vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-58vavilov-4680Research ArticleИММУНИТЕТ И ПРОДУКТИВНОСТЬ РАСТЕНИЙРецепторподобные киназы с лейцин-богатыми повторами подсемейства III участвуют в распознавании Pectobacterium spp. растениями семейства SolanaceaeReceptor-like leucine-rich repeat kinases of subfamily III are involved in the recognition of Pectobacterium spp. by Solanaceae plantsШрубЕ. В.ShrubE. V.

Минск

Minsk

КолубакоА. В.KalubakaN. V.

Минск

Minsk

ВычикП. В.VychykP. V.

Минск

Minsk

БадалянО. А.BadalyanO. A.

Минск

Minsk

НиколайчикЕ. А.NikolaichikY. A.

Минск

Minsk

nikolaichik@bsu.byБелорусский государственный университетБеларусьBelarusian State UniversityBelarus202520072025294549558Copyright © Шруб Е.В., Колубако А.В., Вычик П.В., Бадалян О.А., Николайчик Е.А., 20252025Шруб Е.В., Колубако А.В., Вычик П.В., Бадалян О.А., Николайчик Е.А.Shrub E.V., Kalubaka N.V., Vychyk P.V., Badalyan O.A., Nikolaichik Y.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4680

Геномы растений семейства Пасленовые содержат 6олее 600 генов рецепторных протеинкиназ с лейцин-6огатыми повторами (LRR-RLK), многие из которых, вероятно, связаны с детекцией патогенов, но лишь некоторые 6ыли функционально охарактеризованы. Энтеро6актерии рода Pectobacterium – основные 6актериальные патогены многих сельскохозяйственных культур, в том числе картофеля и других растений семейства Пасленовые. Для актуальных патогенов из рода Pectobacterium специфические иммунные рецепторы растений не описаны. Однако у Malus × domestica охарактеризовано четыре LRR-RLK из подсемейства LRRIII (DIPM1-4), специфически взаимодействующих с эффекторным 6елком DspE и участвующих в распознавании родственного энтеро6актериального фитопатогена Erwinia amylovora. Поскольку ортолог DspE является основным эффектором и у Pectobacterium spp., мы выполнили филогенетический анализ RLK-LRRIII растений семейства Пасленовые совместно с 6олее полно охарактеризованными LRR-RLKIII у Arabidopsis thaliana и выделили девять кластеров родственных RLK. Кластеризация и анализ опу6ликованных данных позволили функционально охарактеризовать это семейство RLK и предложить наи6олее вероятных кандидатов для проверки взаимодействия с основным эффектором пекто6актерий DspE. Тестирование киназных доменов репрезентативных представителей разных кластеров в дрожжевой двухги6ридной системе выявило четыре RLK растений семейства Пасленовые, которые взаимодействуют с эффектором DspE из Pectobacterium versatile (Pve). Уровень экспрессии генов этих RLK и их ортологов у разных растений семейства варьировал, но в целом 6ыл очень низким. При этом о6наружена сильная DspE-зависимая супрессия генов RLK2 и RLK5 у инфицированных Pve растений картофеля, а инактивация их ортологов предотвращала развитие сверхчувствительной реакции в листьях растений, инфильтрованных суспензиями Pve. Данная ра6ота расширяет понимание разноо6разия RLK подсемейства LRR-RLKIII и их роли в иммунитете растений и может спосо6ствовать селекции устойчивых к 6актериозам сортов растений семейства Пасленовые.

The genomes of Solanaceae plants contain over 600 receptor-like protein kinase genes with leucine-rich repeats (LRR-RLK), many likely associated with pathogen detection, but very few functionally characterized. Pectobacterium spp. are the major bacterial pathogens of agricultural crops, particularly potatoes and other Solanaceae plants. For relevant potato pathogens from the genus Pectobacterium, specific immune receptors have not been described in Solanaceae. However, in Malus × domestica, four LRR-RLK from the LRRIII subfamily (DIPM1-4) have been characterized as receptors for the related pathogen Erwinia amylovora. DIPMs specifically interact with the effector protein DspE and are involved in E. amylovora recognition. Since the DspE ortholog is also the main effector in Pectobacterium spp., we performed a phylogenetic analysis of LRRIII subfamily receptors in the most relevant Solanaceae representatives together with a much better characterized LRR-RLKIII of Arabidopsis thaliana and identified nine clusters of related RLKs. Clustering followed by analysis of published data allowed us to functionally characterize this RLK family and suggest the most likely candidates for checking interactions with the main effector of pectobacteria, DspE. Testing the kinase domains of representative cluster members in a yeast two-hybrid system revealed four Solanaceae RLKs interacting with the DspE effector from Pectobacterium versatile. Virus-induced silencing of these RLK genes demonstrated their involvement in P. versatile recognition. The RLK6 gene from Solanum bulbocastanum, which is not an ortholog of the DIPM proteins in apple, seems to be the most promising potential resistance gene. This work expands our understanding of LRR-RLKIII subfamily RLKs and their role in plant immunity, providing a foundation for future development of disease-resistant Solanaceae varieties.

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The authors declare that there are no conflicts of interest present.