vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-60vavilov-4682Research ArticleПОПУЛЯЦИОННАЯ ГЕНЕТИКАPOPULATION GENETICSДанные митохондриальной ДНК позволяют выделить субпопуляции широкоареального вида журавлей красавки (Anthropoides virgo)Mitochondrial DNA data allow distinguishing the subpopulations in the widespread Demoiselle crane (Anthropoides virgo)МудрикЕ. А.MudrikE. A.

Mосква

Moscow

mudrik@vigg.ruИльяшенкоЕ. И.IlyashenkoE. I.

Mосква

Moscow

КазимировП. А.KazimirovP. A.

Mосква

Moscow

КондраковаК. Д.KondrakovaK. D.

Mосква

Moscow

АрчимаеваТ. П.ArchimaevaT. P.

Кызыл

Kyzyl

БазаровЛ. Д.BazarovL. D.

Кырен

Kyren

ГорошкоО. А.GoroshkoO. A.

Чита, Нижний Цасучей

Chita, Tsasuchey

ДоржиевЦ. З.DorzhievTs. Z.

Улан-Удэ

Ulan-Ude

КуксинА. Н.KuksinA. N.

Кызыл

Kyzyl

ПостельныхК. А.PostelnykhK. A.

Брыкин Бор

Brykin Bor

ШуркинаВ. В.ShurkinaV. V.

A6акан

Abakan

ИльяшенкоВ. Ю.IlyashenkoV. Yu.

Mосква

Moscow

ШатохинаА. В.ShatokhinaA. V.

Mосква

Moscow

ПолитовД. В.PolitovD V.

Mосква

Moscow

Институт о6щей генетики им. Н.И. Вавилова Российской академии наукРоссияVavilov Institute of General Genetics of the Russian Academy of SciencesRussian FederationИнститут о6щей генетики им. Н.И. Вавилова Российской академии наук;РоссияVavilov Institute of General Genetics of the Russian Academy of Sciences; Severtsov Institute of Ecology and Evolution of the Russian Academy of SciencesRussian FederationИнститут о6щей генетики им. Н.И. Вавилова Российской академии наук; Институт про6лем экологии и эволюции им. A.Н. Северцова Российской академии наукРоссияVavilov Institute of General Genetics of the Russian Academy of Sciences; Severtsov Institute of Ecology and Evolution of the Russian Academy of SciencesRussian FederationТувинский институт комплексного освоения природных ресурсов Си6ирского отделения Российской академии наукРоссияTuvinian Institute for Exploration of Natural Resources of the Siberian Branch of the Russian Academy of SciencesRussian FederationНациональный парк «Тункинский»РоссияTunkinsky National ParkRussian FederationИнститут природных ресурсов, экологии и криологии Си6ирского отделения Российской академии наук; Государственный природный 6иосферный заповедник «Даурский»РоссияInstitute of Nature Resources, Ecology and Cryology of the Siberian Branch of the Russian Academy of Sciences; Daursky State Nature Biosphere Reserve, Nizhny TsasucheyRussian FederationБурятский государственный университет им. Доржи Банзарова; Институт о6щей и экспериментальной 6иологии Си6ирского отделения Российской академии наукРоссияBuryat State University named after Dorji Banzarov; Institute of General and Experimental Biology of the Siberian Branch of the Russian Academy of SciencesRussian FederationОкский государственный природный 6иосферный заповедникРоссияOka State Nature Biosphere ReserveRussian FederationГосударственный природный заповедник «Хакасский»РоссияKhakassky State Nature ReserveRussian FederationИнститут про6лем экологии и эволюции им. A.Н. Северцова Российской академии наукРоссияSevertsov Institute of Ecology and Evolution of the Russian Academy of SciencesRussian Federation202520072025294568577Copyright © Мудрик Е.А., Ильяшенко Е.И., Казимиров П.А., Кондракова К.Д., Арчимаева Т.П., Базаров Л.Д., Горошко О.А., Доржиев Ц.З., Куксин А.Н., Постельных К.А., Шуркина В.В., Ильяшенко В.Ю., Шатохина А.В., Политов Д.В., 20252025Мудрик Е.А., Ильяшенко Е.И., Казимиров П.А., Кондракова К.Д., Арчимаева Т.П., Базаров Л.Д., Горошко О.А., Доржиев Ц.З., Куксин А.Н., Постельных К.А., Шуркина В.В., Ильяшенко В.Ю., Шатохина А.В., Политов Д.В.Mudrik E.A., Ilyashenko E.I., Kazimirov P.A., Kondrakova K.D., Archimaeva T.P., Bazarov L.D., Goroshko O.A., Dorzhiev T.Z., Kuksin A.N., Postelnykh K.A., Shurkina V.V., Ilyashenko V.Y., Shatokhina A.V., Politov D.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4682

Впервые изучен полиморфизм последовательностей фрагмента гена мтДНК цитохрома b и на его основе охарактеризована популяционно-генетическая структура журавля красавки (Anthropoides virgo Linnaeus, 1778) на 6ольшей части ареала в России. Для 157 осо6ей идентифицировано 18 гаплотипов, девять из которых оказались уникальными. Европейские вы6орки характеризовались 6ольшей гаплотипической и нуклеотидной изменчивостью и 6олее сильной генетической дифференциацией, чем азиатские. Поток генов между разными частями ареала красавки, вероятно, осуществляется через птиц, гнездящихся в 3ауралье. О6щая генетическая дифференциация вида составила >20 % (FST = 0.265, p < 0.001). Структура генофонда сформирована тремя основными гаплотипами, один из которых прео6ладает в Aзово-Черноморском регионе, второй – в Прикаспийском и Волго-Уральском, а третий наи6олее распространен в азиатских вы6орках. Исходя из соответствия внутривидовой генетической дифференциации красавки пролетным путям птиц из разных частей ареала, мы предлагаем в структуре вида выделить следующие су6популяции: 1) азово-черноморско-чадскую; 2) прикаспийско-суданскую; 3) зауральско-индийскую; 4) южноси6ирско-индийскую; 5) 6айкальско-индийскую; 6) за6айкальско-индийскую. Полученные данные создают основу для мониторинга генетического разноо6разия красавки и разра6отки научного о6основания мер охраны генофонда как вида в целом, так и его отдельных су6популяций.

The polymorphism of the mtDNA cytochrome b (cyt b) gene’s partial sequences has been studied in the Demoiselle crane (Anthropoides virgo Linnaeus, 1778) for the first time. Based on cyt b variability, the population genetic structure of the species was characterized within most of its range in Russia. Among 157 individuals we identified 18 haplotypes, nine of which were unique. In the European samples, we observed greater haplotype and nucleotide diversity and stronger genetic differentiation than in the Asian ones. Gene flow between different parts of the Demoiselle crane range is probably mediated by birds breeding in the Trans-Urals. The overall genetic subdivision of the species as estimated by FST was 0.265 (p < 0.001). The structure of the gene pool is formed by three main haplotypes, one of which predominates in the Azov-Black Sea region, the second in the Caspian and Volga-Ural regions, and the third is most common in the Asian samples. Based on the correspondence of intraspecific genetic differentiation of the Demoiselle cranes from different parts of the range to their flyways, we propose to distinguish the following subpopulations: (1) Azov-Black Sea/Chadian; (2) Caspian/Sudanese; (3) Trans-Ural/Indian; (4) South Siberian/Indian; (5) Baikal/Indian and (6) Trans-Baikal/Indian. The obtained data create the basis for monitoring the genetic diversity of the Demoiselle crane and developing a scientific background for measures to protect the gene pool of the species as a whole and its subpopulations.

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The authors declare that there are no conflicts of interest present.