vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-133vavilov-4923Research ArticleМОЛЕКУЛЯРНЫЕ МАРКЕРЫ В ГЕНЕТИКЕ И СЕЛЕКЦИИBIOINFORMATICS AND SYSTEM BIOLOGYВалидация маркеров, разработанных для выявления локусов устойчивости к Pyrenophora teres f. teres на хромосомах ячменя 3Н, 4Н и 6Н при полигенном наследовании признакаValidation of markers for resistance to Pyrenophora teres f. teres loci on barley chromosomes 3H, 4H, and 6H in the polygenic inheritance of the traithttps://orcid.org/0000-0001-7368-0797АфанасенкоО. С.AfanasenkoO. S.

Санкт-Петербург

St. Petersburg

olga.s.afan@gmail.comhttps://orcid.org/0000-0003-3383-2973МироненкоН. В.MironenkoN. V.

Санкт-Петербург

St. Petersburg

ЛашинаН. М.LashinaN. M.

Санкт-Петербург

St. Petersburg

РозановаИ. В.RozanovaI. V.

Сочи

Sochi

КыроваЕ. И.KyrovaE. I.

Санкт-Петербург

St. Petersburg

НикольскаяЮ. С.NikolskayaYu. S.

Санкт-Петербург

St. Petersburg

ЗубковичА. А.ZubkovichA. A.

Жодино

Zhodino

Всероссийский научно-исследовательский институт защиты растений (ВИЗР)РоссияAll-Russian Research Institute of Plant Protection (VIZR)Russian FederationНаучно-технологический университет «Сириус», Центр генетики и наук о жизниРоссияSirius University of Science and Technology, Center of Genetics and Life SciencesRussian FederationНаучно-практический центр Национальной академии наук Беларуси по земледелиюБеларусьResearch and Practical Center of Agriculture of the National Academy of Sciences of BelarusBelarus20251101202629812351245Copyright © Афанасенко О.С., Мироненко Н.В., Лашина Н.М., Розанова И.В., Кырова Е.И., Никольская Ю.С., Зубкович А.А., 20262026Афанасенко О.С., Мироненко Н.В., Лашина Н.М., Розанова И.В., Кырова Е.И., Никольская Ю.С., Зубкович А.А.Afanasenko O.S., Mironenko N.V., Lashina N.M., Rozanova I.V., Kyrova E.I., Nikolskaya Y.S., Zubkovich A.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4923

Возбудитель сетчатой пятнистости листьев ячменя Pyrenophora teres Drechs. f. teres (Ptt) относится к эпифитотийно опасным патогенам ячменя. Разработка генетической защиты от этой болезни – важное звено ресурсосберегающих и экологически безопасных технологий возделывания ячменя. Для селекции на устойчивость к Ptt необходимы эффективные маркеры QTL, контролирующих как качественную, так и количественную устойчивость. При проведении полногеномного анализа (GWAS) нами выявлены образцы ячменя различного происхождения, SNP-гаплотипы которых ассоциировались с локусами устойчивости одновременно на разных хромосомах. Целью исследований была валидация SNP-маркеров локусов устойчивости к Ptt на хромосомах 3Н, 4Н и 6Н с использованием F2 популяции от скрещивания шести устойчивых образцов к-5900, к-8829, к-8877, к-14936, к-30341 и к-18552 с восприимчивым сортом Tatum. Расщепление по устойчивости во всех комбинациях скрещиваний подтвердило наличие нескольких генетических детерминант устойчивости у изучаемых образцов. Для изучения полиморфизма родительских компонентов скрещиваний и соответствия фенотипа наличию/отсутствию маркера в расщепляющихся популяциях были разработаны праймеры со специфичным 3’-концом, CAPS- и KASP-маркеры. Значимая связь (p < 0.05) наличия CAPS маркера JHI-Hv50k-2016-391380 HindIII на хромосоме 6Н и фенотипа устойчивости к Ptt у растений F2 выявлена в комбинациях скрещивания восприимчивого сорта Tatum с образцами к-5900, к-8829, к-8877 и к-18552; на хромосоме 4Н при фрагментном анализе значимая связь с фенотипом устойчивости в популяции F2 с участием образца к-8877 выявлена для маркера JHI-Hv50k-2016-237924, образца к-5900 для маркера SCRI_RS_181886 и образца к-8829 для маркера JHI-Hv50k-2016-166356. Наличие QTL на хромосоме 6Н, контролирующего качественную устойчивость у четырех образцов ячменя, маскирует проявление других генов с меньшим фенотипическим проявлением, что и является причиной несоответствия фенотипа устойчивости и наличия молекулярного маркера в расщепляющихся популяциях. Доноры устойчивости и молекулярные маркеры с доказанной эффективностью могут быть использованы в MAS для создания устойчивых к возбудителю сетчатой пятнистости сортов ячменя.

The causal agent of net blotch Pyrenophora teres Drechs. f. teres (Ptt) is a dangerous pathogen of barley. The development of genetic protection against this disease is a necessary link in resource-saving and environmentally friendly barley cultivation technologies. Effective QTL markers controlling both qualitative and quantitative resistance are required for breeding for resistance to Ptt. As a result of GWAS, we identified barley accessions of different origins, the SNP haplotypes of which were associated with resistance loci simultaneously on different barley chromosomes (VIR catalogue numbers: k-5900, k-8829, k-8877, k-14936, k-30341 and k-18552). The aim of the study was to validate SNP markers (MM) of Ptt resistance loci on chromosomes 3H, 4H and 6H in F2 from crossing six resistant accessions with the susceptible variety Tatum. The observed segregation for resistance in all crossing combinations confirmed the presence of several genetic determinants of resistance in the studied accessions. To study the polymorphism of the parents from the crosses and the correspondence between the phenotypes to the presence/absence of the markers in the segregating populations, primers with a specific 3’-end, CAPS markers, and KASP markers were developed. A significant association (p < 0.05) between the presence of the CAPS marker JHI-Hv50k-2016-391380 HindIII on chromosome 6H and the phenotype of resistance to Ptt in F2 plants was revealed in crosses between the susceptible cultivar Tatum and accessions k-5900, k-8829, k-8877 and k-18552. On chromosome 4H, a significant association with the resistance phenotype in the F2 population from the cross with accession k-8877 was revealed for marker JHI-Hv50k-2016-237924, and in that from the cross with accession k-5900, for marker SCRI_RS_181886. The presence of QTL on chromosome 6H, which controls qualitative resistance in four barley accessions, masks the expression of other genes, which explains the discrepancy between the resistance phenotype and the presence of molecular markers in the segregating populations. Resistance donors and molecular markers with proven efficacy can be used in marker-assisted selection (MAS) to develop barley cultivars resistant to net blotch.

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The authors declare that there are no conflicts of interest present.