vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-26-39vavilov-5102Research ArticleМОЛЕКУЛЯРНАЯ И КЛЕТОЧНАЯ БИОЛОГИЯMOLECULAR AND CELL BIOLOGYКонцепция природной реконструкции генома. Часть 5. Анализ изменения продолжительности жизни старых животных после реинфузии клеток костного мозга старых животных, обработанных hDNAgr и ангиогенином рекомбинантным человеческимConcept of natural genome reconstruction. Part 5. Analysis of changes in the lifespan of old animals after reinfusion of bone marrow cells derived from old animals and treated with hDNAgr in combination with recombinant human angiogeninРузановаВ. С.RuzanovaV. S.

Новосибирск

Novosibirsk

ГривцоваЛ. Ю.GrivtsovaL. U.

Обнинск

Obninsk

ОшихминаС. Г.OshikhminaS. G.

Новосибирск

Novosibirsk

ПроскуринаА. С.ProskurinaА. S.

Новосибирск

Novosibirsk

РиттерГ. С.RitterG. S.

Новосибирск

Novosibirsk

ДолговаЕ. В.DolgovaE. V.

Новосибирск

Novosibirsk

КириковичС. С.KirikovichS. S.

Новосибирск

Novosibirsk

ЛевитесЕ. В.LevitesE. V.

Новосибирск

Novosibirsk

ЕфремовЯ. Р.EfremovY. R.

Новосибирск

Novosibirsk

ДубатоловаТ. Д.DubatolovaT. D.

Новосибирск

Novosibirsk

МещаниноваМ. И.MeschaninovaM. I.

Новосибирск

Novosibirsk

МамаевА. Л.MamaevA. L.

Новосибирск

Novosibirsk

ТарановО. С.TaranovO. S.

р.п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

СидоровС. В.SidorovS. V.

Новосибирск

Novosibirsk

ЛеплинаО. Ю.LeplinaO. Y.

Новосибирск

Novosibirsk

ОстанинА. А.OstaninA. A.

Новосибирск

Novosibirsk

ЧерныхЕ. Р.ChernykhE. R.

Новосибирск

Novosibirsk

КолчановН. А.KolchanovN. A.

Новосибирск

Novosibirsk

БрюховецкийА. С.BryukhovetskiyA. S.

Москва

Moscow

БогачевС. С.BogachevS. S.

Новосибирск

Novosibirsk

ussialabmolbiol@mail.ruФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian FederationНациональный медицинский исследовательский центр радиологии Министерства здравоохранения Российской ФедерацииРоссияNational Medical Research Radiological Centre of the Ministry of Health of the Russian FederationRussian FederationИнститут молекулярной и клеточной биологии Сибирского отделения Российской академии наукРоссияInstitute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of SciencesRussian FederationИнститут химической биологии и фундаментальной медицины Сибирского отделения Российской академии наукРоссияInstitute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of SciencesRussian FederationООО «Лаборатория Ангиофарм»РоссияLaboratory Angiopharm LLCRussian FederationГосударственный научный центр вирусологии и биотехнологии «Вектор» РоспотребнадзораРоссияState Scientific Center of Virology and Biotechnology “Vector” of RospotrebnadzorRussian FederationНовосибирский национальный государственный исследовательский университет; Городская клиническая больница № 1РоссияNovosibirsk State University; City Clinical Hospital No. 1Russian FederationНаучно-исследовательский институт фундаментальной и клинической иммунологииРоссияResearch Institute of Fundamental and Clinical ImmunologyRussian FederationАО Клинический госпиталь «НейроВита»РоссияClinical Hospital ‘‘NeuroVita’’Russian Federation202626052026303345361Copyright © Рузанова В.С., Гривцова Л.Ю., Ошихмина С.Г., Проскурина А.С., Риттер Г.С., Долгова Е.В., Кирикович С.С., Левитес Е.В., Ефремов Я.Р., Дубатолова Т.Д., Мещанинова М.И., Мамаев А.Л., Таранов О.С., Сидоров С.В., Леплина О.Ю., Останин А.А., Черных Е.Р., Колчанов Н.А., Брюховецкий А.С., Богачев С.С., 20262026Рузанова В.С., Гривцова Л.Ю., Ошихмина С.Г., Проскурина А.С., Риттер Г.С., Долгова Е.В., Кирикович С.С., Левитес Е.В., Ефремов Я.Р., Дубатолова Т.Д., Мещанинова М.И., Мамаев А.Л., Таранов О.С., Сидоров С.В., Леплина О.Ю., Останин А.А., Черных Е.Р., Колчанов Н.А., Брюховецкий А.С., Богачев С.С.Ruzanova V.S., Grivtsova L.U., Oshikhmina S.G., Proskurina А.S., Ritter G.S., Dolgova E.V., Kirikovich S.S., Levites E.V., Efremov Y.R., Dubatolova T.D., Meschaninova M.I., Mamaev A.L., Taranov O.S., Sidorov S.V., Leplina O.Y., Ostanin A.A., Chernykh E.R., Kolchanov N.A., Bryukhovetskiy A.S., Bogachev S.S.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/5102

Проведены две серии тестов (на мышах и крысах) по оценке продолжительности жизни старых животных, которым были реинфузированы клетки костного мозга старых животных, обработанные фрагментированной ДНК человека (hDNAgr), ангиогенином рекомбинантным человеческим и двумя препаратами совместно. В качестве групп сравнения использовались животные, которым реинфузировали необработанные клетки костного мозга старых животных или клетки костного мозга от молодых особей. В случае как аутбредных мышей, так и мышей линии CBA/Lac не выявлено достоверного увеличения продолжительности жизни животных при реинфузии клеток костного мозга, обработанных препаратом hDNAgr, по сравнению с группой мышей, которым реинфузировали необработанные клетки костного мозга. При использовании линии CBA/Lac мыши, которым реинфузировали клетки костного мозга, обработанные ангиогенином, на 10-й месяц после обработки одновременно пали от характерного симптомокомплекса. Патоморфологический анализ предполагает, что одновременная гибель мышей произошла в результате патологических нарушений в выделительных системах животных. Реинфузия клеток костного мозга от старых животных, обработанных ангиогенином и hDNAgr, и клеток костного мозга, взятых от молодых животных, значительно увеличивает продолжительность жизни мышей в группах. Совместное применение двух активаторов, ангиогенина и hDNAgr, увеличивало среднюю продолжительность жизни 30 % экспериментальных мышей до 35 мес. при 28 мес. в контроле. При использовании в качестве модельных животных крыс линии Вистар в первом эксперименте было показано достоверное увеличение жизни крыс при реинфузии клеток костного мозга старых животных, обработанных препаратом hDNAgr, до 28 мес. по сравнению с группой, получавшей необработанные клетки костного мозга старых животных, где средняя продолжительность жизни крыс составила 24 мес. Во втором аналогичном эксперименте достоверной разницы в продолжительности жизни крыс для указанных двух групп показано не было. Животные, которым вводили клетки костного мозга, обработанные ангиогенином, прожили достоверно дольше, чем крысы из контрольной группы. Проведенный анализ количества теломерной ДНК в клетках костного мозга крыс экспериментальных и контрольной групп через 12 мес. после обработки свидетельствовал, что достоверного увеличения теломерной ДНК не произошло. Рассматривается молекулярная/клеточная модель старения организма, связанная с концепцией «природной реконструкции генома».

Two series of tests were performed, on mice and rats, to assess the lifespan of old animals reinfused with bone marrow cells from old animals treated with fragmented human DNA (hDNAgr), recombinant human angiogenin, and both preparations together. Animals reinfused with untreated bone marrow cells from old animals or bone marrow cells from young animals were used as comparison groups. Using both outbred mice and CBA/Lac mice, no significant increase in the lifespan of animals reinfused with bone marrow cells treated with the hDNAgr was found compared with the group of mice reinfused with untreated bone marrow cells. Using the CBA/Lac line, mice reinfused with bone marrow cells treated with angiogenin simultaneously died of the characteristic symptom complex at 10 months after treatment. Pathomorphological analysis suggests that the simultaneous death of mice occurred as a result of pathological disorders in the excretory systems of animals. Reinfusion of bone marrow cells from old animals treated with angiogenin and hDNAgr and bone marrow cells taken from young animals significantly increases the lifespan of mice in groups. The combined use of two activators, angiogenin and hDNAgr, increased the average lifespan of 30 % of experimental mice to 35 months compared to 28 months in the control. Using Wistar rats as model animals in the first experiment, a reliable increase in the lifespan of rats with reinfusion of bone marrow cells from old animals treated with the hDNAgr preparation to 28 months was shown compared to the group that received untreated bone marrow cells from old animals, where the average lifespan of rats was 24 months. In the second similar experiment, no reliable difference in the lifespan of rats for the two groups was shown. Animals injected with bone marrow cells treated with angiogenin lived significantly longer than rats from the control group. The analysis of the amount of telomeric DNA in bone marrow cells of rats from the experimental and control groups 12 months after treatment showed that there was no significant increase in telomeric DNA. A molecular/cellular model of aging of the organism associated with the concept of “natural reconstruction of the genome” is considered.

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The authors declare that there are no conflicts of interest present.