vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-137vavilov-4927Research ArticleБИОИНФОРМАТИКА И СИСТЕМНАЯ БИОЛОГИЯBIOINFORMATICS AND SYSTEMS BIOLOGYИсследование инсектицидного и фунгицидного потенциала бактерий эндофитов пшеницы, сои и рапса методами биоинформатического анализаStudy of insecticidal and fungicidal potential of endophytic bacteria of wheat, soybean and rapeseed by bioinformatic analysis methodshttps://orcid.org/0000-0003-1729-7712ЛаховаТ. Н.LakhovaT. N.

Новосибирск

Novosibirsk

tlakhova@bionet.nsc.ruhttps://orcid.org/0000-0003-1877-9107КлименкоА. И.KlimenkoA. I.

Новосибирск

Novosibirsk

https://orcid.org/0000-0003-0929-6832ВасильевГ. В.VasilievG. V.

Новосибирск

Novosibirsk

https://orcid.org/0000-0003-2164-5935ГырнецЕ. Ю.GyrnetsE. Yu.

Краснодар

Krasnodar

https://orcid.org/0000-0002-0060-1995АсатуроваА. М.AsaturovaA. M.

Краснодар

Krasnodar

https://orcid.org/0000-0003-3138-381XЛашинС. А.LashinS. A.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университетРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationФедеральное государственное бюджетное научное учреждение «Федеральный научный центр биологической защиты растений»РоссияFederal State Budgetary Scientific Institution “Federal Research Center of Biological Plant Protection”Russian Federation20251101202629813041317Copyright © Лахова Т.Н., Клименко А.И., Васильев Г.В., Гырнец Е.Ю., Асатурова А.М., Лашин С.А., 20262026Лахова Т.Н., Клименко А.И., Васильев Г.В., Гырнец Е.Ю., Асатурова А.М., Лашин С.А.Lakhova T.N., Klimenko A.I., Vasiliev G.V., Gyrnets E.Y., Asaturova A.M., Lashin S.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4927

Эндофитные бактерии способны влиять на доступность различных соединений, урожайность и рост сельскохозяйственных растений, а также обеспечивать устойчивость к болезням и вредителям. Поэтому исследование эндофитов сельскохозяйственно значимых культурных растений является перспективной задачей в области биологической защиты растений. В данной работе рассмотрены изоляты штаммов бактерий, полученные из листьев и/или корней пшеницы, рапса и сои. Было произведено полногеномное секвенированиеизолятов. С помощью аналитического конвейера собраны и охарактеризованы геномы 15 штаммов бактерий эндофитов культурных растений, проанализирован их инсектицидный и фунгицидный потенциал. Анализ генного репертуара с помощью программы GenAPI показал высокую степень соответствия между генным репертуаром штамма BZR 585 относительно Alcaligenes phenolicus, BZR 762 и BZR 278 относительно Alcaligenes sp., BZR 588 и BZR 201P относительно Paenochrobactrum pullorum. Во всех штаммах, за исключением BZR 162, BZR 588 и BZR 201P, найдены гены, кодирующие белки, обладающие фунгицидной активностью, такие как итурины, фенгицины и сурфактины. Также во всех штаммах найдены гены, кодирующие белки с инсектицидной активностью, а именно: GroEL, Spp1Aa1, Spp1Aa2, Vpb1Ab1, Vpb4Ca1, HldE, фенгицин, микосубтилин и бацилломицин. Полученные геномные данные подтверждены экспериментальными испытаниями: ранее показана высокая инсектицидная активность штаммов BZR 1159, BZR 936, BZR 920 и др. против Galleria mellonella, Tenebrio molitor и Cydia pomonella, а также фунгицидные свойства против Fusarium, Alternaria, Trichothecium. Это демонстрирует практическую значимость выявленных генетических детерминант для создания новых агентов биоконтроля.

Endophytic bacteria play a key role in agricultural ecosystems, as they can affect the availability of various compounds, crop yield and growth, and provide resistance to diseases and pests. Therefore, the study of endophytes of agriculturally important crop plants is a promising task in the field of biological plant protection. Understanding the mechanisms of interaction between endophytic bacteria and plants will allow the use of these microorganisms as bioagents in the future and thus reduce dependence on chemical pesticides. In this paper, samples obtained from the leaves and/or roots of wheat, rapeseed and soybean are considered. Whole-genome sequencing of the isolates was performed. Using an analytical pipeline, the genomes of 15 strains of endophyte bacteria of cultivated plants were assembled and characterized. Their insecticidal and fungicidal potential was analyzed. Gene repertoire analysis performed with GenAPI showed a high degree of correspondence between the gene repertoires of strain BZR 585 against Alcaligenes phenolicus, BZR 762 and BZR 278 against Alcaligenes sp., BZR 588 and BZR 201P against Paenochrobactrum pullorum. All strains, with the exception of BZR 162, BZR 588 and BZR 201P, were found to contain genes encoding proteins with fungicidal activity, such as iturins, fengycins and surfactins. All strains also contained genes encoding proteins with insecticidal activity, namely GroEL, Spp1Aa1, Spp1Aa2, Vpb1Ab1, Vpb4Ca1, HldE, mycosubtilin, fengycin and bacillomycin. The obtained genomic data are confirmed by the results of previous experimental studies: high insecticidal activity of a number of strains (BZR 1159, BZR 936, BZR 920, etc.) against Galleria mellonella, Tenebrio molitor and Cydia pomonella, as well as fungicidal properties against Fusarium, Alternaria, Trichothecium, was demonstrated. This shows the practical significance of the identified genetic determinants for the creation of new biocontrol agents. 

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The authors declare that there are no conflicts of interest present.