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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJ15.140</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-502</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Актуальные технологии генетики и селекции. ОБЗОР</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Current technologies in genetics and breeding. REVIEW</subject></subj-group></article-categories><title-group><article-title>Перспективные возможности использования молекулярно-генетических подходов для управления технологическими свойствами зерна пшеницы в контексте цепочки «зерно – мука – хлеб»</article-title><trans-title-group xml:lang="en"><trans-title>Prospects of molecular genetic approaches in controlling technological properties of wheat grain in the context of the "grain – flour – bread" chain</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хлесткина</surname><given-names>Е. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Khlestkina</surname><given-names>E. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><email xlink:type="simple">khlest@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Пшеничникова</surname><given-names>Т. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Pshenichnikova</surname><given-names>T. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><email xlink:type="simple">khlest@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Усенко</surname><given-names>Н. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Usenko</surname><given-names>N. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><email xlink:type="simple">khlest@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Отмахова</surname><given-names>Ю. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Otmakhova</surname><given-names>Yu. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новосибирск</p></bio><bio xml:lang="en"><p>Novosibirsk</p></bio><email xlink:type="simple">khlest@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-4"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»;&#13;
Федеральное государственное автономное образовательное учреждение высшего образования «Новосибирский национальный исследовательский&#13;
государственный университет»<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics SB RAS;&#13;
Novosibirsk State University<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics SB RAS<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru">Федеральное государственное автономное образовательное учреждение высшего образования «Новосибирский национальный исследовательский государственный университет»<country>Россия</country></aff><aff xml:lang="en">Novosibirsk State University<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru">Федеральное государственное автономное образовательное учреждение высшего образования «Новосибирский национальный исследовательский&#13;
государственный университет»;&#13;
Федеральное государственное бюджетное учреждение науки «Институт экономики и организации промышленного производства Сибирского отделения Российской академии наук»<country>Россия</country></aff><aff xml:lang="en">Novosibirsk State University;&#13;
Institute of Economics and Industrial Engineering SB RAS<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="epub"><day>29</day><month>01</month><year>2016</year></pub-date><volume>20</volume><issue>4</issue><fpage>511</fpage><lpage>527</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Хлесткина Е.К., Пшеничникова Т.А., Усенко Н.И., Отмахова Ю.С., 2016</copyright-statement><copyright-year>2016</copyright-year><copyright-holder xml:lang="ru">Хлесткина Е.К., Пшеничникова Т.А., Усенко Н.И., Отмахова Ю.С.</copyright-holder><copyright-holder xml:lang="en">Khlestkina E.K., Pshenichnikova T.A., Usenko N.I., Otmakhova Y.S.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/502">https://vavilov.elpub.ru/jour/article/view/502</self-uri><abstract><p>В рамках данной обзорной статьи рассмотрены возможности молекулярно-генетических подходов к управлению технологическими свойствами зерна пшеницы, влияющими на качество готовой продукции хлебопекарного производства. В настоящее время при росте производства зерна происходит вымывание традиционного ассортимента, ухудшается качество хлеба массовых сортов, в хлебопечении в виде улучшителей применяются десятки различных веществ биологического и химического происхождения. Между тем генетический потенциал пшеницы позволяет создавать сорта для производства зерна с технологическими характеристиками, пригодными для производства высококачественного хлеба. В истории отечественной селекции имеются множественные примеры создания сортов для получения зерна 1-го и 2-го класса, а современная молекулярная генетика предлагает подходы, которые при комбинировании с традиционными методами селекции делают возможным ускоренное создание новых адаптированных к условиям и требованиям хлебопекарной отрасли сортов за счет использования естественного генетического потенциала пшеницы. Авторами обобщены данные по разнообразию требований, предъявляемых к зерну и муке различного конечного пищевого использования. Проанализированы статистические данные по объемам и структуре качества зерна в России в период 2011–2014 гг. Выявлена существенная деформация структуры качества производимого зерна пшеницы в пользу менее ценных классов. Проведен краткий ретроспективный анализ исследований в области генетики пшеницы, показавших роль генетических факторов в формировании технологических свойств зерна и муки. Рассмотрены различные подходы для ускоренной селекции сортов с заданными свойствами в перспективе развития исследований в области молекулярной генетики растений. Приведены примеры, иллюстрирующие возможность и целесообразность использования методов ДНК-диагностики на разных этапах процесса, в ходе которого реализуется и сказывается на качестве конечного продукта заложенный в генофонде продовольственных культур генетический потенциал. Рассмотрены результаты молекулярно-генетических исследований по определению локализации и структуры генов, вовлеченных в формирование технологических свойств зерна пшеницы: содержание белка и сырой клейковины, мукомольные свойства, реологические свойства муки и теста, цвет муки, свойства крахмала. Суммированы данные о диагностических ДНК-маркерах, подходящих для эффективного отбора генотипов взамен трудоемкого анализа технологических характеристик на промежуточных этапах селекции. Таким образом, в обобщенном виде представлена информация о генетическом потенциале мягкой пшеницы и современных технологических подходах, которые составляют основу для смены направления от повышенной химизации в сторону более мягкого и органичного влияния на качество основного сырья и продукции во всей продовольственной цепочке «зерно – мука – хлеб». </p></abstract><trans-abstract xml:lang="en"><p>The potential of molecular approaches in the management of the technological properties of wheat grain affecting the quality of the end product of bread industry is considered. Currently, along with the growth of grain production, the traditional range of staples is crowded out, the quality of most popular bread varieties deteriorates, and dozens of different substances of biological and chemical origin are used as bread improvers. Meanwhile, the genetic potential of wheat allows the development of varieties with technological parameters of grain suitable for production of high-quality bread. In Russia, multiple examples of the creation of varieties for the production of first and second-class grain are known, and modern molecular genetics offers techniques that can supplement classical breeding approaches and accelerate the development of new varieties adapted to the conditions and requirements of the baking industry, using the natural genetic potential of wheat. We summarize the diversity of requirements for grain and flour for different end-use products. Statistics on the volume and structure of grain quality in Russia in 2011–2014 is analyzed. An essential deformation of the quality structure of the produced wheat grain in favor of less valuable classes is observed. A brief retrospective analysis of research in wheat genetics, demonstrating the contribution of genetic factors to grain and flour technological properties, is performed. Various approaches to rapid breeding of varieties with desired properties are considered in relation with the development of plant molecular genetics. The paper provides examples illustrating the feasibility of using methods of DNA diagnostics in various stages of the process, during which the genetic potential of food crops expresses itself and affects the quality of the end product. The results of molecular studies on the localization and isolation of genes determining technological properties (protein and wet gluten contents, milling properties, rheological properties, flour color, and starch properties) are reviewed. The data on diagnostic DNA markers, which are suitable for efficient selection of genotypes instead of the time-consuming analysis of technological properties during breeding process, are summarized. Thus, the information about the genetic potential of bread wheat and modern technologies that provide grounds for moving from excessive use of chemicals to a more benign and organic effect on the quality of the products throughout the "grain – flour – bread" chain, is summarized. </p></trans-abstract><kwd-group xml:lang="ru"><kwd>мягкая пшеница</kwd><kwd>продовольственная безопасность</kwd><kwd>зерно</kwd><kwd>содержание клейковины</kwd><kwd>мукомольные свойства</kwd><kwd>мука</kwd><kwd>физические свойства теста</kwd><kwd>хлебопекарная промышленность</kwd><kwd>гены</kwd><kwd>ДНК-маркеры</kwd><kwd>геномная селекция</kwd><kwd>маркер-ориентированная селекция</kwd></kwd-group><kwd-group xml:lang="en"><kwd>bread wheat</kwd><kwd>food security</kwd><kwd>grain</kwd><kwd>gluten content</kwd><kwd>milling properties</kwd><kwd>flour</kwd><kwd>physical properties of the dough</kwd><kwd>baking industry</kwd><kwd>genes</kwd><kwd>DNA-markers</kwd><kwd>genomic selection</kwd><kwd>marker-assisted selection</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>средства федерального бюджета на выполнение государственного задания (проект № 0324-2015-0005)</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Беспалова Л.А., Васильев А.В., Аблова И.Б., Филобок В.А., Худокормова Ж.Н., Давоян Р.О., Давоян Э.Р., Карлов Г.И., Соловьев А.А., Дивашук М.Г., Майер Н.К., Дудников М.В., Мироненко Н.В., Баранова О.А. 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