vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-134vavilov-4924Research ArticleМОЛЕКУЛЯРНЫЕ МАРКЕРЫ В ГЕНЕТИКЕ И СЕЛЕКЦИИBIOINFORMATICS AND SYSTEM BIOLOGYВыявление нуклеотидного полиморфизма гена CtE1 и разработка KASP-маркеров для гуара (Cyamopsis tetragonoloba (L.) Taub.)Identification of CtE1 gene nucleotide polymorphisms and development of SNP-based KASP markers in guar (Cyamopsis tetragonoloba (L.) Taub.)https://orcid.org/0000-0001-7666-1508Криолло ДельгадоЛ.Criollo DelgadoL.

Москва

Moscow

https://orcid.org/0009-0001-3826-7697ЗевудеД.ZewudeD.

Москва

Moscow

https://orcid.org/0000-0002-2444-9502КаржаевД. С.KarzhaevD. S.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0001-9679-2791ПолевД. Е.PolevD. E.

Санкт-Петербург

St. Petersburg

https://orcid.org/0000-0002-2578-6279ПотокинаЕ. К.PotokinaE. K.

Москва

Moscow

e.potokina@skoltech.ruСколковский институт науки и технологий (Сколтех)РоссияSkolkovo Institute of Science and Technology (Skoltech)Russian FederationИнститут леса и природопользования, Санкт-Петербургский государственный лесотехнический университетРоссияInstitute of Forest and Environmental Management, Saint-Petersburg State Forest Technical UniversityRussian FederationЛаборатория метагеномных исследований, Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. ПастераРоссияLaboratory of Metagenomics Research, Saint-Petersburg Pasteur InstituteRussian Federation20251101202629812461254Copyright © Криолло Дельгадо Л., Зевуде Д., Каржаев Д.С., Полев Д.Е., Потокина Е.К., 20262026Криолло Дельгадо Л., Зевуде Д., Каржаев Д.С., Полев Д.Е., Потокина Е.К.Criollo Delgado L., Zewude D., Karzhaev D.S., Polev D.E., Potokina E.K.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4924

Гуар (Cyamopsis tetragonoloba (L.) Taub) – бобовое растение короткого дня, возделывание которого в высоких широтах ограничено из-за его чувствительности к длинному фотопериоду, что негативно влияет на цветение и созревание этой новой индустриально значимой сельскохозяйственной культуры. У ее близкого родственника – сои – ген E1 вовлечен в регуляцию процессов цветения в условиях длинного дня. В нашем исследовании естественное разнообразие гена CtE1 (гомолога E1)  проанализировано на выборке из 144 образцов гуара. Для этой цели ген CtE1 был амплифицирован и секвенирован с использованием Illumina, в результате чего идентифицировано пять новых SNP в 5’- и 3’-нетранслируемых областях, а также в кодирующей части гена CtE1. На основе выявленных SNP разработаны и протестированы пять KASP-маркеров, связанных с полиморфизмом целевого гена в изученной коллекции гуара. Один несинонимичный SNP был локализован в кодирующей области; этот полиморфизм приводит к консервативной аминокислотной замене Arg→Lys в кодируемом белке. Значимой связи между обнаруженными SNP и имеющимися данными об изменчивости сроков начала цветения или продолжительности вегетационного периода в выборке из 144 образцов не обнаружено. Полученные результаты свидетельствуют о том, что естественный нуклеотидный полиморфизм гена CtE1, представленный в изученной коллекции образцов гуара, не оказывает существенного влияния на цветение и созревание растений. Ограниченное функциональное аллельное разнообразие, выявленное в гене CtE1 гуара по сравнению с аллельным разнообразием гена E1 у сои, вероятно, отражает различия в их эволюционной истории и различные направления искусственного отбора в процессе селекции.

Guar (Cyamopsis tetragonoloba (L.) Taub), is an important short-day legume crop, whose cultivation is limited at high latitudes due its photoperiod sensitivity, that negatively impacts flowering and maturation of this industrial oriented crop. In its close relative, soybean, the E1 gene has been highly associated with the regulation of flowering time under long-day conditions. In this study we investigated the natural diversity of the E1 homologue gene (CtE1) in a panel of 144 guar accessions. For this purpose, the CtE1 gene was amplified and sequenced using Illumina. As a result, five novel SNPs were identified in the 5’-untranslated region, coding region, and 3’-untranslated region of the CtE1 gene. One non-synonymous SNP was located in the coding region causing a conservative Arg→Lys substitution. Based on the identified SNP, five KASP markers linked to polymorphism in the target gene were developed and tested in the guar collection. No significant associations were detected between discovered SNPs and available data on variability in flowering time or vegetation period length in the cohort of 144 accessions. These findings suggest that natural variation of the CtE1 gene in the studied germplasm collection has minimal effect on flowering or maturation. The limited functional allelic diversity observed in the CtE1 gene of guar compared to the E1 gene in soybean likely reflects differences in their evolutionary histories, domestication bottlenecks, and selection pressures.

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The authors declare that there are no conflicts of interest present.