vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-50Research ArticleСтатьиArticlesПОДАВЛЕНИЕ РЕПЛИКАЦИИ СУБГЕНОМНОГО РНК РЕПЛИКОНА ВИРУСА ГЕПАТИТА С ИНГИБИТОРОМ NS3 ПРОТЕАЗЫ SCH 503034 В Huh-7 КЛЕТКАХ: СТОХАСТИЧЕСКАЯ МОДЕЛЬSUPPRESSION OF SUBGENOMIC HEPATITIS C VIRUS RNA REPLICON REPLICATION IN Huh-7 CELLS BY THE NS3 PROTEASE INHIBITOR SCH5030334: A STOCHASTIC MATHEMATICAL MODELМищенкоЕ. Л.MishchenkoE. L.elmish@bionet.nsc.ruИванисенкоН. В.IvanisenkoN. V.elmish@bionet.nsc.ruАкбердинИ. Р.AkberdinI. R.elmish@bionet.nsc.ruДеменковП. С.DemenkovP. S.elmish@bionet.nsc.ruЛихошвайВ. А.LikhoshvaiV. A.elmish@bionet.nsc.ruКолчановН. А.KolchanovN. A.elmish@bionet.nsc.ruИванисенкоВ. А.IvanisenkoV. A.elmish@bionet.nsc.ruФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, РоссияРоссияInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaRussian FederationФедеральное государственное бюджетное учреждение науки Институт цитологии и генетики Сибирского отделения РАН, Новосибирск, Россия Новосибирский государственный университет, Новосибирск, РоссияРоссияInstitute of Cytology and Genetics, SB RAS, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, RussiaRussian Federation201212122014162339347Copyright © Мищенко Е.Л., Иванисенко Н.В., Акбердин И.Р., Деменков П.С., Лихошвай В.А., Колчанов Н.А., Иванисенко В.А., 20142014Мищенко Е.Л., Иванисенко Н.В., Акбердин И.Р., Деменков П.С., Лихошвай В.А., Колчанов Н.А., Иванисенко В.А.Mishchenko E.L., Ivanisenko N.V., Akberdin I.R., Demenkov P.S., Likhoshvai V.A., Kolchanov N.A., Ivanisenko V.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/50

Вирус гепатита С (ВГС) вызывает опасное заболевание печени, часто сопровождающееся циррозом и гепатоклеточной карциномой. Против ВГС до сих пор нет эффективной терапии. Для предсказания эффективности действия новых потенциальных лекарств, направленных против ВГС, актуально применение математического моделирования. Впервые предложена стохастическая модель репликации субгеномного РНК репликона ВГС в Huh-7 клетках в присутствии ингибитора NS3 протеазы ВГС SCH 503034. Модель описывает экспериментально полученные кинетики подавления вирусной РНК в присутствии разных концентраций SCH 503034 и может применяться для разработки оптимальных стратегий подавления РНК ВГС в Huh-7 клетках.

Hepatitis C virus (HCV) causes a severe liver disease frequentlyassociatedwith cirrhosis andhepatocellular carcinoma. No effective anti-HCVtherapyis available so far. Thus, it is pertinent to applymathematical modelingto prediction ofthe efficiencyofnew candidate pharmaceuticals. Astochastic mathematical model for subgenomic HCVreplicon replication in Huh-7 cells with the presence ofthe HCVNS3 protease inhibitor SCH 503034 is proposed. The model describes the experimental kinetic curves ofviral RNAsuppression at various SCH 503034 concentrations. It is applicable to the development ofapproaches to HCVRNAsuppression in Huh-7 cells.

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The authors declare that there are no conflicts of interest present.