vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-26-14vavilov-4978Research ArticleГЕНЕТИКА ЖИВОТНЫХANIMAL GENETICSПаттерн распределения Р-транспозона и blood ретротранспозона в отводках референсных линий Harwich и Canton-S из разных лабораторий не влияет на проявление внутривидового PM гибридного дисгенеза у Drosophila melanogasterDifferent patterns of P transposon and blood retrotransposon distribution in Harwich and Canton-S sub-strains do not affect the manifestation of Drosophila melanogaster intraspecific PM hybrid dysgenesishttps://orcid.org/0000-0002-6341-8522ЗахаренкоЛ. П.ZakharenkoL. P.

Новосибирск

Novosibirsk

zakharlp@bionet.nsc.ruhttps://orcid.org/0000-0002-2691-3241ИлинскийЮ. Ю.IlinskyY. Y.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian Federation2026050320263013642Copyright © Захаренко Л.П., Илинский Ю.Ю., 20262026Захаренко Л.П., Илинский Ю.Ю.Zakharenko L.P., Ilinsky Y.Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/4978

Внутривидовое бесплодие, природа которого не всегда понятна, встречается у многих эукариот. Внутривидовой PM гибридный дисгенез (РМ ГД) у Drosophila melanogaster проявляется в одном из направлений скрещивания в бесплодии потомства и некоторых других генетических нарушениях в результате несовместимости между материнской цитоплазмой и отцовским геномом. Считается, что PM  ГД является результатом массового перемещения P-элемента, если материнская цитоплазма не имеет репрессора, блокирующего его перемещение. В данной работе мы исследовали распределение P-элемента в отводках референсных для PM  ГД линиях (Canton-S и Harwich), которые долгое время содержались в разных лабораториях. Паттерны распределения P-элемента различались в разных отводках Harwich. Скорость перемещения P-элемента, не индуцированная скрещиваниями, сопоставима со скоростью перемещения других ДНК-транспозонов. Паттерн распределения малоактивного ретротранспозона blood в производных линии Harwich был более стабильным по сравнению с Р-элементом, что свидетельствует о генетическом родстве между отводками. В некоторых отводках линии Canton-S были обнаружены следы P-элемента, что может указывать на генетическое загрязнение. Значительная разница в паттерне распределения транспозона blood в отводках линии Canton-S также  говорит о генетической гетерогенности отводков. Несмотря на сложную генеалогию отводков исследованных линий, включающую случаи возможного генетического загрязнения, и различия в распределении P-элементов, способность проявлять симптомы PM  ГД у исследованных отводков сохраняется. Мы показали, что все изученные производные Canton-S имеют сильный M-цитотип, а все отводки Harwich – индуцирующий P-цитотип.

Intraspecific infertility, the nature of which is not always understood, occurs in many eukaryotes. Intraspecific PM hybrid dysgenesis (PM HD) in Drosophila melanogaster manifests in one cross direction as offspring infertility and other genetic disorders due to incompatibility between the maternal cytoplasm and the paternal genome. PM HD is believed to result from a massive transposition of the P-element when the maternal cytoplasm lacks a repressor to block it. In this work, we have investigated the distribution of the P transposon and blood retrotransposon in the reference PM HD strains (Canton-S and Harwich), which have been maintained in different laboratories for several decades. P-element distribution patterns vary among Harwich sub-strains, indicating that the P-element was translocated in these genomes. The rate of movement of the P-element, which was not induced by crosses, is comparable to the rate of movement of other DNA transposons. The distribution pattern of the low-active blood retrotransposon in Harwich sub-strains is more stable than that of the P-element, indicating genetic relatedness between sub-strains. Derivatives of the P-element detected in some Canton-S sub-strains possibly indicate genetic contamination. The significant difference in the blood transposable element distribution pattern in Canton-S sub-strains also indicates genetic heterogeneity among them. Despite the complex genealogy of the studied sub-strains, including cases of possible genetic contamination, and differences in P-element distribution, the ability to express PM HD symptoms is preserved in the studied sub-strains.

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The authors declare that there are no conflicts of interest present.