vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-48vavilov-4611Research ArticleМЕДИЦИНСКАЯ ГЕНЕТИКАMEDICAL GENETICSАнализ экспрессии микроРНК и днкРНК в висцеральной жировой ткани у лиц с ожирением и без ожиренияExpression analysis of microRNA and lncRNA in visceral adipose tissue of obese and non-obese individualsБейркдарА.BairqdarA.

Новосибирск

Novosibirsk

ИванощукД. Е.IvanoshchukD. E.

Новосибирск

Novosibirsk

ТузовскаяО. В.TuzovskayaO. V.

Новосибирск

Novosibirsk

ШироковаН. С.ShirokovaN. S.

Новосибирск

Novosibirsk

КаштановаЕ. В.KashtanovaE.

Новосибирск

Novosibirsk

ПолонскаяЯ. В.PolonskayaY. V.

Новосибирск

Novosibirsk

РагиноЮ. И.RaginoY. I.

Новосибирск

Novosibirsk

ШахтшнейдерЕ. В.ShakhtshneiderE. V.

Новосибирск

Novosibirsk

shakhtshneyderev@bionet.nsc.ruФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университетРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Department of Genetics, Novosibirsk State UniversityRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Научно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Research Institute of Internal and Preventive Medicine – Branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationНаучно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияResearch Institute of Internal and Preventive Medicine – Branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian Federation202503062025293448457Copyright © Бейркдар А., Иванощук Д.Е., Тузовская О.В., Широкова Н.С., Каштанова Е.В., Полонская Я.В., Рагино Ю.И., Шахтшнейдер Е.В., 20252025Бейркдар А., Иванощук Д.Е., Тузовская О.В., Широкова Н.С., Каштанова Е.В., Полонская Я.В., Рагино Ю.И., Шахтшнейдер Е.В.Bairqdar A., Ivanoshchuk D.E., Tuzovskaya O.V., Shirokova N.S., Kashtanova E., Polonskaya Y.V., Ragino Y.I., Shakhtshneider E.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4611

Длинные некодирующие РНК (lncRNA) и микроРНК (miRNA) играют важную роль во всех биологических процессах, включая адипогенез, липидный обмен и инсулиновый ответ. Анализ экспрессии lncRNA и miRNA в висцеральной жировой ткани человека может способствовать более глубокому пониманию их роли в развитии метаболических нарушений. Исследование направлено на изучение уровней экспрессии lncRNA (ASMER1, SNHG9, P5549, P19461 и GAS5) и miRNA (miR-26A, miR-222, miR-221 и miR-155) в висцеральной жировой ткани у людей с абдоминальным ожирением (n = 70) по сравнению с уровнями их экспрессии у лиц без абдоминального ожирения (n = 31) методом количественной ПЦР в реальном времени. Среди протестированных miRNA уровень экспрессии miR-26A был снижен в висцеральной жировой ткани у людей с ожирением. Среди изученных lncRNA GAS5 показал значительное повышение уровня экспрессии у пациентов с ожирением и сахарным диабетом 2-го типа (СД2) (n = 10) по сравнению с лицами с ожирением без СД2 (n = 60). Уровни экспрессии GAS5 показали слабую отрицательную корреляцию (p < 0.05, rs = 0.25) с уровнями miR-155 только у пациентов с ожирением. Положительная корреляция (p < 0.01, rs = 0.92) между SNHG9 и GAS5 была обнаружена в группе людей без ожирения, с уменьшением коэффициента корреляции у пациентов с ожирением (p < 0.01, rs = 0.67). Кроме того, уровни miR- 26A и miR-155 умеренно коррелировали в группе без ожирения (p < 0.05, rs = 0.47) и демонстрировали слабую корреляцию у пациентов с ожирением (p < 0.05, rs = 0.26). Наши результаты показали, что у пациентов с абдоминальным ожирением наблюдаются повышенные уровни экспрессии miR- 26A в висцеральной жировой ткани и слабая корреляция между экспрессией GAS5 и SNHG9 по сравнению с лицами без абдоминального ожирения.

Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play important roles in all biological processes,  including adipogenesis, lipid metabolism, and insulin response. Analyzing expression patterns of lncRNAs and miRNAs  in human visceral fat tissue can enhance our understanding of their roles in metabolic disorders. Our research aims to investigate the expression of lncRNAs (ASMER1, SNHG9, P5549, P19461, and GAS5) and miRNAs (miR-26A, miR-222, miR-221, and miR-155) in visceral adipose tissues of individuals with abdominal obesity (n = 70) compared to their le vels in non-obese participants (n = 31), using Real-Time PCR. Among the tested miRNAs, only miR-26A was significantly downregulated in the visceral adipose tissue of obese individuals, with no significant change in the expression of miR- 26A in obese people with or without type 2 diabetes. Similarly, of the tested lncRNAs, only GAS5 showed significantly higher expression levels in obese patients with type 2 diabetes (T2D) (n = 10) compared to obese patients without T2D (n = 60). To test possible interactions between the analyzed non-coding RNAs, we used Spearman’s bivariate correlation test. GAS5 expression levels showed a weak negative correlation (p < 0.05, rs = 0.25) with miR-155 levels in obese patients only. Conversely, a strong positive correlation (p < 0.01, rs = 0.92) between SNHG9 and GAS5 was found in the non-obese group, with a weaker correlation in abdominally obese patients (p < 0.01, rs = 0.67); additionally, miR-26A and miR-155 levels were moderately correlated in the non-obese group (p < 0.05, rs = 0.47) and were found to correlate weakly in obese patients (p < 0.05, rs = 0.26). Our results showed that abdominally obese participants de monstrated higher expression levels of miR-26A in visceral adipose tissue and a significantly lower correlation  between GAS5 and SNHG9 expression when compared to non-obese subjects.

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The authors declare that there are no conflicts of interest present.