea mays L.) inbred lines using inter simple sequence repeat (ISSR) amplifi cation // Mol. Breeding. 1995. V. 1. P. 365–373.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Kuleung C., Baenziger P.S., Kachman S.D., Dweikat I. Evaluating the genetic diversity of triticale with wheat and rye SSR markers // Crop Sci. 2006. V. 46. P. 1692–1700.</mixed-citation><mixed-citation xml:lang="en">Kuleung C., Baenziger P.S., Kachman S.D., Dweikat I. Evaluating the genetic diversity of triticale with wheat and rye SSR markers // Crop Sci. 2006. V. 46. P. 1692–1700.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Nagaoka T., Ogihara Y. Applicability of inter-simple sequence repeat polymorphysms in wheat for use as DNA markers in comparison to RFLP and RAPD markers // Theor. Appl. Genet. 1997. V. 94. P. 597–602.</mixed-citation><mixed-citation xml:lang="en">Nagaoka T., Ogihara Y. Applicability of inter-simple sequence repeat polymorphysms in wheat for use as DNA markers in comparison to RFLP and RAPD markers // Theor. Appl. Genet. 1997. V. 94. P. 597–602.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Nei M., Li W.-H. Mathematical model for studying genetic variation in terms of restriction endonucleases // Proc. Natl Acad. Sci. USA. 1979. V. 76. Nо 10. P. 5269–5273.</mixed-citation><mixed-citation xml:lang="en">Nei M., Li W.-H. Mathematical model for studying genetic variation in terms of restriction endonucleases // Proc. Natl Acad. Sci. USA. 1979. V. 76. Nо 10. P. 5269–5273.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Nimbal S., Behl R.K., Chhabra A.K. RAPD analysis for genetic polymorphism in bread wheat (Triticum aestivum L.) genotypes varying for grain protein content // The South Pacifi c J. Nat. Sci. 2009. V. 27. P. 49–56.</mixed-citation><mixed-citation xml:lang="en">Nimbal S., Behl R.K., Chhabra A.K. RAPD analysis for genetic polymorphism in bread wheat (Triticum aestivum L.) genotypes varying for grain protein content // The South Pacifi c J. Nat. Sci. 2009. V. 27. P. 49–56.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Oettler G., Tams S.H., Utz H.F. et al. Prospects for hybrid breeding in winter triticale: Heterosis and combining ability for agronomic traits in European elite germplasm // Crop Sci. 2005. V. 45. Nо 4. P. 1476–1482.</mixed-citation><mixed-citation xml:lang="en">Oettler G., Tams S.H., Utz H.F. et al. Prospects for hybrid breeding in winter triticale: Heterosis and combining ability for agronomic traits in European elite germplasm // Crop Sci. 2005. V. 45. Nо 4. P. 1476–1482.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Salem H.H., Ali B.A., Huang T.-H. et al. Use of random amplifi ed polymorphic DNA analysis for economically important food crops // J. Integr. Plant Biol. 2007. V. 49. Nо 12. P. 1670–1680.</mixed-citation><mixed-citation xml:lang="en">Salem H.H., Ali B.A., Huang T.-H. et al. Use of random amplifi ed polymorphic DNA analysis for economically important food crops // J. Integr. Plant Biol. 2007. V. 49. Nо 12. P. 1670–1680.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Sofalian O., Chaparzadeh N., Javanmard A., Hejazi M.S. Study the genetic diversity of wheat landraces from northwest of Iran based on ISSR molecular markers // Int. J. Agriс. Biol. 2008. V. 10. Nо 3. P. 466–468.</mixed-citation><mixed-citation xml:lang="en">Sofalian O., Chaparzadeh N., Javanmard A., Hejazi M.S. Study the genetic diversity of wheat landraces from northwest of Iran based on ISSR molecular markers // Int. J. Agriс. Biol. 2008. V. 10. Nо 3. P. 466–468.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Sozen E. Evaluation of ISSR markers to assess genetic variability and relationship among winter triticale (× Triticosecale Wittmack) cultivars // Pak. J. Bot. 2010. V. 42. Nо 4. P. 2755–2763.</mixed-citation><mixed-citation xml:lang="en">Sozen E. Evaluation of ISSR markers to assess genetic variability and relationship among winter triticale (× Triticosecale Wittmack) cultivars // Pak. J. Bot. 2010. V. 42. Nо 4. P. 2755–2763.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Tams S.H., Melchinger A.E., Bauer E. Genetic similarity among European winter triticale elite germplasms assessed with AFLP and comparisons with SSR and pedigree data // Plant Breed. 2005. V. 124. Nо 3. P. 154–160.</mixed-citation><mixed-citation xml:lang="en">Tams S.H., Melchinger A.E., Bauer E. Genetic similarity among European winter triticale elite germplasms assessed with AFLP and comparisons with SSR and pedigree data // Plant Breed. 2005. V. 124. Nо 3. P. 154–160.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Tams S.H., Bauer E., Oettler G. et al. Prospects for hybrid breeding in winter triticale: II. Relationship between parental genetic distance and specifi c combining ability // Plant Breed. 2006. V. 125. Nо 5. P. 331–336.</mixed-citation><mixed-citation xml:lang="en">Tams S.H., Bauer E., Oettler G. et al. Prospects for hybrid breeding in winter triticale: II. Relationship between parental genetic distance and specifi c combining ability // Plant Breed. 2006. V. 125. Nо 5. P. 331–336.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers // Nucl. Acids Res. 1990. V. 18. P. 7213–7218.</mixed-citation><mixed-citation xml:lang="en">Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers // Nucl. Acids Res. 1990. V. 18. P. 7213–7218.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Van De Peer Y., De Watcher R. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment // Comput. Applic. Biosci. 1994. V. 10. P. 569–570.</mixed-citation><mixed-citation xml:lang="en">Van De Peer Y., De Watcher R. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment // Comput. Applic. Biosci. 1994. V. 10. P. 569–570.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
