vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-25-138vavilov-4928Research ArticleКОМПЬЮТЕРНАЯ ГЕНОМИКА ЖИВОТНЫХ И ЧЕЛОВЕКАCOMPUTATIONAL GENOMICS OF ANIMALS AND THE HUMANПоиск генов домашнего хозяйства для анализа изменения экспрессии отдельных генов у Macaca mulattaA housekeeping gene search to analyze expression changes of individual genes in Macaca mulattahttps://orcid.org/0000-0003-1274-2901ШульскаяМ. В.ShulskayaM. V.

 Москва

Moscow

shulskaya.m@yandex.ruhttps://orcid.org/0000-0002-6200-4491АлиеваА. Х.AlievaA. Kh.

 Москва

Moscow

https://orcid.org/0009-0007-2781-2649КумаковИ. Р.KumakovI. R.

 Москва

Moscow

https://orcid.org/0000-0002-4544-6183ШадринаМ. И.ShadrinaM. I.

 Москва

Moscow

https://orcid.org/0000-0003-3530-0655СломинскийП. А.SlominskyP. A.

 Москва

Moscow

Национальный исследовательский центр «Курчатовский институт»РоссияNational Research Center “Kurchatov Institute”Russian Federation20251101202629813181324Copyright © Шульская М.В., Алиева А.Х., Кумаков И.Р., Шадрина М.И., Сломинский П.А., 20262026Шульская М.В., Алиева А.Х., Кумаков И.Р., Шадрина М.И., Сломинский П.А.Shulskaya M.V., Alieva A.K., Kumakov I.R., Shadrina M.I., Slominsky P.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4928

Макаки резус (Macaca mulatta) являются наиболее распространенными нечеловекообразными приматами, их используют в качестве модельных объектов, в первую очередь, из-за филогенетической и физиологической близости к человеку. В настоящее время модельные организмы широко используются для целого ряда исследований, в том числе на уровне транскриптома. При этом для анализа экспрессии отдельных генов применяется универсальный метод – полимеразная цепная реакция в реальном времени. Проведение такого рода исследований всегда требует предварительного подбора «генов домашнего хозяйства» (ГДХ) – генов, необходимых для реализации основных функций в клетке и стабильно экспрессирующихся в различных типах клеток и при разных условиях. На сегодняшний день для макак резус существуют лишь две систематизированные работы по поиску ГДХ, однако эти исследования проводились лишь для тканей мозга и не учитывают такой важный критерий, как связь ГДХ с заболеваниями. В связи с этим в нашей работе были сформулированы ключевые критерии, учитываемые при подборе ГДХ. Проведены поиск и систематизация кандидатных ГДХ. В результате сформированы две панели перспективных ГДХ для M. mulatta: расширенная панель на 56 генов и малая панель, состоящая из восьми генов: ARHGDIA, CYB5R1, NDUFA7, RRAGA, TTC1, UBA6, VPS72 и YWHAH. Обе панели соответствуют всем критериям подбора ГДХ (не имеют псевдогенов ни у макаки, ни у человека, характеризуются стабильной и достаточной экспрессией в мозге макак резус и могут быть использованы для анализа экспрессии не только в мозге, но и в периферической крови). Однако необходимо отметить, что данные экспериментально не верифицированы и требуют проверки в лабораторных условиях.

Rhesus macaques (Macaca mulatta) are the most common non-human primates living in captivity. The use of rhesus macaques as model objects is determined, first of all, by their phylogenetic and physiological closeness to humans, and, as a consequence, the possibility of extrapolating the obtained results to humans. Currently, it is known that a number of biochemical changes occur under various physiological conditions, including at the transcriptomic level. The real-time polymerase chain reaction is a widely used universal method for gene expression analysis. Carrying out such studies always requires a preliminary selection of “housekeeping genes” (HKGs) – genes necessary for the implementation of basic functions in the cell and stably expressed in different cell types and under different conditions. At present, there are only two systematic studies on the search for HKGs in the rhesus macaque brain, and therefore in this work a search and systematization of HKGs for this species were carried out. As a result, two panels of promising HKGs for M. mulatta were formed: an extended panel, consisting of 56 genes, and a small panel, consisting of 8 genes: ARHGDIA, CYB5R1, NDUFA7, RRAGA, TTC1, UBA6, VPS72, and YWHAH. Both panels of potential HKGs do not have pseudogenes in macaques or humans, are characterized by stable and sufficient expression in the brain of rhesus macaques and can be used to analyze expression not only in the brain but also in peripheral blood. However, it should be noted that the data have not been experimentally verified and require verification in laboratory conditions. 

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The authors declare that there are no conflicts of interest present.