vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/vjgb-26-89vavilov-5295Research ArticleФИЗИОЛОГИЧЕСКАЯ ГЕНЕТИКАPHYSIOLOGICAL GENETICSВлияние отбора по поведению на уровень глюкозы в крови у серебристо-черных лисиц после рестрикционного стрессаEffect of selection for behavior on the blood glucose level in foxes exposed to restriction stressГулевичР. Г.GulevichR. G.

Новосибирск

Novosibirsk

gulevich@bionet.nsc.ruШихевичС. Г.ShikhevichS. G.

Новосибирск

Novosibirsk

ШепелеваД. В.ShepelevaD. V.

Новосибирск

Novosibirsk

ХарламоваА. В.KharlamovaA. V.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesRussian Federation202613102026306875882Copyright © Гулевич Р.Г., Шихевич С.Г., Шепелева Д.В., Харламова А.В., 20262026Гулевич Р.Г., Шихевич С.Г., Шепелева Д.В., Харламова А.В.Gulevich R.G., Shikhevich S.G., Shepeleva D.V., Kharlamova A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/5295

Известно, что отбор серебристо-черных лисиц по отношению к человеку влияет на гипоталамо-гипофизарно-надпочечниковую (ГГН) и симпатическую нервную системы, которые, в свою очередь, участвуют в регуляции углеводного обмена при стрессе. Наряду с этим показано влияние генетических факторов на углеводный обмен и толерантность к глюкозе. Оставалось неизвестным, влияет ли отбор по отношению к человеку на уровень глюкозы в крови у серебристо-черных лисиц. В данной работе исследовали базальный уровень глюкозы в крови у ручных, агрессивных и неселекционированных самцов и самок серебристо-черных лисиц, а также уровень глюкозы после рестрикционного стресса у самок. Полученные результаты свидетельствуют о достоверно более низком базальном уровне глюкозы в крови у ручных лисиц по сравнению с агрессивными и неселекционированными животными, независимо от пола. Кроме того, у неселекционированных самцов этот 

показатель был ниже по сравнению с агрессивными животными. У агрессивных лисиц найдены половые различия по базальному уровню глюкозы в крови: у самцов этот показатель выше, чем у самок. В течение всего исследуемого периода после стресса (через 10 мин и 2 ч) уровень глюкозы в крови у агрессивных самок оставался повышенным по сравнению с ручными. Спустя 2 ч после стресса только у агрессивных самок отмечали более высокий уровень глюкозы по сравнению с исходным уровнем. В суммарной выборке самок лисиц, независимо от поведения до и после стресса, найдена отрицательная корреляция между уровнем глюкозы в крови и баллом поведения, которым оценивали реакцию на человека. Для сравнения скорости поглощения глюкозы из общего кровотока у самок серебристо-черных лисиц, селекционируемых по поведению, проводили глюкозотолерантный тест (ГТТ). В целом, судя по показателям площади под кривыми ГТТ, отбор по поведению не влиял на толерантность к глюкозе, вместе с тем у агрессивных самок, в отличие от ручных и неселекционированных, в начале теста отмечали более продолжительный период повышенного уровня глюкозы в крови. Таким образом, отбор по поведению влияет на уровень глюкозы в крови у серебристо-черных лисиц независимо от пола в состоянии покоя, а у самок – и после стресса.

Selection of silver foxes for attitude to humans influences the hypothalamus-pituitary-adrenal axis and sympathetic nervous system, which, in turn, are involved in the regulation of carbohydrate metabolism under stress. It has also been shown that heredity affectes carbohydrate metabolism and glucose tolerance. It remains to find out whether the selection for attitude to humans affected carbohydrate metabolism and blood glucose levels in silver foxes. We studied basal blood glucose levels in tame, aggressive, and unselected male and female silver foxes and glucose levels in females exposed to stress. Our results indicate that the basal blood glucose level in tame foxes is significantly lower than in unselected or aggressive ones regardless of sex. In unselected males, this parameter was lower than in aggressive animals. Aggressive foxes showed a sex difference in the basal blood glucose level: it was higher in males than in females. The glucose levels in aggressive females remained higher than in tame ones immediately after 10-min stress and two hours afterwards. Only aggressive females showed an excess blood glucose level over the basal one two hours after stress. The bulk group of foxes demonstrated a negative correlation between blood glucose and behavior score (response to humans) both at rest and after stress. A glucose tolerance test (GTT) was performed to compare the rates of glucose absorption from the bloodstream in silver fox females selected for behavior. Although selection for behavior did not affect glucose tolerance, judging from the area under the GTT curves, aggressive females differed from tame and unselected at the beginning of the test in longer persistence of elevated blood glucose. Thus, the selection of silver foxes for attitude to humans influences blood glucose levels at rest in both sexes and after stress in females.

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The authors declare that there are no conflicts of interest present.