vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-125vavilov-4918Research ArticleХРОМОСОМНАЯ И ГЕНЕТИЧЕСКАЯ ИНЖЕНЕРИЯCHROMOSOME AND GENE ENGINEERINGИзучение влияния интрогрсии хромосомы 2 Аt-субгенома хлопчатника вида Gossypium barbadense L. при беккроссировании исходными линиями вида G. hirsutum LStudy of the influence of introgression from chromosome 2 of the At subgenome of cotton Gossypium barbadense L. during backcrossing with the original lines of G. hirsutum Lhttps://orcid.org/0000-0003-1840-0240СанамьянМ. Ф.SanamyanM. F.

Ташкент

Tashkent

sanam_marina@rambler.ruhttps://orcid.org/0009-0003-9434-1411БобохужаевШ. У.BobokhujayevSh. U.

Ташкент

Чирчик, Ташкентская область

Tashkent

Chirchik, Tashkent region

https://orcid.org/0000-0003-3801-6537АбдукаримовШ. С.AbdukarimovSh. S.

Ташкент

Кибрайский район, Ташкентская область

 

Tashkent

Kibrai district, Tashkent region

https://orcid.org/0009-0006-4064-120XУраловЖ. С.UralovJ. S.

Ташкент

Tashkent

https://orcid.org/0009-0003-9379-4689РустамовА. Б.RustamovA. B.

Ташкент

Tashkent

Национальный университет Узбекистана им. М. УлугбекаУзбекистанNational University of Uzbekistan named after Mirzo UlugbekUzbekistanНациональный университет Узбекистана им. М. Улугбека; Чирчикский государственный педагогический университетУзбекистанNational University of Uzbekistan named after Mirzo Ulugbek; Chirchik State Pedagogical UniversityUzbekistanНациональный университет Узбекистана им. М. Улугбека; Центр геномики и биоинформатики Академии наук Республики УзбекистанУзбекистанNational University of Uzbekistan named after Mirzo Ulugbek; Center of Genomics and Bioinformatics of the Academy of Sciences of the Republic of UzbekistanUzbekistan20251101202629811841194Copyright © Санамьян М.Ф., Бобохужаев Ш.У., Абдукаримов Ш.С., Уралов Ж.С., Рустамов А.Б., 20262026Санамьян М.Ф., Бобохужаев Ш.У., Абдукаримов Ш.С., Уралов Ж.С., Рустамов А.Б.Sanamyan M.F., Bobokhujayev S.U., Abdukarimov S.S., Uralov J.S., Rustamov A.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4918

Создание хромосомно-замещенных линий, содержащих одну пару хромосом родственного вида, является одним из способов интрогрессии чужеродного генетического материала. Известно, что частота встречаемости замещений по разным хромосомам генома различается по причине избирательности процесса трансмиссии чужеродных хромосом через гаметы гибридов. Использование моносомных линий с идентифицированными унивалентными хромосомами и молекулярно-генетических SSR-маркеров на стадии проростков позволило осуществить быстрый скрининг идентичности чужеродной хромосомы у беккроссных гибридов, что значительно ускорило и облегчило процесс беккроссирования при создании новых хромосомно замещенных линий хлопчатника. В ходе изучения процесса передачи хромосомы 2 Аt-субгенома хлопчатника Gossypium barbadense L. при беккроссировании четырех исходных моносомных линий вида G. hirsutum L. моносомными беккроссными гибридами с замещением хромосомы 2 Аt-субгенома были выявлены: снижение скрещиваемости, завязываемости и всхожести гибридных семян; отличия в частоте и характере передачи хромосомы 2 At-субгенома хлопчатника G. barbadensе; регулярность поведения хромосом в мейозе и высокий мейотический индекс; значительное снижение фертильности пыльцы у беккроссных моносомных гибридов BC1F1; специфические морфобиологические особенности моносомных беккроссных растений в виде задержки развития вегетативных и генеративных органов, низкорослости и сниженной облиственности, а также слабой бутонизации и цветения в течение первого года вегетации. По-видимому, такие специфические изменения происходили вследствие реорганизации гибридного генома и интрогрессии чужеродного хроматина. Кроме того, была продемонстрирована эффективность использования молекулярно-генетических микросателлитных (SSR) маркеров для контролирования процессов беккроссирования и элиминации генетического материала донорной линии Pima 3-79 вида G. barbadense с целью отбора генотипов с чужеродным замещением хромосом.

The creation of chromosome substitution lines containing one pair of chromosomes from a related species is one method for introgression of alien genetic material. The frequency of substitutions in different chromosomes of the genome varies due to the selective transmission of alien chromosomes through the gametes of hybrids. The use of monosomic lines with identified univalent chromosomes and molecular genetic SSR markers at the seedling stage allowed rapid screening of the identity of the alien chromosome in backcross hybrids, significantly accelerating and facilitating the backcrossing process for the creation of new chromosome substitution cotton lines. As a result of studying the process of transmission of chromosome 2 of the At subgenome of the cotton plant G. barbadense L. during backcrossing of four original monosomic lines of G. hirsutum L. with monosomic backcross hybrids with substitution of chromosome 2 of the At subgenome, the following specific consequences of the introgression of this chromosome were revealed: decreased crossability, setting and germination of hybrid seeds; differences in the frequency and nature of transmission of chromosome 2 of the At subgenome of the cotton plant G. barbadensе; regularity of chromosome behavior in meiosis; a high meiotic index; a significant decrease in pollen fertility in backcross monosomic hybrids BC1F1; specific morphobiological characteristics of monosomic backcrossed plants, such as delayed development of vegetative and generative organs; dwarfism; reduced foliage; and poor budding and flowering during the first year of vegetation. All of these factors negatively impact the study and backcrossing of monosomic hybrids and significantly complicate and delay the creation of chromosome-substituted forms concerning chromosome 2 of the At subgenome of cotton, G. barbadense. These specific changes likely occurred as a result of hybrid genome reorganization and introgression of alien chromatin. Furthermore, the effectiveness of using molecular genetic microsatellite (SSR) markers to monitor backcrossing processes and eliminate genetic material from the Pima 3-79 donor line of G. barbadense for the selection of genotypes with alien chromosome substitutions has been demonstrated.

хлопчатникG. hirsutumG. barbadenseмоносомные линиихромосомно-замещенные гибридыбеккроссированиеSSR-маркерыcottonG. hirsutumG. barbadensemonosomic lineschromosome-substituted hybridsbackcrossingSSR markersThis study was financially supported by the Ministry of Higher Education, Science, and Innovation of the Republic of Uzbekistan as part of the F-OT-2021-155 projectThis study was financially supported by the Ministry of Higher Education, Science, and Innovation of the Republic of Uzbekistan as part of the F-OT-2021-155 projectReferencesAbdullaev A.A. Evolution and Taxonomy of Polyploid Species of Cot ton. Tashkent, 1974 (in Russian)Abdullaev A.A. Evolution and Taxonomy of Polyploid Species of Cot ton. Tashkent, 1974 (in Russian)Adonina I.G., Timonova E.M., Salina E.A. Introgressive hybridization of common wheat: results and prospects. Russ J Genet. 2021;57(4): 390407. doi 10.1134/S1022795421030029Adonina I.G., Timonova E.M., Salina E.A. Introgressive hybridization of common wheat: results and prospects. Russ J Genet. 2021;57(4): 390407. doi 10.1134/S1022795421030029Dedukh D., Krasikova A. Delete and survive: strategies of programmed genetic material elimination in eukaryotes. Biol Rev Camb Philos Soc. 2021;97(1):195-216. doi 10.1111/brv.12796Dedukh D., Krasikova A. Delete and survive: strategies of programmed genetic material elimination in eukaryotes. Biol Rev Camb Philos Soc. 2021;97(1):195-216. doi 10.1111/brv.12796Endrizzi J.E., Turcotte E.L., Kohel R.J. Genetics, cytology and evo lution of Gossypium. Adv Genet. 1985;23:271-375. doi 10.1016/S00652660(08)605155Endrizzi J.E., Turcotte E.L., Kohel R.J. Genetics, cytology and evo lution of Gossypium. Adv Genet. 1985;23:271-375. doi 10.1016/S00652660(08)605155Evtushenko E.V., Lipikhina Y.A., Stepochkin P.I., Verchinin A.V. Cy togenetic and molecular characteristics of rye genome in octoploid triticale (× Triticosecale Wittmack). Comp Cytogenet. 2019;13(4): 423434. doi 10.3897/compcytogen.v13i4.39576Evtushenko E.V., Lipikhina Y.A., Stepochkin P.I., Verchinin A.V. Cy togenetic and molecular characteristics of rye genome in octoploid triticale (× Triticosecale Wittmack). Comp Cytogenet. 2019;13(4): 423434. doi 10.3897/compcytogen.v13i4.39576Fang D.D., Thyssen G.N., Wang M., Jenkins J.N., McCarty J.C., Jones D.C. Genomic confirmation of Gossypium barbadense intro gression into G. hirsutum and a subsequent MAGIC population. Mol Genet Genomics. 2023;298(1):143-152. doi 10.1007/s00438022019743Fang D.D., Thyssen G.N., Wang M., Jenkins J.N., McCarty J.C., Jones D.C. Genomic confirmation of Gossypium barbadense intro gression into G. hirsutum and a subsequent MAGIC population. Mol Genet Genomics. 2023;298(1):143-152. doi 10.1007/s00438022019743Gutiérrez O.A., Stelly D.M., Saha S., Jenkins J.N., McCarty J.C., Ras ka D.A., Scheffler B.E. Integrative placement and orientation of non redundant SSR loci in cotton linkage groups by deficiency analysis. Mol Breeding. 2009;23:693-707. doi 10.1007/s110320099266yGutiérrez O.A., Stelly D.M., Saha S., Jenkins J.N., McCarty J.C., Ras ka D.A., Scheffler B.E. Integrative placement and orientation of non redundant SSR loci in cotton linkage groups by deficiency analysis. Mol Breeding. 2009;23:693-707. doi 10.1007/s110320099266yIshii T., Karimi-Ashtiyani R., Houben A. Haploidization via chromo some elimination: means and mechanisms. Annu Rev Plant Biol. 2016;67:421-438. doi 10.1146/annurevarplant043014114714Ishii T., Karimi-Ashtiyani R., Houben A. Haploidization via chromo some elimination: means and mechanisms. Annu Rev Plant Biol. 2016;67:421-438. doi 10.1146/annurevarplant043014114714Jenkins J.N., Wu J., McCarty J.C., Saha S., Gutiérrez O., Hayes R., Stelly D.M. Genetic effects of thirteen Gossypium barbadense L. chromosome substitution lines in top crosses with upland cotton cultivars: I. Yield and yield components. Crop Sci. 2006;46(3): 11691178. doi 10.2135/cropsci2005.080269Jenkins J.N., Wu J., McCarty J.C., Saha S., Gutiérrez O., Hayes R., Stelly D.M. Genetic effects of thirteen Gossypium barbadense L. chromosome substitution lines in top crosses with upland cotton cultivars: I. Yield and yield components. Crop Sci. 2006;46(3): 11691178. doi 10.2135/cropsci2005.080269Jenkins J.N., McCarty J.C., Wu J., Saha S., Gutiérrez О., Hayes R., Stelly D.M. Genetic effects of thirteen Gossypium barbadense L. chromosome substitution lines in top crosses with upland cotton cultivars: II. Fiber quality traits. Crop Sci. 2007;47(2):561-572. doi 10.2135/cropsci2006.06.0396Jenkins J.N., McCarty J.C., Wu J., Saha S., Gutiérrez О., Hayes R., Stelly D.M. Genetic effects of thirteen Gossypium barbadense L. chromosome substitution lines in top crosses with upland cotton cultivars: II. Fiber quality traits. Crop Sci. 2007;47(2):561-572. doi 10.2135/cropsci2006.06.0396Krasilova N.M., Adonina I.G., Silkova O.G., Shumny V.K. Transmis sion of rye chromosome 2R in backcrosses of wheat rye 2R (2D sub stitution lines to various common wheat varieties. Vavilovskii Zhur nal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2011;15(3):554-562 (in Russian)Krasilova N.M., Adonina I.G., Silkova O.G., Shumny V.K. Transmis sion of rye chromosome 2R in backcrosses of wheat rye 2R (2D sub stitution lines to various common wheat varieties. Vavilovskii Zhur nal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2011;15(3):554-562 (in Russian)Li H., Guo X., Wang Ch., Ji W. Spontaneous and divergent hexaploid triticales derived from common wheat × rye by complete elimination of Dgenome chromosomes. PLoS One. 2015;10(3):e0120421. doi 10.1371/journal.pone.0120421Li H., Guo X., Wang Ch., Ji W. Spontaneous and divergent hexaploid triticales derived from common wheat × rye by complete elimination of Dgenome chromosomes. PLoS One. 2015;10(3):e0120421. doi 10.1371/journal.pone.0120421Liu S., Saha S., Stelly D.M., Burr B., Cantrell R.G. Chromosomal assignment of microsatellite loci in cotton. Hered. 2000;91(4): 326332. doi 10.1093/jhered/91.4.326Liu S., Saha S., Stelly D.M., Burr B., Cantrell R.G. Chromosomal assignment of microsatellite loci in cotton. Hered. 2000;91(4): 326332. doi 10.1093/jhered/91.4.326Mauer F.M. Origin and Taxonomy of Cotton. Vol. 1. Cotton. Tashkent, 1954 (in Russian) McClintock B. The significance of responses of the genome to challenge. Science. 1984;226(4676):792-801. doi 10.1126/science.15739260Mauer F.M. Origin and Taxonomy of Cotton. Vol. 1. Cotton. Tashkent, 1954 (in Russian) McClintock B. The significance of responses of the genome to challenge. Science. 1984;226(4676):792-801. doi 10.1126/science.15739260Merker A., Forsstrom P.O. Isolation of mildew resistant wheat-rye translocations from a double substitution line. Euphytica. 2000;115: 167172. doi 10.1023/A:1004018500970Merker A., Forsstrom P.O. Isolation of mildew resistant wheat-rye translocations from a double substitution line. Euphytica. 2000;115: 167172. doi 10.1023/A:1004018500970Morris E.R., Sears E.R. Cytogenetics of Wheat and Related Forms. Wheat and its Improvement. Moscow: Kolos Publ., 1970 (in Russian)Morris E.R., Sears E.R. Cytogenetics of Wheat and Related Forms. Wheat and its Improvement. Moscow: Kolos Publ., 1970 (in Russian)Polgári D., Mihók E., Sági L. Composition and random elimination of paternal chromosomes in a large population of wheatbarley (Triti cum aestivum L. × Hordeum vulgare L.) hybrids. Plant Cell Rep. 2019;38:767-775. doi 10.1007/s00299019024051Polgári D., Mihók E., Sági L. Composition and random elimination of paternal chromosomes in a large population of wheatbarley (Triti cum aestivum L. × Hordeum vulgare L.) hybrids. Plant Cell Rep. 2019;38:767-775. doi 10.1007/s00299019024051Saha S., Wu J., Jenkins J.N., McCarty J.C., Jr., Gutiérrez O.A., Stel ly D.M., Percy R.G., Raska D.A. Effect of chromosome substitu tions from Gossypium barbadense L. 379 into G. hirsutum L. TM1 on agronomic and fiber traits. J Cotton Sci. 2004;8(3):162-169Saha S., Wu J., Jenkins J.N., McCarty J.C., Jr., Gutiérrez O.A., Stel ly D.M., Percy R.G., Raska D.A. Effect of chromosome substitu tions from Gossypium barbadense L. 379 into G. hirsutum L. TM1 on agronomic and fiber traits. J Cotton Sci. 2004;8(3):162-169Saha S., Raska D.A., Stelly D.M. Upland cotton (Gossypium hirsu tum L.) × Hawaiian cotton (G. tomentosum Nutt. ex Seem.) F1 hy brid hypoaneuploid chromosome substitution series. J Cotton Sci. 2006;10(4):263-272Saha S., Raska D.A., Stelly D.M. Upland cotton (Gossypium hirsu tum L.) × Hawaiian cotton (G. tomentosum Nutt. ex Seem.) F1 hy brid hypoaneuploid chromosome substitution series. J Cotton Sci. 2006;10(4):263-272Saha S., Wu J., Jenkins J.N., McCarty J.C., Hayes R., Stelly D.M. Genetic dissection of chromosome substitution lines of cotton to discover novel Gossypium barbadense L. alleles for improvement of agronomic traits. Theor Appl Genet. 2010;120(6):1193-1205. doi 10.1007/s0012200912473Saha S., Wu J., Jenkins J.N., McCarty J.C., Hayes R., Stelly D.M. Genetic dissection of chromosome substitution lines of cotton to discover novel Gossypium barbadense L. alleles for improvement of agronomic traits. Theor Appl Genet. 2010;120(6):1193-1205. doi 10.1007/s0012200912473Saha S., Raska D.A., Stelly D.M., Manchali Sh., Gutiérrez O.A. Hypoaneuploid chromosome substitution F1 hybrids of Gossypium hirsutum L. × G. mustelinum Miers ex Watt. J Cotton Sci. 2013; 17(2):102-114Saha S., Raska D.A., Stelly D.M., Manchali Sh., Gutiérrez O.A. Hypoaneuploid chromosome substitution F1 hybrids of Gossypium hirsutum L. × G. mustelinum Miers ex Watt. J Cotton Sci. 2013; 17(2):102-114Saha S., Stelly D.M., Makamov A.K., Ayubov M.S., Raska D., Gutiérrez O.A., Shivapriya M., Jenkins J.N., Dewayne D., Abdu rakhmonov I.Y. Molecular confirmation of Gossypium hirsutum chromosome substitution lines. Euphytica. 2015;205:459-473. doi 10.1007/s1068101514072Saha S., Stelly D.M., Makamov A.K., Ayubov M.S., Raska D., Gutiérrez O.A., Shivapriya M., Jenkins J.N., Dewayne D., Abdu rakhmonov I.Y. Molecular confirmation of Gossypium hirsutum chromosome substitution lines. Euphytica. 2015;205:459-473. doi 10.1007/s1068101514072Saha S., Bellaloui N., Jenkins J.N., McCarty J.C., Stelly D.M. Ef fect of chromosome substitutions from Gossypium barbadense L., G. tomentosum Nutt. ex Seem and G. mustelinum Watt into G. hirsutum L. on cotton seed protein and oil content. Euphytica. 2020;216:118. doi 10.1007/s10681020026444Saha S., Bellaloui N., Jenkins J.N., McCarty J.C., Stelly D.M. Ef fect of chromosome substitutions from Gossypium barbadense L., G. tomentosum Nutt. ex Seem and G. mustelinum Watt into G. hirsutum L. on cotton seed protein and oil content. Euphytica. 2020;216:118. doi 10.1007/s10681020026444Sanamyan M.F., Bobokhujaev Sh.U. Identification of univalent chro mosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2019;23(7): 836845. doi 10.18699/VJ19.557 (in Russian)Sanamyan M.F., Bobokhujaev Sh.U. Identification of univalent chro mosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2019;23(7): 836845. doi 10.18699/VJ19.557 (in Russian)Sanamyan М.F., Petlyakova J., Rakhmatullina E.M., Sharipova E. Cy togenetic collection of Uzbekistan. In: Abdurakhmonov I.Y. (Ed.) World Cotton Germplasm Resources. InTech, 2014;247-287. doi 10.5772/58589Sanamyan М.F., Petlyakova J., Rakhmatullina E.M., Sharipova E. Cy togenetic collection of Uzbekistan. In: Abdurakhmonov I.Y. (Ed.) World Cotton Germplasm Resources. InTech, 2014;247-287. doi 10.5772/58589Sanamyan M.F., Bobokhujaev Sh.U., Makamov A.X., Achilov S.G., Abdurakhmonov I.Y. The creation of new aneuploid lines of the cot ton (Gossypium hirsutum L.) with identification of chromosomes by translocation and SSR markers. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2016a;20(5): 643652. doi 10.18699/VJ16.186 (in Russian)Sanamyan M.F., Bobokhujaev Sh.U., Makamov A.X., Achilov S.G., Abdurakhmonov I.Y. The creation of new aneuploid lines of the cot ton (Gossypium hirsutum L.) with identification of chromosomes by translocation and SSR markers. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2016a;20(5): 643652. doi 10.18699/VJ16.186 (in Russian)Sanamyan M.F., Makamov A.K., Bobokhujaev Sh.U., Usmonov D.E., Buriev Z.T., Saha S., Stelly D.M. The utilization of translocation lines and microsatellite markers for the identification of unknown cotton monosomic lines. In: Abdurakhmonov I.Y. (Ed.) Cotton Re search. InTech, 2016b;167-183. doi 10.5772/64558Sanamyan M.F., Makamov A.K., Bobokhujaev Sh.U., Usmonov D.E., Buriev Z.T., Saha S., Stelly D.M. The utilization of translocation lines and microsatellite markers for the identification of unknown cotton monosomic lines. In: Abdurakhmonov I.Y. (Ed.) Cotton Re search. InTech, 2016b;167-183. doi 10.5772/64558Sanamyan M.F., Bobokhujaev Sh.U., Abdukarimov Sh.S., Maka mov Kh.A., Silkova O.G. Features of chromosome introgression from Gossypium barbadense L. into G. hirsutum L. during the de velopment of alien substitution lines. Plants. 2022;11(4):542. doi 10.3390/plants11040542Sanamyan M.F., Bobokhujaev Sh.U., Abdukarimov Sh.S., Maka mov Kh.A., Silkova O.G. Features of chromosome introgression from Gossypium barbadense L. into G. hirsutum L. during the de velopment of alien substitution lines. Plants. 2022;11(4):542. doi 10.3390/plants11040542Sanamyan M.F., Bobokhujaev Sh.U., Abdukarimov Sh.S., Sil kova O.G. Moleculargenetic and cytogenetic analyses of cotton chromosome introgression from Gossypium barbadense L. into the genome of G. hirsutum L. in BC2F1 hybrids. Vavilovskii Zhur nal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2023;27(8):958-970. doi 10.18699/VJGB-23-110Sanamyan M.F., Bobokhujaev Sh.U., Abdukarimov Sh.S., Sil kova O.G. Moleculargenetic and cytogenetic analyses of cotton chromosome introgression from Gossypium barbadense L. into the genome of G. hirsutum L. in BC2F1 hybrids. Vavilovskii Zhur nal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2023;27(8):958-970. doi 10.18699/VJGB-23-110Sanei M., Pickering R., Kumke K., Nasuda S., Houben A. Loss of centromeric histone H3 (CENH3) from centromeres precedes uni parental chromosome elimination in interspecific barley hybrids. Proc Natl Acad Sci USA. 2011;108(33):E498-E505. doi 10.1073/pnas.1103190108Sanei M., Pickering R., Kumke K., Nasuda S., Houben A. Loss of centromeric histone H3 (CENH3) from centromeres precedes uni parental chromosome elimination in interspecific barley hybrids. Proc Natl Acad Sci USA. 2011;108(33):E498-E505. doi 10.1073/pnas.1103190108Shchapova A.I., Kravtsova L.A. Cytogenetics of Wheat-Rye Hybrids. Novosibirsk, 1990 (in Russian) Silkova O.G., Leonova I.N., Krasilova N.M., Dubovets N.I. Preferen tial elimination of chromosome 5R of rye in the progeny of 5R5D dimonosomics. Russ J Genet. 2011;47(8):942-950. doi 10.1134/S1022795411080151Shchapova A.I., Kravtsova L.A. Cytogenetics of Wheat-Rye Hybrids. Novosibirsk, 1990 (in Russian) Silkova O.G., Leonova I.N., Krasilova N.M., Dubovets N.I. Preferen tial elimination of chromosome 5R of rye in the progeny of 5R5D dimonosomics. Russ J Genet. 2011;47(8):942-950. doi 10.1134/S1022795411080151Tang D., Feng S., Li S., Chen Yu., Zhou B. Ten alien chromosome additions of Gossypium hirsutum–Gossypium bickii developed by integrative uses of GISH and species-specific SSR markers. Mol Genet Genomics. 2018;293(4):1-11. doi 10.1007/s00438018 14345Tang D., Feng S., Li S., Chen Yu., Zhou B. Ten alien chromosome additions of Gossypium hirsutum–Gossypium bickii developed by integrative uses of GISH and species-specific SSR markers. Mol Genet Genomics. 2018;293(4):1-11. doi 10.1007/s00438018 14345Ulloa M., Wang C., Saha S., Hutmacher R.B., Stelly D.M., Jen kins J.N., Burke J., Roberts P.A. Analysis of root-knot nematode and fusarium wilt disease resistance in cotton (Gossypium spp.) using chromosome substitution lines from two alien species. Genetica. 2016;144:167-179. doi 10.1007/s1070901698870Ulloa M., Wang C., Saha S., Hutmacher R.B., Stelly D.M., Jen kins J.N., Burke J., Roberts P.A. Analysis of root-knot nematode and fusarium wilt disease resistance in cotton (Gossypium spp.) using chromosome substitution lines from two alien species. Genetica. 2016;144:167-179. doi 10.1007/s1070901698870Wang P.Z., Bian L., Cao C. Mapping of nuclear male-sterile gene ms14 using SSR markers in cotton. Mol Plant Breed. 2016;7:35. doi 10.5376/mpb.2016.07.0035Wang P.Z., Bian L., Cao C. Mapping of nuclear male-sterile gene ms14 using SSR markers in cotton. Mol Plant Breed. 2016;7:35. doi 10.5376/mpb.2016.07.0035Wendel J.F. New World tetraploid cottons contain Old World cy toplasm. Proc Natl Acad Sci USA. 1989;86(11):4132-4136. doi 10.1073/pnas.86.11.4132Wendel J.F. New World tetraploid cottons contain Old World cy toplasm. Proc Natl Acad Sci USA. 1989;86(11):4132-4136. doi 10.1073/pnas.86.11.4132Yu Yu., Yuan D., Liang Sh., Li X., Wang X., Lin Zh., Zhang X. Genome structure of cotton revealed by a genomewide SSR genetic map constructed from a BC1 population between Gossypium hirsutum and G. barbadense. BMC Genomics. 2011;12:15. doi 10.1186/147121641215Yu Yu., Yuan D., Liang Sh., Li X., Wang X., Lin Zh., Zhang X. Genome structure of cotton revealed by a genomewide SSR genetic map constructed from a BC1 population between Gossypium hirsutum and G. barbadense. BMC Genomics. 2011;12:15. doi 10.1186/147121641215

The authors declare that there are no conflicts of interest present.